Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A43 F60 I1 R1	18	59.0	2023072	99.0%	2002841	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,058,101	G→A	noncoding (2/76 nt)	asnW ←	tRNA‑Asn

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,058,101	0	G	A	100.0%	134.4 / NA	39	noncoding (2/76 nt)	asnW	tRNA‑Asn
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (25/14); total (25/14)

TAAAAATAATTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTT  >  NC_000913/2057965‑2058228

tAAAAATAATTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAAt                                                                                                                              >  1:412025/1‑140 (MQ=255)

 aaaaaTAATTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATc                                                                                                                             >  2:943182/1‑140 (MQ=255)

          ttttatttttTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACg                                                                                                                    <  2:412025/140‑1 (MQ=255)

            ttatttttTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAg                                                                                                                  <  2:508412/140‑1 (MQ=255)

                          ttttGAGACTCATAGAGATGTCTCAAAACTAAAATATGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGa                                                                                                    <  1:620074/140‑1 (MQ=255)

                            ttGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCg                                                                                                               >  1:788290/1‑127 (MQ=255)

                            ttGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCg                                                                                                               <  2:788290/127‑1 (MQ=255)

                              gagaCTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGcc                                                                                                <  2:696009/140‑1 (MQ=255)

                                            tGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCa                                                                                  >  2:531626/1‑140 (MQ=255)

                                             gTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGcccaccca                                                                                          >  1:377157/1‑131 (MQ=255)

                                             gTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGcccaccca                                                                                          <  2:377157/131‑1 (MQ=255)

                                             gTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCaa                                                                                 >  2:584072/1‑140 (MQ=255)

                                             gTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCaa                                                                                 >  2:573779/1‑140 (MQ=255)

                                             gTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCaa                                                                                 >  2:12913/1‑140 (MQ=255)

                                             gTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCaa                                                                                 >  1:796555/1‑140 (MQ=255)

                                             gTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCaa                                                                                 >  1:838116/1‑140 (MQ=255)

                                              tctcAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGcc                                                                                                <  2:161418/124‑1 (MQ=255)

                                              tctcAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGcc                                                                                                >  1:161418/1‑124 (MQ=255)

                                               ctcAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAg                                                                               >  2:719674/1‑140 (MQ=255)

                                                tcAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGc                                                                              >  1:137439/1‑140 (MQ=255)

                                                           aTTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTaa                                                                   <  2:137439/140‑1 (MQ=255)

                                                                     tctGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATc                                                         <  1:12913/140‑1 (MQ=255)

                                                                          cTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTg                                                    <  2:838116/140‑1 (MQ=255)

                                                                          cTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTg                                                    <  1:584072/140‑1 (MQ=255)

                                                                           tGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGc                                                   >  1:956078/1‑140 (MQ=255)

                                                                           tGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGc                                                   >  1:695319/1‑140 (MQ=255)

                                                                             gACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTg                                                 >  2:129722/1‑140 (MQ=255)

                                                                              aCTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGa                                                >  2:887627/1‑140 (MQ=255)

                                                                              aCTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGa                                                >  2:606918/1‑140 (MQ=255)

                                                                                                cGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTc                              >  1:596643/1‑140 (MQ=255)

                                                                                                   aTTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGtt                           <  1:977141/140‑1 (MQ=255)

                                                                                                     tAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTa                         >  1:306822/1‑140 (MQ=255)

                                                                                                     tAACAGTCCGCCGTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTa                         <  2:796555/140‑1 (MQ=255)

                                                                                                            ccgccgTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGac                  >  1:639903/1‑140 (MQ=255)

                                                                                                            ccgccgTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGac                  >  2:622001/1‑140 (MQ=255)

                                                                                                             cgccgTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGaca                 >  2:972850/1‑140 (MQ=255)

                                                                                                             cgccgTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTGGAAATCGTTTGCAGAAATAATCTGCATTTTGCCGTTTATTCCGaca                 >  2:457329/1‑140 (MQ=255)

                                                                                                               ccgTTCTACCGACTGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGAcaca               <  1:719674/140‑1 (MQ=255)

                                                                                                                            tGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCttttttt  >  1:25551/1‑140 (MQ=255)

                                                                                                                            tGAACTACAGAGAAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCttttttt  >  2:193424/1‑140 (MQ=255)

TAAAAATAATTTTTATTTTTTAACTGTTTTGAGACTCATAGAGATGTCTCAAAACTAAAATTTGGCTCCTCTGACTGGACTCGAACCAGTGACATACGGATTAACAGTCCGCCGTTCTACCGACTGAACTACAGAGGAATCGTGAGAACGAGGCGAATATTAGCGATGCCCACCCACAATGTCAAAGCCTGTTTTTTAAATTTGAAATCGTTTGCTGAAATAATCTGCATTTTGTCGTTTATTCCGACACAACTGGCTTTTTTT  >  NC_000913/2057965‑2058228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A