Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A21 F60 I1 R1 16 125.0 4250738 99.1% 4212481 138.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,124,257 T→C E401E (GAA→GAG)  glcF ← glycolate dehydrogenase, putative iron‑sulfur subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,124,2570TC100.0% 296.4 / NA 75E401E (GAA→GAG) glcFglycolate dehydrogenase, putative iron‑sulfur subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (38/37);  total (38/37)

TCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGC  >  NC_000913/3124122‑3124393
                                                                                                                                       |                                                                                                                                        
tCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTa                                                                                                                                      >  2:687249/1‑140 (MQ=255)
tCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTa                                                                                                                                      <  1:1726436/140‑1 (MQ=255)
 cATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTAc                                                                                                                                     <  2:298317/140‑1 (MQ=255)
        aaCAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCa                                                                                                                              >  1:1032455/1‑140 (MQ=255)
         aCAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCaa                                                                                                                             <  1:311179/140‑1 (MQ=255)
          cAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAAt                                                                                                                            >  1:1034119/1‑140 (MQ=255)
           aGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATc                                                                                                                           <  2:1032455/140‑1 (MQ=255)
           aGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATc                                                                                                                           <  1:1789281/140‑1 (MQ=255)
               aTGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCGCTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTGCAATTTCAATCGAGt                                                                                                                       <  2:839159/140‑1 (MQ=255)
               aTGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGt                                                                                                                       <  2:1830847/140‑1 (MQ=255)
                tGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTg                                                                                                                      >  2:757971/1‑140 (MQ=255)
                     aCAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGca                                                                                                                 >  2:968202/1‑140 (MQ=255)
                     aCAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGca                                                                                                                 <  1:809634/140‑1 (MQ=255)
                       aGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGATAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGcaca                                                                                                               <  1:1805649/140‑1 (MQ=255)
                         aCCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACg                                                                                                                   <  2:1736627/134‑1 (MQ=255)
                         aCCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACg                                                                                                                   >  1:1736627/1‑134 (MQ=255)
                              ttATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTAc                                                                                                        >  2:1199691/1‑140 (MQ=255)
                              ttATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTAc                                                                                                        >  1:901849/1‑140 (MQ=255)
                                   tttCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAgg                                                                                                           <  2:499929/132‑1 (MQ=255)
                                   tttCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAgg                                                                                                           >  1:499929/1‑132 (MQ=255)
                                    ttCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCgg                                                                                                  <  1:757971/140‑1 (MQ=255)
                                          gcCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCg                                                                                            <  1:1279957/140‑1 (MQ=255)
                                               ttCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGa                                                                                       <  2:901849/140‑1 (MQ=255)
                                                    gACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGt                                                                                  <  2:1034119/140‑1 (MQ=255)
                                                         gCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGc                                                                             >  2:429262/1‑140 (MQ=255)
                                                         gCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGc                                                                             >  2:603731/1‑140 (MQ=255)
                                                         gCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGc                                                                             >  1:1233380/1‑140 (MQ=255)
                                                         gCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGc                                                                             >  1:1471131/1‑140 (MQ=255)
                                                          cGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGt                                                                                  >  1:1693586/1‑134 (MQ=255)
                                                          cGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGt                                                                                  <  2:1693586/134‑1 (MQ=255)
                                                          cGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCa                                                                            <  2:1233380/140‑1 (MQ=255)
                                                          cGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCa                                                                            >  1:2043423/1‑140 (MQ=255)
                                                          cGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCa                                                                            >  2:447216/1‑140 (MQ=255)
                                                          cGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCa                                                                            >  2:1781578/1‑140 (MQ=255)
                                                                 aatTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATg                                                                     >  1:82623/1‑140 (MQ=255)
                                                                       aCTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCg                                                               <  1:609166/140‑1 (MQ=255)
                                                                           gCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGa                                                           >  2:1224156/1‑140 (MQ=255)
