Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A40 F60 I1 R1	21	90.3	3067138	99.1%	3039533	138.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,655,086	A→G	L425L (TTA→CTA)	pepB ←	aminopeptidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,655,086	0	A	G	100.0%	205.7 / NA	53	L425L (TTA→CTA)	pepB	aminopeptidase B
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (25/28); total (25/28)

TCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGG  >  NC_000913/2654950‑2655219

tcaACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAg                                                                                                                                    <  1:807349/140‑1 (MQ=255)

  aaCGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGAt                                                                                                                                  <  2:996558/140‑1 (MQ=255)

     gTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAg                                                                                                                               >  2:1191730/1‑140 (MQ=255)

       tCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCt                                                                                                                             >  1:1440456/1‑140 (MQ=255)

               cAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTgcg                                                                                                                     <  2:826225/140‑1 (MQ=255)

                aaaTGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTgcgc                                                                                                                    <  2:1440456/140‑1 (MQ=255)

                     ccTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACAcc                                                                                                               <  2:713468/140‑1 (MQ=255)

                     ccTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACAcc                                                                                                               <  2:1183449/140‑1 (MQ=255)

                     ccTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACAcc                                                                                                               <  1:1048012/140‑1 (MQ=255)

                                           gCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGAc                                                                                         >  2:1333600/1‑140 (MQ=255)

                                           gCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGAc                                                                                         >  1:774672/1‑140 (MQ=255)

                                           gCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGAc                                                                                         >  1:62694/1‑140 (MQ=255)

                                              tACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCAc                                                                                      >  1:1307754/1‑140 (MQ=255)

                                                cAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTg                                                                                    >  1:473483/1‑140 (MQ=255)

                                                cAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTg                                                                                    >  1:906011/1‑140 (MQ=255)

                                                 aaGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGt                                                                                   <  1:360337/140‑1 (MQ=255)

                                                     gATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAgcgc                                                                                                 >  1:61527/1‑122 (MQ=255)

                                                     gATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAgcgc                                                                                                 <  2:61527/122‑1 (MQ=255)

                                                     gATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCaa                                                                               >  1:488120/1‑140 (MQ=255)

                                                     gATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCaa                                                                               >  2:1039045/1‑140 (MQ=255)

                                                     gATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCaa                                                                               >  1:1328289/1‑140 (MQ=255)

                                                        tCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCg                                                                            >  1:595207/1‑140 (MQ=255)

                                                         cGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCgg                                                                           <  2:488120/140‑1 (MQ=255)

                                                           cAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCg                                                                            <  1:1307891/137‑1 (MQ=255)

                                                           cAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCg                                                                            >  2:1307891/1‑137 (MQ=255)

                                                             aTTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCt                                                                       <  2:774672/140‑1 (MQ=255)

                                                             aTTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCt                                                                       <  2:1307754/140‑1 (MQ=255)

                                                                     aaTTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTa                                                               <  2:473483/140‑1 (MQ=255)

                                                                     aaTTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTa                                                               <  2:597988/140‑1 (MQ=255)

                                                                     aaTTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTa                                                               <  2:617292/140‑1 (MQ=255)

                                                                               aTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAg                                                     >  2:101891/1‑140 (MQ=255)

                                                                                tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGc                                                    <  1:1039045/140‑1 (MQ=255)

                                                                                tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGc                                                    >  2:1306347/1‑140 (MQ=255)

                                                                                          ggATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATg                                          >  2:1309588/1‑140 (MQ=255)

                                                                                          ggATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATg                                          >  1:402127/1‑140 (MQ=255)

                                                                                              aaGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGcagc                                      <  1:1333600/140‑1 (MQ=255)

                                                                                                ggCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGcagcca                                    <  1:101891/140‑1 (MQ=255)

                                                                                                ggCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGcagcca                                    <  2:62694/140‑1 (MQ=255)

                                                                                                  cGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGcagccagc                                  >  1:797670/1‑140 (MQ=255)

                                                                                                     ttACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCtt                               <  2:402127/140‑1 (MQ=255)

                                                                                                      tACGCCGCATCCGGCATATTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTg                              >  1:263722/1‑140 (MQ=255)

                                                                                                                       atTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTcaacaa             <  2:263722/140‑1 (MQ=255)

                                                                                                                       atTAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCACAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTcaacaa             <  1:441322/140‑1 (MQ=255)

                                                                                                                         tAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGa                          >  2:350943/1‑125 (MQ=255)

                                                                                                                         tAGTTTACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGa                          <  1:350943/125‑1 (MQ=255)

                                                                                                                              tACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGa      <  2:484583/140‑1 (MQ=255)

                                                                                                                              tACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGa      >  2:670269/1‑140 (MQ=255)

                                                                                                                              tACGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGa      <  2:1001508/140‑1 (MQ=255)

                                                                                                                                cGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACa    >  1:853266/1‑140 (MQ=255)

                                                                                                                                cGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACa    >  2:1094244/1‑140 (MQ=255)

                                                                                                                                cGCCGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATAAGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACa    >  1:1118281/1‑140 (MQ=255)

                                                                                                                                  ccGTTAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAgg  <  1:1306347/140‑1 (MQ=255)

                                                                                                                                     ttAGCAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACaaa            <  1:704544/127‑1 (MQ=255)

TCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTTCTCAACAAAGTGCGACAGG  >  NC_000913/2654950‑2655219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A