Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A30 F30 I1 R1 22 78.9 2706124 99.2% 2684475 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,448,530 G→T P28P (CCG→CCT)  chpS → ChpS antitoxin of the ChpB‑ChpS toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,448,5300GT98.2% 380.8 / ‑0.1 108P28P (CCG→CCT) chpSChpS antitoxin of the ChpB‑ChpS toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base T (61/45);  total (62/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 108 total aligned reads displayed.

CTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGT  >  NC_000913/4448402‑4448665
                                                                                                                                |                                                                                                                                       
cTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGt                                                                                                                              <  1:1012442/140‑1 (MQ=255)
cTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGt                                                                                                                              <  1:354085/140‑1 (MQ=255)
         cGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCa                                                                                                                     >  2:11038/1‑140 (MQ=255)
           tGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAg                                                                                                                   >  1:1035414/1‑140 (MQ=255)
           tGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAg                                                                                                                   <  2:444036/140‑1 (MQ=255)
           tGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAg                                                                                                                   >  2:784837/1‑140 (MQ=255)
            gAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGt                                                                                                                  <  1:868246/140‑1 (MQ=255)
                 ataCTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGca                                                                                                             <  1:784837/140‑1 (MQ=255)
                       tGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATc                                                                                                       >  2:700516/1‑140 (MQ=255)
                       tGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATc                                                                                                       >  2:843341/1‑140 (MQ=255)
                       tGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATc                                                                                                       >  1:1129286/1‑140 (MQ=255)
                       tGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATc                                                                                                       >  2:1234515/1‑140 (MQ=255)
                         tataACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCaa                                                                                                     <  1:11038/140‑1 (MQ=255)
                         tataACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCaa                                                                                                     >  2:232789/1‑140 (MQ=255)
                             aCTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGa                                                                                                 <  1:1276694/140‑1 (MQ=255)
                              cTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGAt                                                                                                >  2:39527/1‑140 (MQ=255)
                                 aaGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCt                                                                                             >  1:372120/1‑140 (MQ=255)
                                 aaGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCt                                                                                             >  1:890050/1‑140 (MQ=255)
                                 aaGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCt                                                                                             >  2:1053934/1‑140 (MQ=255)
                                   ggaggTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGa                                                                                           >  2:880803/1‑140 (MQ=255)
                                   ggaggTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGa                                                                                           >  1:728928/1‑140 (MQ=255)
                                   ggaggTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGa                                                                                           >  2:275239/1‑140 (MQ=255)
                                   ggaggTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGa                                                                                           <  1:1303630/140‑1 (MQ=255)
                                      ggTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGacac                                                                                        >  1:181123/1‑140 (MQ=255)
                                       gTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACAcc                                                                                       >  2:284401/1‑140 (MQ=255)
                                       gTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACAcc                                                                                       >  2:522937/1‑140 (MQ=255)
                                        tGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTg                                                                                                  >  2:125025/1‑128 (MQ=255)
                                        tGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTg                                                                                                  <  1:125025/128‑1 (MQ=255)
                                        tGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACAccc                                                                                      >  2:358397/1‑140 (MQ=255)
                                        tGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACAccc                                                                                      >  2:866343/1‑140 (MQ=255)
                                        tGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACAccc                                                                                      <  1:1053934/140‑1 (MQ=255)
                                        tGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACAccc                                                                                      >  1:716834/1‑140 (MQ=255)
                                        tGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACAccc                                                                                      >  2:103530/1‑140 (MQ=255)
                                        tGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACAccc                                                                                      >  1:180489/1‑140 (MQ=255)
                                         gCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCa                                                                                     >  1:1048209/1‑140 (MQ=255)
                                             aTGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCAtctc                                                                                 <  1:314033/140‑1 (MQ=255)
                                                 gTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAgg                                                                             <  1:233276/140‑1 (MQ=255)
                                                 gTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAgg                                                                             <  1:843341/140‑1 (MQ=255)
                                                  tATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGgc                                                                            >  1:1019742/1‑140 (MQ=255)
                                                      aCCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTa                                                                        <  1:275239/140‑1 (MQ=255)
                                                       ccATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTAc                                                                       >  2:672248/1‑140 (MQ=255)
                                                        cATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACt                                                                      <  1:39527/140‑1 (MQ=255)
                                                         aTAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTc                                                                     <  2:1035414/140‑1 (MQ=255)
                                                         aTAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTc                                                                     <  2:1198858/140‑1 (MQ=255)
                                                          tAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCg                                                                    >  1:403618/1‑140 (MQ=255)
                                                          tAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCg                                                                    >  1:860894/1‑140 (MQ=255)
                                                            aaaaGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCt                                                                  >  1:780700/1‑140 (MQ=255)
                                                                 aTGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTtgatg                                                             >  1:725334/1‑140 (MQ=255)
                                                                  tGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTtgatga                                                            >  1:689158/1‑140 (MQ=255)
                                                                  tGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTtgatga                                                            <  2:728928/140‑1 (MQ=255)
