Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A39 F60 I1 R1	19	46.8	1599832	99.2%	1587033	138.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,221,164	G→A	A28A (GCC→GCT)	ygjH ←	putative tRNA‑binding protein YgjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,221,164	0	G	A	100.0%	119.4 / NA	35	A28A (GCC→GCT)	ygjH	putative tRNA‑binding protein YgjH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/15); total (20/15)

TTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCA  >  NC_000913/3221029‑3221300

tttCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGt                                                                                                                                      >  1:148732/1‑140 (MQ=255)

             aGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGttt                                                                                                                         <  1:127345/140‑1 (MQ=255)

                   cATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTc                                                                                                                   >  1:773579/1‑140 (MQ=255)

                           caccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCt                                                                                                           >  2:18151/1‑140 (MQ=255)

                           caccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCt                                                                                                           >  2:54416/1‑140 (MQ=255)

                             ccaccGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCttt                                                                                                         >  2:509441/1‑140 (MQ=255)

                             ccaccGTTTTCCCCATCAGCTC‑TCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTc                                                                                                        >  2:357065/1‑140 (MQ=255)

                                   ttttCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAcg                                                                                                   >  2:633692/1‑140 (MQ=255)

                                     ttCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGAcgcg                                                                                                 <  2:148732/140‑1 (MQ=255)

                                        cccATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCAt                                                                                              >  2:227887/1‑140 (MQ=255)

                                           aTCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTc                                                                                           >  2:643359/1‑140 (MQ=255)

                                           aTCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTc                                                                                           >  1:788505/1‑140 (MQ=255)

                                               gCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGa                                                                                       <  1:54416/140‑1 (MQ=255)

                                                    tcttcGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGc                                                                                  <  1:509441/140‑1 (MQ=255)

                                                         gCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAAt                                                                             <  1:643359/140‑1 (MQ=255)

                                                             tagtaCGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGc                                                                         <  2:136423/140‑1 (MQ=255)

                                                                   ggCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGc                                                                   <  2:773579/140‑1 (MQ=255)

                                                                    gCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGcc                                                                  <  1:567417/140‑1 (MQ=255)

                                                                          ggCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGtt                                                            >  1:693473/1‑140 (MQ=255)

                                                                          ggCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGtt                                                            >  1:759276/1‑140 (MQ=255)

                                                                               gTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCAt                                                       >  1:478411/1‑140 (MQ=255)

                                                                                 cACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTg                                                     <  2:478411/140‑1 (MQ=255)

                                                                                   cGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCt                                                   <  1:18151/140‑1 (MQ=255)

                                                                                    ggTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCtt                                                  <  2:693473/140‑1 (MQ=255)

                                                                                    ggTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCtt                                                  <  2:759276/140‑1 (MQ=255)

                                                                                     gTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTtc                                                 <  1:357065/140‑1 (MQ=255)

                                                                                         gCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTg                                                     >  2:775330/1‑132 (MQ=255)

                                                                                         gCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTg                                                     <  1:775330/132‑1 (MQ=255)

                                                                                                                 ccTGTACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACt                     >  1:700574/1‑140 (MQ=255)

                                                                                                                     tACGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAg                 >  1:165822/1‑140 (MQ=255)

                                                                                                                      aCGATGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGc                >  1:73010/1‑140 (MQ=255)

                                                                                                                          tGTACAGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCaaaa            >  1:303933/1‑140 (MQ=255)

                                                                                                                               aGCTTGTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGtttt       <  1:792179/140‑1 (MQ=255)

                                                                                                                                    gTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCa  >  2:573726/1‑140 (MQ=255)

                                                                                                                                    gTCAGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCa  >  1:58406/1‑140 (MQ=255)

TTTCGCTTTTTGCAGATTGCATAAGACCACCACCGTTTTCCCCATCAGCTCTTCTTCGCTGTAGTACGGCACCAGGCTGGTCACGGTTTGCAGCGTTTTTTGCCCCACATCAACCTGTACGATGTACAGCTTGTCGGCGTTTTCATGGCGTTTCACTTCCACAATCTTTCCGACGCGCATTTCCAGACGTGCAAAATCAGCGTAAGCCACGGTTTCCATTGCTTCCTCCCTTAGGGTAAAATTTTACTAAACTATAGCAAAAGTTTTTCTCA  >  NC_000913/3221029‑3221300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A