Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F30 I1 R1	27	113.4	3878132	98.9%	3835472	139.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,360,559	A→C	V459G (GTT→GGT)	cadC ←	DNA‑binding transcriptional activator CadC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,360,559	0	A	C	99.4%	665.6 / ‑4.3	178	V459G (GTT→GGT)	cadC	DNA‑binding transcriptional activator CadC
Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (78/99); total (78/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01

Only 100 of 178 total aligned reads displayed.

GGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATAATGGACAGGTT  >  NC_000913/4360427‑4360692

  tACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCaa                                                                                                                              >  1:779837/1‑140 (MQ=255)

     aacaTAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCa                                                                                                                           <  1:15233/140‑1 (MQ=255)

     aacaTAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCa                                                                                                                           <  1:1430216/140‑1 (MQ=255)

     aacaTAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCa                                                                                                                           <  1:689900/140‑1 (MQ=255)

     aacaTAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCa                                                                                                                           >  2:1430216/1‑140 (MQ=255)

        aTAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAaca                                                                                                                        <  1:1684453/140‑1 (MQ=255)

         tAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAacac                                                                                                                       <  2:1507803/140‑1 (MQ=255)

         tAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAacac                                                                                                                       <  1:1326245/140‑1 (MQ=255)

          aaGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAAcaca                                                                                                                      >  2:801191/1‑140 (MQ=255)

           aGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACAt                                                                                                                     >  2:845276/1‑140 (MQ=255)

            ggAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATa                                                                                                                    >  2:1363346/1‑140 (MQ=255)

            ggAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATa                                                                                                                    >  2:1816710/1‑140 (MQ=255)

              aaCAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATagt                                                                                                                  <  2:301148/140‑3 (MQ=255)

              aaCAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAAt                                                                                                                  <  1:1801524/140‑1 (MQ=255)

                 aGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCTTTGCCAAGCAACACATAATTTa                                                                                                               >  1:890786/1‑140 (MQ=255)

                 aGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTa                                                                                                               >  2:70487/1‑140 (MQ=255)

                 aGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTa                                                                                                               >  1:802663/1‑140 (MQ=255)

                  gAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAg                                                                                                              >  1:14575/1‑140 (MQ=255)

                  gAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAg                                                                                                              <  2:802663/140‑1 (MQ=255)

                   aaGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAaca                                                                                                                        <  2:774872/129‑1 (MQ=255)

                        tGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGa                                                                                                        >  2:1449523/1‑140 (MQ=255)

                         ggAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGAc                                                                                                       <  2:1458540/140‑1 (MQ=255)

                         ggAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGAc                                                                                                       <  1:1449523/140‑1 (MQ=255)

                               taCCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCa                                                                                                 <  2:715523/140‑1 (MQ=255)

                               taCCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCa                                                                                                 <  1:1196185/140‑1 (MQ=255)

                                aCCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCaa                                                                                                >  1:379273/1‑140 (MQ=255)

                                    ttttCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGa                                                                                                        >  1:1898394/1‑128 (MQ=255)

                                    ttttCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGa                                                                                                        <  2:1898394/128‑1 (MQ=255)

                                    ttttCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGAc                                                                                                       <  2:1853307/129‑1 (MQ=255)

                                    ttttCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGAc                                                                                                       >  1:1853307/1‑129 (MQ=255)

                                    ttttCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATc                                                                                            >  2:1008146/1‑140 (MQ=255)

                                    ttttCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATc                                                                                            >  1:567391/1‑140 (MQ=255)

                                     tttCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCa                                                                                           >  2:1772490/1‑140 (MQ=255)

                                     tttCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCa                                                                                           <  1:1581713/140‑1 (MQ=255)

                                       tCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGa                                                                                                        >  2:552003/1‑125 (MQ=255)

                                         aaTCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAgg                                                                                                         <  1:1137873/122‑1 (MQ=255)

                                         aaTCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAgg                                                                                                         >  2:1137873/1‑122 (MQ=255)

                                         aaTCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGc                                                                                       <  2:534745/140‑1 (MQ=255)

                                         aaTCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGc                                                                                       <  2:337924/140‑1 (MQ=255)

                                         aaTCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGc                                                                                       <  1:1454207/140‑1 (MQ=255)

                                          aTCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGcc                                                                                      <  1:1066883/140‑1 (MQ=255)

                                              aGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTa                                                                                  >  1:1276044/1‑140 (MQ=255)

                                                tAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTAtt                                                                                >  1:835775/1‑140 (MQ=255)

                                                tAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCCGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTAtt                                                                                >  2:489270/1‑140 (MQ=255)

                                                     ggTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCg                                                                           >  1:70836/1‑140 (MQ=255)

                                                     ggTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCg                                                                           <  1:1014695/140‑1 (MQ=255)

                                                     ggTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCg                                                                           <  1:388041/140‑1 (MQ=255)

                                                      gtgtTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGc                                                                          <  1:56000/140‑1 (MQ=255)

                                                      gtgtTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGc                                                                          <  1:1816710/140‑1 (MQ=255)

                                                        gtTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCt                                                                        >  2:1749713/1‑140 (MQ=255)

                                                        gtTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCt                                                                        >  2:1639469/1‑140 (MQ=255)