                                                                  tGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTtgatga                                                            >  1:260427/1‑140 (MQ=255)
                                                                  tGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTtgatga                                                            >  1:983265/1‑140 (MQ=255)
                                                                       gAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCTTCTCCAGGCGCTACTCGCTTGATGAActgc                                                       <  1:1419/140‑1 (MQ=255)
                                                                        aaCAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAActgct                                                      >  2:861256/1‑140 (MQ=255)
                                                                          cAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTgg                                                    <  2:716834/140‑1 (MQ=255)
                                                                           aGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGc                                                   >  1:977979/1‑140 (MQ=255)
                                                                           aGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACA‑CCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGca                                                  <  1:522937/140‑1 (MQ=255)
                                                                            gTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGca                                                  <  2:1129286/140‑1 (MQ=255)
                                                                            gTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGca                                                  <  1:103530/140‑1 (MQ=255)
                                                                            gTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGca                                                  <  1:1248372/140‑1 (MQ=255)
                                                                            gTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGca                                                  <  2:983265/140‑1 (MQ=255)
                                                                             tGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGcac                                                 >  2:529104/1‑140 (MQ=255)
                                                                             tGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGcac                                                 <  1:866343/140‑1 (MQ=255)
                                                                             tGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGcac                                                 <  2:403618/140‑1 (MQ=255)
                                                                                  gTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAgtgt                                            >  1:597361/1‑140 (MQ=255)
                                                                                    aTGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGa                                          >  1:263157/1‑140 (MQ=255)
                                                                                     tGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGc                                                   >  2:292318/1‑130 (MQ=255)
                                                                                       gTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTTGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGca                                                  >  1:292334/1‑129 (MQ=255)
                                                                                       gTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTTGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGca                                                  <  2:292334/129‑1 (MQ=255)
                                                                                       gTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAgt                                              >  1:25340/1‑133 (MQ=255)
                                                                                       gTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAgt                                              <  2:25340/133‑1 (MQ=255)
                                                                                       gTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACAt                                       >  2:1242124/1‑140 (MQ=255)
                                                                                       gTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACAt                                       >  1:1046838/1‑140 (MQ=255)
                                                                                       gTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACAt                                       >  1:588529/1‑140 (MQ=255)
                                                                                            tCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAAcg                                  >  2:579488/1‑140 (MQ=255)
                                                                                            tCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAAcg                                  >  1:1290657/1‑140 (MQ=255)
                                                                                            tCCCAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATAAAcg                                  >  1:16102/1‑140 (MQ=255)
                                                                                              ccAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAAcgcc                                <  1:591290/140‑1 (MQ=255)
                                                                                               cAATATCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAAcgccg                               <  1:579488/140‑1 (MQ=255)
                                                                                                  tatCGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCgg                            >  2:1332342/1‑140 (MQ=255)
                                                                                                     cGTAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAAc                         <  2:181123/140‑1 (MQ=255)
                                                                                                       tAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACtt                       >  1:1011055/1‑140 (MQ=255)
                                                                                                       tAATGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACtt                       >  2:750467/1‑140 (MQ=255)
                                                                                                         aTGAAAGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAg                     >  2:1134985/1‑140 (MQ=255)
                                                                                                            aaaGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGa                  <  2:372120/140‑1 (MQ=255)
                                                                                                              aGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGc                >  2:751576/1‑140 (MQ=255)
                                                                                                              aGAACTTAACTTACAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGc                >  2:608143/1‑140 (MQ=255)
                                                                                                                 acttaacttaCAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAgg             >  2:833319/1‑140 (MQ=255)
                                                                                                                 acttaacttaCAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAgg             <  1:997080/140‑1 (MQ=255)
                                                                                                                 acttaacttaCAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAgg             <  2:263157/140‑1 (MQ=255)
                                                                                                                 acttaacttaCAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAgg             <  2:220841/140‑1 (MQ=255)
                                                                                                                  cttaacttaCAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGa            >  1:835508/1‑140 (MQ=255)
                                                                                                                     aacttaCAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGt         >  2:152411/1‑140 (MQ=255)
                                                                                                                      acttaCAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTc        <  1:672248/140‑1 (MQ=255)
                                                                                                                      acttaCAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTc        <  2:780700/140‑1 (MQ=255)
                                                                                                                       cttaCAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCt       <  2:260427/140‑1 (MQ=255)
                                                                                                                       cttaCAGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCt       <  1:1242124/140‑1 (MQ=255)
                                                                                                                            aGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAGCGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGt  <  2:597361/140‑1 (MQ=255)
                                                                                                                            aGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGt  >  2:787639/1‑140 (MQ=255)
                                                                                                                            aGCCTGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGt  <  1:787639/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                                       
CTGTAGCACCGTGAGTTATACTTTGTATAACTTAAGGAGGTGCAGATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACAGCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATGAACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGT  >  NC_000913/4448402‑4448665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A