Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A36 F60 I1 R1	17	116.8	3946860	99.2%	3915285	138.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,173,363	Δ2 bp	pseudogene (915‑916/1358 nt)	gatC ←	galactitol‑specific PTS enzyme IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,173,361	0	C	.	100.0%	355.0 / NA	83	pseudogene (918/1358 nt)	gatC	galactitol‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand): ref base C (0/0); new base . (47/36); total (47/36)
*	NC_000913	2,173,362	0	C	.	98.8%	348.9 / ‑1.8	84	pseudogene (917/1358 nt)	gatC	galactitol‑specific PTS enzyme IIC component
Reads supporting (aligned to +/- strand): ref base C (0/0); major base . (47/36); minor base A (0/1); total (47/37)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCC  >  NC_000913/2173222‑2173494

||

tttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATaccac                                                                                                                                       <  1:1237666/140‑1 (MQ=255)

tttCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATaccac                                                                                                                                       <  2:784744/140‑1 (MQ=255)

  tCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGTCGATaccaccg                                                                                                                                     <  1:293936/140‑2 (MQ=255)

       gATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATaccaccgccgta                                                                                                                                <  1:478156/140‑7 (MQ=255)

         tGCACGGCGACCGCCATCGCCACGAAGAAGCCTATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATg                                                                                                                            >  2:825429/1‑140 (MQ=255)

         tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATg                                                                                                                            >  2:684357/1‑140 (MQ=255)

         tGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATg                                                                                                                            >  2:325460/1‑140 (MQ=255)

          gCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGt                                                                                                                           <  2:1249685/140‑1 (MQ=255)

             gggCGACCGCAATCGCCACGAAGAAGCCGATGGTGGCAAGACCGCCAACCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTccc                                                                                                                        <  2:1901969/139‑1 (MQ=255)

                 gACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagca                                                                                                                    >  1:1142204/1‑140 (MQ=255)

                 gACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagca                                                                                                                    >  2:1475766/1‑140 (MQ=255)

                  acggCCCTCGCCCAGAAAAAGCCGGTGGTGGAAAGAGCGCCAAACGGAAGAACCTATTTCCCCGGCTCCCAAACAGCAATTAAAATGGTGAGTGGGTAAAAAAA‑CAGGTTGGCCG‑‑ACCCCACCGCCGTATGTCCcagcag                                                                                                                   <  2:1255681/137‑1 (MQ=255)

                  aCCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCcagcag                                                                                                                   <  2:1676528/140‑1 (MQ=255)

                   ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCagcagc                                                                                                                  <  1:651813/140‑1 (MQ=255)

                   ccGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCagcagc                                                                                                                  >  2:434622/1‑140 (MQ=255)

                      ccATCGCCACGAAGAAGCCTATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAAc                                                                                                               <  1:825429/140‑1 (MQ=255)

                         tCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGcc                                                                                                            >  1:1295557/1‑140 (MQ=255)

                            ccACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGa                                                                                                         >  2:768840/1‑140 (MQ=255)

                              aCGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATc                                                                                                       >  1:1454242/1‑140 (MQ=255)

                                  agaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAC‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAgg                                                                                                   <  2:849827/140‑1 (MQ=255)

                                   gaagCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGc                                                                                                  <  2:323491/140‑1 (MQ=255)

                                       ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGcc                                                                                                            <  2:212511/126‑1 (MQ=255)

                                       ccGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGcc                                                                                                            >  1:212511/1‑126 (MQ=255)

                                         gATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCa                                                                                            >  2:675439/1‑140 (MQ=255)

                                          aTGGTGGCAAGATCGCCAATCGGCACCCACTGTTTTCCGGGCACAGAAAAAGCTATTAAAGTGTTGAGTGGGACAAAATG‑CAGTTTCGCAGATAC‑‑TCCCACCGTATGTCCCAGCGGCAACGCCGGATCAAGGCCAATCAg                                                                                           <  2:1967083/140‑1 (MQ=255)

                                          aTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg                                                                                           <  1:1009505/140‑1 (MQ=255)

                                          aTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg                                                                                           >  1:1186841/1‑140 (MQ=255)

