Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A11 F30 I1 R1 24 98.9 3381600 98.9% 3344402 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 398,629 C→A A253D (GCC→GAC)  yaiW → surface‑exposed outer membrane lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913398,6290CA100.0% 192.6 / NA 57A253D (GCC→GAC) yaiWsurface‑exposed outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (25/32);  total (25/32)

GTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAG  >  NC_000913/398498‑398763
                                                                                                                                   |                                                                                                                                      
gTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAgcggcg                                                                                                                                >  2:1034396/1‑140 (MQ=255)
 ttGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGt                                                                                                                               <  2:1506887/140‑1 (MQ=255)
 ttGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGt                                                                                                                               <  1:831597/140‑1 (MQ=255)
 ttGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAAAGCGGTCAGTAAGGACAGCGGCGt                                                                                                                               <  1:1393818/140‑1 (MQ=255)
      gTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGc                                                                                                                          <  1:759905/140‑1 (MQ=255)
        ttGGTACTTACCATTTACTGAATTATTCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTg                                                                                                                        <  1:1528806/140‑1 (MQ=255)
           gTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCa                                                                                                                     <  2:56947/140‑1 (MQ=255)
                     tttACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTg                                                                                                           >  2:1252704/1‑140 (MQ=255)
                     tttACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTg                                                                                                           >  1:864171/1‑140 (MQ=255)
                        aCTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACgg                                                                                                             >  2:637471/1‑135 (MQ=255)
                        aCTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACgg                                                                                                             <  1:637471/135‑1 (MQ=255)
                           gAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTaa                                                                                                     <  1:1424706/140‑1 (MQ=255)
                             aTTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAAtt                                                                                                   >  2:1127802/1‑140 (MQ=255)
                             aTTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAAtt                                                                                                   >  1:1240887/1‑140 (MQ=255)
                                 tCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGtt                                                                                               >  1:1031209/1‑140 (MQ=255)
                                 tCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGtt                                                                                               >  1:777548/1‑140 (MQ=255)
                                  cccGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTa                                                                                              <  1:1289384/140‑1 (MQ=255)
                                  cccGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTa                                                                                              <  2:944867/140‑1 (MQ=255)
                                  cccGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTa                                                                                              <  1:867084/140‑1 (MQ=255)
                                     gCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGa                                                                                           <  2:171767/140‑1 (MQ=255)
                                                   aCCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTa                                                                                      >  1:1599299/1‑131 (MQ=255)
                                                   aCCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTa                                                                                      <  2:1599299/131‑1 (MQ=255)
                                                    ccGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCgg                                                                            >  1:219221/1‑140 (MQ=255)
                                                    ccGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCgg                                                                            >  2:1355813/1‑140 (MQ=255)
                                                             cGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGa                                                                   >  2:822030/1‑140 (MQ=255)
                                                               ttttGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAAc                                                                 >  2:1225938/1‑140 (MQ=255)
                                                                 ttGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAAc                                                                      <  2:909029/133‑1 (MQ=255)
                                                                 ttGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAAc                                                                      >  1:909029/1‑133 (MQ=255)
                                                                  tGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTgg                                                              >  1:884414/1‑140 (MQ=255)
                                                                  tGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTgg                                                              >  2:923426/1‑140 (MQ=255)
                                                                  tGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTgg                                                              >  1:1243864/1‑140 (MQ=255)
                                                                       aTTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAAcg                                                         <  2:697749/140‑1 (MQ=255)
                                                                             aCGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCaaa                                                   <  1:1252704/140‑1 (MQ=255)
                                                                                   gctggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGc                                                             <  2:258927/124‑1 (MQ=255)
                                                                                   gctggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGc                                                             >  1:258927/1‑124 (MQ=255)
                                                                                   gctggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCt                                             >  1:1139545/1‑140 (MQ=255)
                                                                                      ggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCa                                          <  2:4989/140‑1 (MQ=255)
                                                                                      ggTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCa                                          <  2:1240887/140‑1 (MQ=255)
                                                                                       gTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCaa                                         <  1:1140084/140‑1 (MQ=255)
                                                                                           gCCAGCAGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACt                                     <  1:1127802/140‑1 (MQ=255)
                                                                                             cAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTgg                                   >  2:1229982/1‑140 (MQ=255)
                                                                                             cAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTgg                                   >  1:107398/1‑140 (MQ=255)
                                                                                             cAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGCTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACt                                     >  2:86255/1‑138 (MQ=255)
                                                                                             cAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGCTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACt                                     <  1:86255/138‑1 (MQ=255)
                                                                                               gCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTgggg                                 >  2:1066111/1‑140 (MQ=255)
                                                                                                  gTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATg                              >  1:457737/1‑140 (MQ=255)
                                                                                                     aTGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAATCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGc                           <  1:1034396/140‑1 (MQ=255)
                                                                                                     aTGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGc                           >  2:1215658/1‑140 (MQ=255)
                                                                                                     aTGCCGCGTTTCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGc                           <  2:107398/140‑1 (MQ=255)
                                                                                                             tttCAGAACGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGa                   >  2:1153940/1‑140 (MQ=255)
                                                                                                                   aaCGCGGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCg             <  1:1153940/140‑1 (MQ=255)
                                                                                                                   aaCGCGGTCAGTAAGGACAGCGGAGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCg             <  1:146562/140‑1 (MQ=255)
                                                                                                                     cgcgGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGcc           <  2:777548/140‑1 (MQ=255)
                                                                                                                     cgcgGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGcc           <  2:884414/140‑1 (MQ=255)
                                                                                                                     cgcgGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGcc           <  1:1215658/140‑1 (MQ=255)
                                                                                                                      gcgGTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCg          <  2:291561/140‑1 (MQ=255)
                                                                                                                        ggTCAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTc        >  1:928492/1‑140 (MQ=255)
                                                                                                                           cAGTAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGt     <  2:1243864/140‑1 (MQ=255)
                                                                                                                              tAAGGACAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTag  <  2:928492/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
GTTGTGGTTTGGTACTTACCATTTACTGAATTATCCCGCCAGTTATAGCGCACCGATATACCGTTTTGCTGATTTTAACGCTGGCTGGTACGCCAGCCGTAATGCCGCGTTTCAGAACGCGGTCAGTAAGGCCAGCGGCGTGAAGCTGGCACTGGACGGTGATTTAATTCGTTACGACAGTAAAGAACCCGGGAAAACCGAACTGGCAACGCGCAAACTGGCTGCAAAACTGGGGATGAGCGACAGCGAAATTCGCCGTCAGTTAG  >  NC_000913/398498‑398763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A