Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F60 I1 R1	16	42.5	1464094	99.1%	1450917	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,793,618	G→C	G295A (GGC→GCC)	gabT →	4‑aminobutyrate aminotransferase GabT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,793,618	0	G	C	100.0%	127.2 / NA	34	G295A (GGC→GCC)	gabT	4‑aminobutyrate aminotransferase GabT
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (17/17); total (17/17)

ACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCG  >  NC_000913/2793482‑2793752

aCGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGgccgg                                                                                                                                     >  1:252746/1‑140 (MQ=255)

 cGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGgccggc                                                                                                                                    >  1:86751/1‑140 (MQ=255)

   cTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCAc                                                                                                                                  <  2:195236/140‑1 (MQ=255)

        tGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATg                                                                                                                             >  1:568059/1‑140 (MQ=255)

        tGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAAATCGATAGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATg                                                                                                                             <  1:228740/140‑1 (MQ=255)

        tGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAA‑‑‑‑ATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCggg                                                                                                                         >  1:468455/1‑140 (MQ=255)

              ggAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTa                                                                                                                       <  2:20081/140‑1 (MQ=255)

                   aGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCg                                                                                                                  >  2:447688/1‑140 (MQ=255)

                                gcCGGATCTTACCACCTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTgg                                                                                                     <  1:131401/140‑1 (MQ=255)

                                          accaccTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGa                                                                                           <  2:86751/140‑1 (MQ=255)

                                          accaccTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGa                                                                                           <  2:252746/140‑1 (MQ=255)

                                          accaccTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGa                                                                                           <  2:241143/140‑1 (MQ=255)

                                          accaccTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGa                                                                                           <  2:138454/140‑1 (MQ=255)

                                          accaccTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGa                                                                                           <  1:67350/140‑1 (MQ=255)

                                               cTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAgtg                                                                                       >  1:148175/1‑139 (MQ=255)

                                               cTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAgtg                                                                                       <  2:148175/139‑1 (MQ=255)

                                                 ttGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTg                                                                                    >  2:332539/1‑140 (MQ=255)

                                                  tGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGa                                                                                   <  1:447688/140‑1 (MQ=255)

                                                            atcgCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGa                                                                         <  2:568059/140‑1 (MQ=255)

                                                                                 gcgggcgTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGGAGTGTTGAAGGGGTTTGGGCAGGAAAATCTGCTGCaaaa                                                    >  2:450394/1‑140 (MQ=255)

                                                                                                     ggAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGa                                <  1:332539/140‑1 (MQ=255)

                                                                                                         gTAATGGATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGtt                            >  1:439308/1‑140 (MQ=255)

                                                                                                               gATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGa                      <  1:609568/140‑1 (MQ=255)

                                                                                                               gATGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGa                      >  2:192734/1‑140 (MQ=255)

                                                                                                                aTGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGAc                     >  1:243237/1‑140 (MQ=255)

                                                                                                                 tGCCGTCGCTCCAGGCGGTCTGGCCGGCTCCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACg                    >  1:126963/1‑140 (MQ=255)

                                                                                                                 tGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACg                    >  2:409512/1‑140 (MQ=255)

                                                                                                                 tGCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGAGGAAGTGGaaaaagg                    >  1:571654/1‑136 (MQ=255)

                                                                                                                  gCCGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACgg                   <  2:468455/140‑1 (MQ=255)

                                                                                                                   ccGTCGCTCCAGGCGGTCTGGCCGGCTCCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGa                  <  2:126963/140‑1 (MQ=255)

                                                                                                                    cGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGAt                 >  1:17376/1‑140 (MQ=255)

                                                                                                                    cGTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGAt                 >  1:660173/1‑140 (MQ=255)

                                                                                                                     gTCGCTCCAGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGAtt                >  1:14840/1‑140 (MQ=255)

                                                                                                                             aGGCGGTCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGa        <  1:254033/140‑1 (MQ=255)

                                                                                                                                   tCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCg  >  2:223483/1‑140 (MQ=255)

                                                                                                                                   tCTGGCCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCg  >  1:558157/1‑140 (MQ=255)

ACGCTGTTTGCGATGGAGCAGATGGGCGTTGCGCCGGATCTTACCACCTTTGCGAAATCGATCGCGGGCGGCTTCCCGCTGGCGGGCGTCACCGGGCGCGCGGAAGTAATGGATGCCGTCGCTCCAGGCGGTCTGGGCGGCACCTATGCGGGTAACCCGATTGCCTGCGTGGCTGCGCTGGAAGTGTTGAAGGTGTTTGAGCAGGAAAATCTGCTGCAAAAAGCCAACGATCTGGGGCAGAAGTTGAAAGACGGATTGCTGGCGATAGCCG  >  NC_000913/2793482‑2793752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A