                                          aTGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAg                                                                                           >  1:1183287/1‑140 (MQ=255)

                                           tggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAgg                                                                                          <  2:625908/140‑1 (MQ=255)

                                            ggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATc                                                                                                       >  2:584954/1‑126 (MQ=255)

                                            ggtggCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATc                                                                                                       <  1:584954/126‑1 (MQ=255)

                                                gCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTc                                                                                     >  1:1585288/1‑140 (MQ=255)

                                                       cGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCTATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccg                                                                              >  1:942904/1‑140 (MQ=255)

                                                        gCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc                                                                             >  2:660556/1‑140 (MQ=255)

                                                        gCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc                                                                             >  1:617616/1‑140 (MQ=255)

                                                        gCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgc                                                                             >  2:816124/1‑140 (MQ=255)

                                                         ccAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGAccgcc                                                                            >  2:1791745/1‑140 (MQ=255)

                                                             aCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAAc                                                                        <  1:816124/140‑1 (MQ=255)

                                                             aCGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCAAATCAGGAACTCCTGACCGCCGAAc                                                                        <  1:684357/140‑1 (MQ=255)

                                                               ggCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACtt                                                                      <  2:878815/140‑1 (MQ=255)

                                                                 cagcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCg                                                                    >  2:1746461/1‑140 (MQ=255)

                                                                   gcaCCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGcc                                                                  >  1:269508/1‑140 (MQ=255)

                                                                        tGATTCCCCGCCACACAAACAGCAATAAAAATGGTGAGTGGAATAAAAAT‑CAGGCTGGCTGAT‑CC‑CGCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaa                                                             <  2:413514/140‑1 (MQ=255)

                                                                        tGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCTATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaa                                                             <  2:942904/140‑1 (MQ=255)

                                                                         gATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTaaa                                                            <  1:434622/140‑1 (MQ=255)

                                                                          aTTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAAc                                                           >  2:1607126/1‑140 (MQ=255)

                                                                              cccGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACt                                                       >  1:1176916/1‑140 (MQ=255)

                                                                                 ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTa                                                      >  2:1260777/1‑138 (MQ=255)

                                                                                 ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTa                                                      <  1:1260777/138‑1 (MQ=255)

                                                                                 ggCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACg                                                    >  1:1445494/1‑140 (MQ=255)

                                                                                 ggCAAACAAAAAGCAATTAAAATGTTGAGTGGGATAAAAAT‑CAGGCTGGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAGCTCCTGACCGCCGAACTTCGCCTGTAAACGACTACg                                                    <  1:1335469/140‑1 (MQ=255)

                                                                                   cacacaAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAg                                                  >  2:1251070/1‑140 (MQ=255)

                                                                                    acacaACCAGCAATTAAAAGGGGGAGGGGGAAAAAAAT‑CGGGCTCGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGACCTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                 <  2:1621759/140‑1 (MQ=255)

                                                                                    acacaAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                 <  1:1746461/140‑1 (MQ=255)

                                                                                    acacaAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                 <  1:1791745/140‑1 (MQ=255)

                                                                                    acacaAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGc                                                 >  1:118787/1‑140 (MQ=255)

                                                                                        aaaCAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGc                                             >  1:1101417/1‑140 (MQ=255)

                                                                                        aaaCAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGc                                             >  1:1567733/1‑140 (MQ=255)

                                                                                         aaaagaaaTTAAAATGGTGAGGGGGAAAAAAAC‑CAGGCTTGCGGATAC‑‑CCCCGCCGTATGTCCCAGCAGCAGCGCCGGATCAAGGCCAATCAGAACCTCCTCACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCt                                            <  2:1585288/134‑1 (MQ=255)

                                                                                             gCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGc                                        >  1:152015/1‑140 (MQ=255)

                                                                                             gCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGc                                        >  1:1956838/1‑140 (MQ=255)

                                                                                                aTTAAAATGGTGAGTGGGATAAAAAT‑CGGGCTTGCCGAT‑CC‑CCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                     <  2:1454242/140‑1 (MQ=255)

                                                                                                aTTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                     <  2:118787/140‑1 (MQ=255)

