Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A23 F30 I1 R1	20	57.7	1952418	99.1%	1934846	138.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,353,206	C→A	Q504H (CAG→CAT)	lysU ←	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,353,206	0	C	A	100.0%	142.9 / NA	41	Q504H (CAG→CAT)	lysU	lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (16/25); total (16/25)

CTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTT  >  NC_000913/4353075‑4353342

ctTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGgcg                                                                                                                                  >  1:927051/1‑140 (MQ=255)

  tttAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCa                                                                                                                                <  2:77282/140‑1 (MQ=255)

  tttAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCa                                                                                                                                <  2:469012/140‑1 (MQ=255)

    tAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATc                                                                                                                              >  2:391251/1‑140 (MQ=255)

       gAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACTAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGcc                                                                                                                           <  2:63816/140‑1 (MQ=255)

         gCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCgg                                                                                                                         <  2:831643/140‑1 (MQ=255)

         gCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCgg                                                                                                                         <  1:893730/140‑1 (MQ=255)

          cTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCggg                                                                                                                        <  2:918264/140‑1 (MQ=255)

           tGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGa                                                                                                                       >  1:476096/1‑140 (MQ=255)

            gTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGaa                                                                                                                      >  2:742681/1‑140 (MQ=255)

                ttGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAgaga                                                                                                                  <  2:273879/140‑1 (MQ=255)

                  gAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACAATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAAt                                                                                                                <  1:597341/140‑1 (MQ=255)

                   aGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATa                                                                                                               <  1:391251/140‑1 (MQ=255)

                   aGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATa                                                                                                               <  2:45219/140‑1 (MQ=255)

                     tGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAAc                                                                                                             >  1:866006/1‑140 (MQ=255)

                        aCCCGCTGGTTATCGTGAAACGAAGTGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTc                                                                                                          <  1:457062/140‑1 (MQ=255)

                        aCCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTc                                                                                                          <  1:881450/140‑1 (MQ=255)

                        aCCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTc                                                                                                          <  2:513765/140‑1 (MQ=255)

                          ccGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTcgc                                                                                                        >  2:605881/1‑140 (MQ=255)

                           cGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTcgcg                                                                                                       <  1:390303/140‑1 (MQ=255)

                                     cGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATgg                                                                                             >  1:932049/1‑140 (MQ=255)

                                      gTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGc                                                                                            <  2:866006/140‑1 (MQ=255)

                                           aCGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGc                                                                                            >  2:434923/1‑135 (MQ=255)

                                           aCGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGACTGTTa                                                                                       >  2:89046/1‑140 (MQ=255)

                                           aCGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGCTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGc                                                                                            <  1:434923/135‑1 (MQ=255)

                                               aGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTaa                                                                                   >  2:630951/1‑140 (MQ=255)

                                                gCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTaaa                                                                                  <  2:619985/140‑1 (MQ=255)

                                                                    aTTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGa                                                              <  1:630951/140‑1 (MQ=255)

                                                                       tGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAc                                                           <  1:742681/140‑1 (MQ=255)

                                                                                      gAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAg                                            <  1:878648/140‑1 (MQ=255)

                                                                                        aGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCg                                          >  2:158244/1‑140 (MQ=255)

                                                                                                 cTGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATCCCCAGACCAGCGGTTggcggc                                 <  2:750966/140‑1 (MQ=255)

                                                                                                  tGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCa                                <  1:673035/140‑1 (MQ=255)

                                                                                                  tGACTGCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCa                                <  2:816324/140‑1 (MQ=255)

                                                                                                       gCTTCGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCa                           >  1:591947/1‑140 (MQ=255)

                                                                                                           cGTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATAtt                       >  2:537034/1‑140 (MQ=255)

                                                                                                            gTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTc                      <  2:942561/140‑1 (MQ=255)

                                                                                                            gTTCATTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTc                      <  2:476096/140‑1 (MQ=255)

                                                                                                                aTTAAAGTGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgc                  >  1:885325/1‑140 (MQ=255)

                                                                                                                       tGAAATTTATTTATGTGGGCGCATCGCCTGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCa           >  1:599227/1‑140 (MQ=255)

                                                                                                                       tGAAATTTATTTATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCa           >  1:610703/1‑140 (MQ=255)

                                                                                                                                tttATGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCtt  <  2:610703/140‑1 (MQ=255)

CTTTTAGGAGCTGTAGTTGAGTGGACCCGCTGGTTATCGTGAAACGAAGCGTCTCTCCTCAAAACTGGATTTGCGGAACGATGAACGAAGCAGTCGCCTGACTGCTTCGTTCATTAAAGTGAAATTTATTTCTGTGGGCGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAGCGCAGTCACGTAATCTT  >  NC_000913/4353075‑4353342

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A