Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A35 F60 I1 R1	20	36.7	1244762	99.1%	1233559	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,156,375	A→G	T341A (ACC→GCC)	yqhD →	NADPH‑dependent aldehyde reductase YqhD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,156,375	0	A	G	94.9%	131.8 / ‑1.9	39	T341A (ACC→GCC)	yqhD	NADPH‑dependent aldehyde reductase YqhD
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (16/21); total (17/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

AAACGCGATACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATAT  >  NC_000913/3156240‑3156501

aaaCGCGATACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGccaa                                                                                                                            <  2:534714/140‑3 (MQ=255)

 aaCGCGATACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCAc                                                                                                                           >  1:354893/1‑140 (MQ=255)

          ccAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGG‑TCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACt                                                                                                                 <  2:591209/140‑1 (MQ=255)

           cAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACt                                                                                                                 <  2:354893/140‑1 (MQ=255)

           cAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACt                                                                                                                 <  1:62901/140‑1 (MQ=255)

                        ctgctgCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACgg                                                                                                    <  1:260927/140‑1 (MQ=255)

                            tgcAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGc                                                                                                <  2:62953/140‑1 (MQ=255)

                                      tGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCgg                                                                                      >  2:346410/1‑140 (MQ=255)

                                         aCGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCtt                                                                                   >  2:490371/1‑140 (MQ=255)

                                             gTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCt                                                                               >  1:333086/1‑140 (MQ=255)

                                                     cATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAAc                                                                       >  2:393861/1‑140 (MQ=255)

                                                        cACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTgg                                                                    >  2:34838/1‑140 (MQ=255)

                                                          cTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGTCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGaa                                                                  <  1:598224/140‑1 (MQ=255)

                                                          cTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGaa                                                                  <  1:259011/140‑1 (MQ=255)

                                                             aaGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAagag                                                               >  1:274154/1‑140 (MQ=255)

                                                             aaGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAagag                                                               >  1:105489/1‑140 (MQ=255)

                                                                  tCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCa                                                             >  1:303344/1‑137 (MQ=255)

                                                                  tCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCa                                                             <  2:303344/137‑1 (MQ=255)

                                                                  tCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACgg                                                          <  1:553234/140‑1 (MQ=255)

                                                                  tCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACgg                                                          <  2:102871/140‑1 (MQ=255)

                                                                  tCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACgg                                                          <  1:490371/140‑1 (MQ=255)

                                                                  tCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACgg                                                          >  2:553234/1‑140 (MQ=255)

                                                                    cGATGATGAGCGTATTGACACGGCGATTGCCGCAACCCGCAATTACTTAAAGGACTTAGCCGTGCCGTGCCACTTCTCCGGCTACGGCCTGGGCGCCAGCGCCATCCCGGCTTTGCTGAAAAATCTGGAAGAGCACAGCa                                                        <  2:60816/140‑1 (MQ=255)

                                                                              cGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACt                                              >  1:444204/1‑140 (MQ=255)

                                                                                       gccgcGATTGCCACAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGaaaa                                     >  2:254480/1‑140 (MQ=255)

                                                                                        ccgcGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAAt                                    >  2:573159/1‑140 (MQ=255)

                                                                                           cgATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCAt                                 <  1:346410/140‑1 (MQ=255)

                                                                                           cgATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCAt                                 >  2:15943/1‑140 (MQ=255)

                                                                                             aTTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGGCCCTCCTCTCCGACTACGGTCTGTACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGa                               <  2:450015/140‑1 (MQ=255)

                                                                                                               ttCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAg             <  1:15943/140‑1 (MQ=255)

                                                                                                                  tttGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAgccg          <  2:105489/140‑1 (MQ=255)

                                                                                                                  tttGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAgccg          <  2:444204/140‑1 (MQ=255)

                                                                                                                  tttGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAgccg          <  1:254480/140‑1 (MQ=255)

                                                                                                                   ttGAGCAATTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAgccgc         <  2:274154/140‑1 (MQ=255)

                                                                                                                          aTTAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGtatat  <  2:333086/140‑1 (MQ=255)

                                                                                                                           ttAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAgcc           >  2:108147/1‑130 (MQ=255)

                                                                                                                           ttAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAgcc           >  2:223838/1‑130 (MQ=255)

                                                                                                                           ttAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAgcc           <  1:223838/130‑1 (MQ=255)

                                                                                                                           ttAGGCGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAgcc           <  1:108147/130‑1 (MQ=255)

                                                                                                                                cGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGt      >  2:246280/1‑130 (MQ=255)

                                                                                                                                cGTGCCGGCCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGt      <  1:246280/130‑1 (MQ=255)

AAACGCGATACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTCCGACTACGGTCTGGACGGCAGCTCCATCCCGGCTTTGCTGAAAAAACTGGAAGAGCACGGCATGACCCAACTGGGCGAAAATCATGACATTACGTTGGATGTCAGCCGCCGTATAT  >  NC_000913/3156240‑3156501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A