Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A43 F60 I1 R1 18 59.0 2023072 99.0% 2002841 138.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,173,3610C.100.0% 162.1 / NA 38pseudogene (918/1358 nt)gatCgalactitol‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (24/14);  total (24/14)
*NC_0009132,173,3620C.100.0% 162.1 / NA 38pseudogene (917/1358 nt)gatCgalactitol‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (24/14);  total (24/14)

TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCC  >  NC_000913/2173222‑2173494
                                                                                                                                            ||                                                                                                                                    
tttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATaccac                                                                                                                                       <  2:257903/140‑1 (MQ=255)
                   ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCagcagc                                                                                                                  <  2:121591/140‑1 (MQ=255)
                                  agaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAgg                                                                                                   >  1:305868/1‑140 (MQ=255)
                                     agCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa                                                                                                <  2:417022/140‑1 (MQ=255)
                                     agCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCa                                                                                                >  1:459838/1‑140 (MQ=255)
                                      gCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCaa                                                                                               <  1:837488/140‑1 (MQ=255)
                                        cGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATc                                                                                             <  2:459838/140‑1 (MQ=255)
                                              tggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAAc                                                                                       >  1:735544/1‑140 (MQ=255)
                                                gCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTc                                                                                     >  1:512042/1‑140 (MQ=255)
                                                       cGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGCGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccg                                                                              >  2:416516/1‑140 (MQ=255)
                                                       cGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccg                                                                              >  2:511815/1‑140 (MQ=255)
                                                               ggCAGCACCTGATTCCCCGGCACACAAACACAAATTAAAATGGAGAGTGGGATAAAAATC‑AGGCTTGCGGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt                                                                      <  1:502139/140‑1 (MQ=255)
                                                                          aTTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAc                                                           <  1:1006986/140‑1 (MQ=255)
                                                                                 ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACg                                                    >  2:999894/1‑140 (MQ=255)
                                                                                 ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACg                                                    >  1:979113/1‑140 (MQ=255)
                                                                                 ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACg                                                    >  1:231970/1‑140 (MQ=255)
                                                                                   cacacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAg                                                  >  2:761628/1‑140 (MQ=255)
                                                                                    acacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                 <  1:901137/140‑1 (MQ=255)
                                                                                    acaaaAACAGCAATAAAAATGGTGAGTGGGAAAAAAACC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                 <  2:979113/140‑1 (MQ=255)
                                                                                     cacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGcc                                                >  2:681580/1‑140 (MQ=255)
                                                                                     cacaAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGcc                                                >  2:304670/1‑140 (MQ=255)
                                                                                                tttAAAAGGGTGAGGGGGATAAATAGA‑AGGCTGGCCCATTCCA‑‑CCGCCGTATGTCCCGGAAGCCACTCCGGATCAAGGCGAATCCGCGGCTCGTGGCCGCCGCACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                     <  2:447917/139‑1 (MQ=255)
                                                                                                aTTAAAATGGT‑AGGGGGAAAAAAAACAAGCCTTCCCAATTCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGAAACTCCTGTCCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                     <  1:681963/140‑1 (MQ=255)
                                                                                                        ggTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTa                             >  1:247820/1‑140 (MQ=255)
                                                                                                           gAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                          >  2:306677/1‑140 (MQ=255)
                                                                                                           gAGTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                          >  1:324557/1‑140 (MQ=255)
                                                                                                           gAGTGGAAAAAAATTC‑AGGTTTGCCAATAC‑‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                          <  2:512042/140‑1 (MQ=255)
                                                                                                             gTGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcca                        >  1:34714/1‑140 (MQ=255)
                                                                                                              tGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                       >  2:487609/1‑140 (MQ=255)
                                                                                                              tGGGATAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                       >  2:519108/1‑140 (MQ=255)
                                                                                                                  aTAAAAATC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatccat                   >  1:847083/1‑140 (MQ=255)
                                                                                                                   aaaaaaCC‑AGGTTGGCGGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatg                  <  2:735544/139‑1 (MQ=255)
                                                                                                                    aaaaaTC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga                 <  2:847083/140‑1 (MQ=255)
                                                                                                                       aaTC‑AGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATgg              <  2:247820/140‑1 (MQ=255)
                                                                                                                            aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGttt          >  2:990937/1‑140 (MQ=255)
                                                                                                                              gCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa        >  2:310404/1‑140 (MQ=255)
                                                                                                                              gCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTaa        >  2:19337/1‑140 (MQ=255)
                                                                                                                               cTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAAt       <  1:416516/140‑1 (MQ=255)
                                                                                                                                   ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAcc   >  1:324862/1‑140 (MQ=255)
                                                                                                                                   ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAcc   >  1:976712/1‑140 (MQ=255)
                                                                                                                                    cGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc  <  1:100764/140‑1 (MQ=255)
                                                                                                                                    cGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc  <  1:511815/140‑1 (MQ=255)
                                                                                                                                      cgatacccccGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc  <  1:361807/134‑1 (MQ=255)
                                                                                                                                            ||                                                                                                                                    
TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAATC‑AGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCC  >  NC_000913/2173222‑2173494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A