Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F28 I1 R1
|
114 |
64.8 |
2195608 |
99.1% |
2175847 |
138.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_000913 |
2,682,157 |
(C)7→8 |
coding (589/3282 nt) |
yphG ← |
DUF5107 domain‑containing protein YphG |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,682,150 | 1 | . | C | 97.6%
| 144.8
/ ‑3.2
| 41 | coding (596/3282 nt) | yphG | DUF5107 domain‑containing protein YphG |
| Reads supporting (aligned to +/- strand): ref base . (0/1); new base C (25/15); total (25/16) |
| Fisher's exact test for biased strand distribution p-value = 3.90e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01 |
CGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGT > NC_000913/2682018‑2682278
|
cGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑ccccc‑cc > 2:852942/1‑140 (MQ=255)
cGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTCCCCCCC‑CCTTTCACTGCCGGGTTgg > 1:681234/1‑140 (MQ=255)
cGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGc > 1:878376/1‑140 (MQ=255)
tagtCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCccacc > 1:790552/1‑140 (MQ=255)
gtCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCcaccac > 1:928377/1‑140 (MQ=255)
tCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACcaca > 2:464666/1‑140 (MQ=255)
tGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATg > 1:406974/1‑140 (MQ=255)
tagtaAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGCTGGCCCACCACAAGAAATGAc > 2:53394/1‑140 (MQ=255)
agtaAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAcg > 1:908559/1‑140 (MQ=255)
aGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCCGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGAccccgc > 2:761690/1‑135 (MQ=255)
gcgggTAGCGAGGGGAAAGGGGGGGGCGGCCCGTTTCCCGTGACCAAACCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtg < 2:445331/137‑1 (MQ=255)
gCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtg > 1:445331/1‑140 (MQ=255)
gCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCggcgtg > 2:170230/1‑140 (MQ=255)
tggggagggggAAGGGGGAGACGGCCCTTTTGCGGTAATAAACCACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt < 1:316159/133‑1 (MQ=255)
tAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt > 2:741813/1‑140 (MQ=255)
tAGAGAGGGGAAAAGCGGAGACGGCCCTTTCGCCGTAATAAACCACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt < 1:758243/140‑1 (MQ=255)
gggggggggggAAGGCGGAGCCGCCCCTTTCCCGGTGACCAACCCCCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGtt < 2:688831/133‑1 (MQ=255)
aGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTc > 2:866455/1‑140 (MQ=255)
gCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTcc > 2:190851/1‑140 (MQ=255)
aTGGGGAAAGCGGAGACGGCCCGTTTACCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGt > 2:266557/1‑140 (MQ=255)
gCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGa > 2:571888/1‑140 (MQ=255)
gCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGa > 2:766293/1‑140 (MQ=255)
cccccgccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTc < 2:673395/137‑1 (MQ=255)
gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc > 1:253560/1‑140 (MQ=255)
gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc > 1:521733/1‑140 (MQ=255)
gccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGGTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgc > 2:1013980/1‑140 (MQ=255)
ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg > 2:471950/1‑140 (MQ=255)
ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg > 2:1029867/1‑140 (MQ=255)
ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg < 1:950724/140‑1 (MQ=255)
ccgTTACATCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcg > 1:179093/1‑140 (MQ=255)
cgTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCCGCCTTTCACGGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGgcgc < 2:436846/140‑1 (MQ=255)
tCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGa < 2:928377/140‑1 (MQ=255)
tCCGGCGGGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGa < 2:1003435/140‑1 (MQ=255)
cggcggGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGaaa < 1:471950/140‑1 (MQ=255)
ggcggGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAAc > 2:418721/1‑140 (MQ=255)
ggcggGAAGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAAc < 2:253560/140‑1 (MQ=255)
aaGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtca > 1:520216/1‑140 (MQ=255)
aGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat < 2:179093/140‑1 (MQ=255)
aGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat < 1:495329/140‑1 (MQ=255)
aGACGCTCTGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGtcat < 1:418721/140‑1 (MQ=255)
tctGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTg < 2:878376/140‑1 (MQ=255)
tctGATGCCCTT‑‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCAGCGTGGCGTTCCCGTTATCGACGCGGCTGGCGATTTCCAGCACCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGt < 2:385082/140‑1 (MQ=255)
ctGATGCCCTTC‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGt < 1:190851/140‑1 (MQ=255)
|
CGAGAAATGTCCACTCCAGCGGAGTAGTCCACTTTGTAGTAAGTGCCGGTAGCGATGGGGAAAGCGGAGACGGCCCGTTTGCCGTGATCAAACACCGCCGTTACATCCGGCGGGAAGACGCTCTGATGCCCTT‑‑CCCCC‑CCTTTCACTGCCGGGTTGGCCCACCACAAGAAATGACGCGGCGTGGCGTTCCCGTTATAGACGCGGCTGGCGATTTCCAGCGCCGCCCGGTCAGGGCGCAGGGTGAAACCTGTCATCACCTGT > NC_000913/2682018‑2682278
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
