Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F60 I1 R1 21 68.5 2313054 98.9% 2287610 137.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,446,743 G→A L48L (CTG→TTG)  rpsN ← 30S ribosomal subunit protein S14

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,446,7430GA100.0% 145.6 / NA 42L48L (CTG→TTG) rpsN30S ribosomal subunit protein S14
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/21);  total (21/21)

ATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGC  >  NC_000913/3446607‑3446876
                                                                                                                                        |                                                                                                                                     
aTTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACtt                                                                                                                                    <  2:765045/140‑1 (MQ=255)
   tCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTgag                                                                                                                                 >  1:984697/1‑140 (MQ=255)
         cgcATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGc                                                                                                                           >  2:986525/1‑140 (MQ=255)
               gCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCa                                                                                                                     <  1:986525/140‑1 (MQ=255)
                cGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCaa                                                                                                                    <  2:1099606/140‑1 (MQ=255)
                        cGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTtc                                                                                                            >  1:225201/1‑140 (MQ=255)
                        cGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTtc                                                                                                            >  1:759401/1‑140 (MQ=255)
                          gACTTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTtcgt                                                                                                          <  2:819749/140‑1 (MQ=255)
                               tAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGaa                                                                                                     <  1:215342/140‑1 (MQ=255)
                                    cGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGtt                                                                                                <  1:860466/140‑1 (MQ=255)
                                    cGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGtt                                                                                                <  2:984697/140‑1 (MQ=255)
                                    cGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGtt                                                                                                <  2:522172/140‑1 (MQ=255)
                                     ggCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTc                                                                                               >  1:790938/1‑140 (MQ=255)
                                     ggCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTc                                                                                               >  2:309122/1‑140 (MQ=255)
                                       cTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGaa                                                                                                     >  1:678841/1‑132 (MQ=255)
                                       cTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGaa                                                                                                     <  2:678841/132‑1 (MQ=255)
                                       cTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGtt                                                                                                <  2:955440/137‑1 (MQ=255)
                                       cTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGtt                                                                                                >  1:955440/1‑137 (MQ=255)
                                       cTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTcac                                                                                             >  1:909693/1‑140 (MQ=255)
                                       cTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTcac                                                                                             >  1:579841/1‑140 (MQ=255)
                                       cTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTcac                                                                                             >  2:1103957/1‑140 (MQ=255)
                                         cAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACAt                                                                                           <  2:759401/140‑1 (MQ=255)
                                           aCCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCa                                                                                         <  2:320848/140‑1 (MQ=255)
                                            cccGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCag                                                                                        >  2:370937/1‑140 (MQ=255)
                                               gAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAgaga                                                                                     <  2:225201/140‑1 (MQ=255)
                                                  cTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGACAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAgatga                                                                                  <  2:82320/140‑1 (MQ=255)
                                                    tCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATc                                                                                <  2:33921/140‑1 (MQ=255)
                                                                   gCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAgcg                                                                 >  1:917574/1‑140 (MQ=255)
                                                                          acCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTcg                                                          >  2:71984/1‑140 (MQ=255)
                                                                            cTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTcgcg                                                        <  1:71984/140‑1 (MQ=255)
                                                                               tttGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAg                                                     <  1:309122/140‑1 (MQ=255)
                                                                                   aCGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTAtt                                                 >  1:272063/1‑140 (MQ=255)
                                                                                     ggCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTa                                               <  2:272063/140‑1 (MQ=255)
                                                                                       cAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATc                                             >  1:432856/1‑140 (MQ=255)
                                                                                         gCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAg                                           >  1:872878/1‑140 (MQ=255)
                                                                                                 gCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCt                                   <  2:909693/140‑1 (MQ=255)
                                                                                                 gCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCt                                   <  1:247236/140‑1 (MQ=255)
                                                                                                                  gAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcgcg                  <  1:127216/140‑1 (MQ=255)
                                                                                                                  gAATCACGCGGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTcgcg                  >  2:451058/1‑140 (MQ=255)
                                                                                                                        cgcgGCAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCttt            >  2:702231/1‑140 (MQ=255)
                                                                                                                             cAGAGTCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCattg       >  2:50381/1‑140 (MQ=255)
                                                                                                                                  tCTGCAACTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGc  >  2:1013965/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                     
ATTTCACCGCGCATAGCGGCTTCACGGACCTTAATACGGCTCAACCCGAACTTCCGCAGGAAACCATGCGGACGACCTGTTTGACGGCAGCGGTTACGCTGACGAGACGGGCTGGAATCACGCGGCAGAGTCTGCAGCTTGAGAACAGCGTTCCAACGATCTTCGTCGGAAGCGTTCACATCAGAGATGATCGCTTTCAGTTCAGCGCGTTTCGCGAAGTATTTATCAGCTAAAGCTACGCGTTTTACTTCGCGTGCTTTCATTGATTGC  >  NC_000913/3446607‑3446876

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A