Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F60 I1 R1	16	42.5	1464094	99.1%	1450917	138.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,036,733	G→A	D458D (GAC→GAT)	recJ ←	ssDNA‑specific exonuclease RecJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,036,733	0	G	A	100.0%	114.0 / NA	36	D458D (GAC→GAT)	recJ	ssDNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/19); total (17/19)

ACCGTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAAT  >  NC_000913/3036601‑3036865

aCCGTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGcc                                                                                                                               <  1:448208/140‑1 (MQ=255)

   gTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCGCTTCGCCTTGCAATAGCGAAGGATCCAGcc                                                                                                                               <  2:649540/137‑1 (MQ=255)

   gTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGcc                                                                                                                               >  1:649540/1‑137 (MQ=255)

   gTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACt                                                                                                                            <  2:580950/140‑1 (MQ=255)

   gTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGAGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACt                                                                                                                            >  2:500571/1‑140 (MQ=255)

               cTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACt                                                                                                                            >  1:446841/1‑128 (MQ=255)

               cTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACt                                                                                                                            <  2:446841/128‑1 (MQ=255)

                      aaCATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCaaa                                                                                                         <  1:339901/140‑1 (MQ=255)

                            tGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCgttg                                                                                                   >  2:648896/1‑140 (MQ=255)

                                       gCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGtt                                                                                        <  1:731360/140‑1 (MQ=255)

                                       gCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGtt                                                                                        <  1:648896/140‑1 (MQ=255)

                                            cATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAAt                                                                                   >  2:422828/1‑140 (MQ=255)

                                                 cgcAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTAt                                                                               >  1:172801/1‑139 (MQ=255)

                                                 cgcAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTAt                                                                               <  2:172801/139‑1 (MQ=255)

                                                 cgcAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATc                                                                              >  1:319405/1‑140 (MQ=255)

                                                                              ttCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCg                                                 >  2:453211/1‑140 (MQ=255)

                                                                              ttCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCg                                                 >  2:112494/1‑140 (MQ=255)

                                                                                         ttAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCa                                      <  2:319405/140‑1 (MQ=255)

                                                                                           aaCGGACCGTCTGATCCCACTTCGCCTTGCAATGGCGAAGAATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTGGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAACCCCGCCGCCATCGCATGACCGCCaaa                                    <  2:40763/140‑1 (MQ=255)

                                                                                              ggACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACtt                                 <  1:500571/140‑1 (MQ=255)

                                                                                                 ccGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGAc                                           <  1:635795/127‑1 (MQ=255)

                                                                                                 ccGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGAc                                           >  2:635795/1‑127 (MQ=255)

                                                                                                 ccGTCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAg                              >  1:62803/1‑140 (MQ=255)

                                                                                                    tCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGcat                           <  2:62803/140‑1 (MQ=255)

                                                                                                    tCTGATACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGcat                           <  1:422828/140‑1 (MQ=255)

                                                                                                        aTACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATg                       <  1:453211/140‑1 (MQ=255)

                                                                                                         ttccACTTCGCTTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCAAAACCGTTGTTGAAAGAGTTTGATTTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGc                      <  2:98112/138‑1 (MQ=255)

                                                                                                         tACCACTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGc                      <  1:112494/140‑1 (MQ=255)

                                                                                                          agcccTTCGCTTTGAAATGGCGAAGGATCCAGCCACTCAGTACCCAGTTCGCAAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGcc                     <  2:716608/136‑1 (MQ=255)

                                                                                                              cTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGcat                           <  2:100023/130‑1 (MQ=255)

                                                                                                              cTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGcat                           >  1:100023/1‑130 (MQ=255)

                                                                                                              cTTCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAggg                 >  2:479713/1‑140 (MQ=255)

                                                                                                               ttCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGcat                           <  2:5225/129‑1 (MQ=255)

                                                                                                               ttCGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGcat                           >  1:5225/1‑129 (MQ=255)

                                                                                                                ccGCCTGGGAATAGCGAAGGATCCAGCTCCTCAGTACCCAGTTCGCCAAACCGTTGTTGAAGGAGTTTAAATTTATCCTTTGCCAGCGACAATCCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTa               <  2:65739/139‑1 (MQ=255)

                                                                                                                 cGCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAAAGTTGGAATTTATCCTCTTCCAGTGACAAACCTGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGCag              >  2:480045/1‑140 (MQ=255)

                                                                                                                  gCCTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTaga             >  1:65739/1‑140 (MQ=255)

                                                                                                                    cTTGCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAgt           <  1:382557/140‑1 (MQ=255)

                                                                                                                       gCAATAGCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAgtgct        >  1:716608/1‑138 (MQ=255)

                                                                                                                             gCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAAt  <  1:460212/140‑1 (MQ=255)

                                                                                                                             gCGAAGGATCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAAt  <  1:453574/140‑1 (MQ=255)

ACCGTCAAACAGCGGCTCCGGGAACATCTGCCCCCACGGGCCAGCATCGCGCAGCAGCTGCGCCACTTCCATGGTCATTTCGGCCGGGCTTAACGGACCGTCTGATACCACTTCGCCTTGCAATAGCGAAGGGTCCAGCCACTCAGTAACCAGTTCGCCAAACCGTTGTTGAAAGAGTTTGAATTTATCCTCTTCCAGCGACAAACCCGCCGCCATCGCATGACCGCCAAACTTCAGCATCATGCCAGGGTAGAGTGTGTCTAAT  >  NC_000913/3036601‑3036865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A