                                                                                                aTTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGAt                                     <  2:302514/140‑1 (MQ=255)

                                                                                                 ttAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATg                                    <  2:617616/140‑1 (MQ=255)

                                                                                                  tAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATgg                                   >  1:1119078/1‑140 (MQ=255)

                                                                                                  tAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATgg                                   <  2:1101417/140‑1 (MQ=255)

                                                                                                  aaaaaTGGTGAGTGGAAAAAAAAT‑CAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATgg                                   <  2:1567733/139‑1 (MQ=255)

                                                                                                      aTGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGt                               >  1:1421105/1‑140 (MQ=255)

                                                                                                        ggTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTa                             >  1:1038033/1‑140 (MQ=255)

                                                                                                        ggTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTa                             >  2:1121776/1‑140 (MQ=255)

                                                                                                         gTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaa                            <  1:1475766/140‑1 (MQ=255)

                                                                                                          ggAGGGGGAAAAAAAT‑CAGGAGTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa                           <  2:1295639/139‑1 (MQ=255)

                                                                                                          ggAGGGGGAAAAAAAC‑CAGGCTGGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTaaa                           <  2:1119078/139‑1 (MQ=255)

                                                                                                           gAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                          >  2:1565416/1‑140 (MQ=255)

                                                                                                           gAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                          >  1:1287777/1‑140 (MQ=255)

                                                                                                           gAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                          >  1:1640225/1‑140 (MQ=255)

                                                                                                           gAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                          >  2:1044061/1‑140 (MQ=255)

                                                                                                           gAGTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAc                          >  2:500620/1‑140 (MQ=255)

                                                                                                             gTGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAcca                        <  1:325460/140‑1 (MQ=255)

                                                                                                              tGGGATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                       >  1:1295639/1‑140 (MQ=255)

                                                                                                               gggATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatc                      <  2:1893174/140‑1 (MQ=255)

                                                                                                               gggATAAAAAT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccatc                      <  2:1287777/140‑1 (MQ=255)

                                                                                                                   aaaaaaT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatg                  <  1:1565416/139‑1 (MQ=255)

                                                                                                                    aaaaaT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga                 <  2:1142204/140‑1 (MQ=255)

                                                                                                                    aaaaaT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga                 <  2:1038033/140‑1 (MQ=255)

                                                                                                                    aaaaaC‑CAGGCTTGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga                 <  2:608005/140‑1 (MQ=255)

                                                                                                                     aaaaTCCAGGCTGGCCGATCCCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatga                 <  2:784299/140‑1 (MQ=255)

                                                                                                                     aaaaT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCatgat                <  2:1176916/140‑1 (MQ=255)

                                                                                                                        aT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                       >  1:533842/1‑130 (MQ=255)

                                                                                                                        aT‑CAGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAAccat                       <  2:533842/130‑1 (MQ=255)

                                                                                                                            aGGCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGttt          >  2:937029/1‑140 (MQ=255)

                                                                                                                             ggCTTGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTa         >  2:106332/1‑140 (MQ=255)

                                                                                                                                 tGCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCa     <  1:768840/140‑1 (MQ=255)

                                                                                                                                  gCCGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAc    >  2:272436/1‑140 (MQ=255)

                                                                                                                                   ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAcc   >  1:980778/1‑140 (MQ=255)

                                                                                                                                   ccGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAcc   <  1:937029/140‑1 (MQ=255)

                                                                                                                                    cGATACCA‑‑CCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCAccc  <  1:660556/140‑1 (MQ=255)

||

TTTCCACGATGCACGGCGACCGCCATCGCCACGAAGAAGCCGATGGTGGCAAGATCGCCAAACGGCAGCACCTGATTACCCGGCACACAAACAGCAATTAAAATGGTGAGTGGGATAAAAAT‑CAGGCTTGCCGATACCACCCCGCCGTATGTCCCAGCAGCAACGCCGGATCAAGGCCAATCAGGAACTCCTGACCGCCGAACTTCGCCTGTAAACGACTACGAGCCTGCTTAGCGATGGGCGTTAAACCATCCATGATGGGTTTAATCACCC  >  NC_000913/2173222‑2173494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A