                                                                            ccATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGAc                                                          >  1:1699683/1‑140 (MQ=255)
                                                                               tttGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGAt                                                       <  1:1199691/140‑1 (MQ=255)
                                                                               tttGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGAt                                                       <  1:968202/140‑1 (MQ=255)
                                                                                     ggCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTc                                                 >  1:1825305/1‑140 (MQ=255)
                                                                                     ggCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTc                                                 >  1:1469236/1‑140 (MQ=255)
                                                                                     ggCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTc                                                 >  1:547420/1‑140 (MQ=255)
                                                                                       cTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCg                                               >  1:1299012/1‑140 (MQ=255)
                                                                                          aGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGtt                                            <  1:1311361/140‑1 (MQ=255)
                                                                                          aGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGtt                                            >  1:1752542/1‑140 (MQ=255)
                                                                                          aGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGtt                                            <  1:1224156/140‑1 (MQ=255)
                                                                                                aCTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGc                                      >  1:1398140/1‑140 (MQ=255)
                                                                                                aCTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGc                                      >  1:1170089/1‑140 (MQ=255)
                                                                                                aCTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGc                                      >  1:872746/1‑140 (MQ=255)
                                                                                                     aGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCACCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTcc                                 >  2:1364186/1‑140 (MQ=255)
                                                                                                       ttttCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAg                               <  1:1525096/140‑1 (MQ=255)
                                                                                                         ttCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAgcg                             >  1:2103707/1‑140 (MQ=255)
                                                                                                          tCATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAgcgc                            <  1:1993974/140‑1 (MQ=255)
                                                                                                           cATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGca                           <  2:1299012/140‑1 (MQ=255)
                                                                                                           cATTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGca                           >  2:875310/1‑140 (MQ=255)
                                                                                                            aTTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGcat                          <  1:982769/140‑1 (MQ=255)
                                                                                                            aTTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGca                           >  2:563267/1‑139 (MQ=255)
                                                                                                            aTTTTGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGca                           <  1:563267/139‑1 (MQ=255)
                                                                                                               ttGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGcattca                       <  1:875310/140‑1 (MQ=255)
                                                                                                               ttGTTATTCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCACCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGcattca                       <  1:1364186/140‑1 (MQ=255)
                                                                                                                     ttCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGt                 <  2:82623/140‑1 (MQ=255)
                                                                                                                     ttCCTTTTCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGt                 <  2:1170089/140‑1 (MQ=255)
                                                                                                                          tttCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGcattca                       >  2:165771/1‑129 (MQ=255)
                                                                                                                          tttCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGcattca                       <  1:165771/129‑1 (MQ=255)
                                                                                                                          tttCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATcc            >  2:1807012/1‑140 (MQ=255)
                                                                                                                          tttCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATcc            >  2:1553838/1‑140 (MQ=255)
                                                                                                                          tttCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATcc            >  2:1274624/1‑140 (MQ=255)
                                                                                                                          tttCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATcc            >  2:1034277/1‑140 (MQ=255)
                                                                                                                          tttCAAGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATcc            >  1:1569117/1‑140 (MQ=255)
                                                                                                                              aaGGGCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCa        <  1:1807012/140‑1 (MQ=255)
                                                                                                                                gggCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGc      <  2:1860484/140‑1 (MQ=255)
                                                                                                                                  gCTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTg    <  2:1203385/140‑1 (MQ=255)
                                                                                                                                   cTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTgg   <  2:547420/140‑1 (MQ=255)
                                                                                                                                   cTTGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTgg   <  2:1469236/140‑1 (MQ=255)
                                                                                                                                    ttGCTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGc  <  2:872746/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
TCATCGGCAACAGCAATGGAAACAGACCAGTTATTTTTCTGCGCCTCTTCCTGACCTGCGGCAATAATTGCACTCGCCATTTGCTGGCTAAGAATGACTTTAGTTTTCATTTTGTTATTCCTTTTCAAGGGCTTGTTCTACAATTTCAATCCAGTGACGCACAGAGGTACGACCGGCGCTCGCCAGATGCGTCTGGCAACCAATGTTGGCGGTGACGATCATTTCCGGTTTGCCGCTTTCCAGCGCATTCATTTTGTTATCCCGCAGCTGGC  >  NC_000913/3124122‑3124393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A