                                                         tttGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGcc                                                                         <  1:545484/138‑1 (MQ=255)

                                                         tttGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTg                                                                       >  1:1043344/1‑140 (MQ=255)

                                                         tttGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTg                                                                       >  2:621196/1‑140 (MQ=255)

                                                         tttGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTg                                                                       >  1:188218/1‑140 (MQ=255)

                                                         tttGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTg                                                                       >  2:1690671/1‑140 (MQ=255)

                                                          ttGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTgg                                                                      <  1:1632886/140‑1 (MQ=255)

                                                          ttGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTgg                                                                      >  2:308955/1‑140 (MQ=255)

                                                             ccccTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTaa                                                                   <  2:926071/140‑1 (MQ=255)

                                                               ccTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGa                                                                 <  2:1043344/140‑1 (MQ=255)

                                                                 tGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACt                                                               >  2:1190587/1‑140 (MQ=255)

                                                                  gggCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTc                                                              <  1:583617/140‑1 (MQ=255)

                                                                  gggCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTc                                                              <  1:621196/140‑1 (MQ=255)

                                                                    gCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCAt                                                            <  1:533193/140‑1 (MQ=255)

                                                                       tAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTaa                                                                   >  2:381751/1‑130 (MQ=255)

                                                                          aTTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGttt                                                      <  1:1190587/140‑1 (MQ=255)

                                                                          aTTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGttt                                                      <  2:1336708/140‑1 (MQ=255)

                                                                            tAAAGGCTGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGtttta                                                    <  2:268330/140‑1 (MQ=255)

                                                                            tAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGtttta                                                    <  2:835775/140‑1 (MQ=255)

                                                                              aaGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGttttacc                                                  >  2:621988/1‑140 (MQ=255)

                                                                               aGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGttttacct                                                 <  2:221434/140‑1 (MQ=255)

                                                                                 gCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGttttaccttt                                               <  1:866777/140‑1 (MQ=255)

                                                                                     tGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGttttaccttttacc                                           >  2:1916239/1‑140 (MQ=255)

                                                                                       agaTATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGttttaccttttacc                                           <  2:415028/138‑1 (MQ=255)

                                                                                       agaTATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGttttaccttttacc                                           >  1:415028/1‑138 (MQ=255)

                                                                                         atatGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCagag                                       <  2:128477/140‑1 (MQ=255)

                                                                                         atatGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCagag                                       <  2:1348167/140‑1 (MQ=255)

                                                                                           atGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCa                                     >  1:1590205/1‑140 (MQ=255)

                                                                                           atGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCa                                     <  2:567391/140‑1 (MQ=255)

                                                                                           atGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCa                                     >  1:275699/1‑140 (MQ=255)

                                                                                            tGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCAc                                    >  1:1728084/1‑140 (MQ=255)

                                                                                               aTCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGa                                 <  1:1347257/140‑1 (MQ=255)

                                                                                                 cAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGAcc                               >  1:362805/1‑140 (MQ=255)

                                                                                                 cAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGAcc                               <  1:766863/140‑1 (MQ=255)

                                                                                                 cAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGAcc                               >  1:197954/1‑140 (MQ=255)

                                                                                                   gctgctTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGc                             >  1:324097/1‑140 (MQ=255)

                                                                                                    ctgctTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCt                            >  2:1002352/1‑140 (MQ=255)

                                                                                                       ctTCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCtttt                         >  1:1337175/1‑140 (MQ=255)

                                                                                                         tCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTAt                       <  1:1377971/140‑1 (MQ=255)

                                                                                                         tCCCGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTAt                       <  1:650625/140‑1 (MQ=255)

                                                                                                            cGGTTCATCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTg                    <  1:489270/140‑1 (MQ=255)

                                                                                                                  aTCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGatatat              <  1:1749713/140‑1 (MQ=255)

                                                                                                                   tCCCCTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGAtatata             <  1:946412/140‑1 (MQ=255)

                                                                                                                    ccccTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATaa            >  1:865830/1‑140 (MQ=255)

                                                                                                                       cTTCATTTCATAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTCCCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATAATgg         <  2:1562063/140‑1 (MQ=255)

                                                                                                                        ttcatttcatAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATAATGGa        <  2:1590205/140‑1 (MQ=255)

                                                                                                                         tcatttcatAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATAATGGAc       >  1:1656283/1‑140 (MQ=255)

                                                                                                                         tcatttcatAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATAATGGAc       >  2:1074536/1‑140 (MQ=255)

                                                                                                                             ttcatAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTg                    <  1:1478135/123‑1 (MQ=255)

                                                                                                                               catAACCCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGat                  <  2:1174185/123‑1 (MQ=255)

GGTACAACATAAGGAACAGAAGTCTGGAATATACCATTTTCAATCCAGTAAAGGGTGTTTGCCCCTGGGCGTAAATTAAAGGCGGTGAGATATGCATCAGCTGCTTCCCGGTTCATCCCCTTCATTTCATAAACCTTGCCAAGCAACACATAATTTAGCCAGGACATTTCAAGATCAATGCCAGTATTTATCGCCTGGTAAGACTCATCTGTTTTACCTTTTACCAGAGCACTGACCGCTTTTATTTGATATATAATGGACAGGTT  >  NC_000913/4360427‑4360692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A