Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F30 I1 R1	25	75.3	2588958	98.8%	2557890	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,417,219	G→T	E90* (GAA→TAA)	yjfP →	carboxylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,417,219	0	G	T	100.0%	156.1 / NA	48	E90* (GAA→TAA)	yjfP	carboxylesterase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (22/26); total (22/26)

CTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTACCGC  >  NC_000913/4417083‑4417355

cTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAAt                                                                                                                                       >  2:293014/1‑140 (MQ=255)

cTATTTTGCCGTTGCGCTGGCACAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAAt                                                                                                                                       <  2:981844/140‑1 (MQ=255)

 tATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAAtt                                                                                                                                      <  2:136274/140‑1 (MQ=255)

  aTTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTc                                                                                                                                     >  2:219180/1‑140 (MQ=255)

          gTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAAACAAATCCGGCGAATCTTTCTACCAAGTATGCTATAATTCACTACttt                                                                                                                             >  2:637833/1‑140 (MQ=255)

                        ggCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAg                                                                                                               <  1:539571/140‑1 (MQ=255)

                        ggCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAg                                                                                                               <  1:684773/140‑1 (MQ=255)

                         gCTGGTTTGCGGGTGATCATGCCGGATGCGCTCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGc                                                                                                              >  1:21916/1‑140 (MQ=255)

                             gTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCgaa                                                                                                          <  2:338976/140‑1 (MQ=255)

                                   gggTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTTAATCAATTTTGGCAAATCTTGCTACAAAATATGCAGTAATTCACTCCTTTACGTGCGGCCATAGCCGAAGAAAAc                                                                                                    >  2:1227410/1‑140 (MQ=255)

                                   gggTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAAc                                                                                                    >  2:186783/1‑140 (MQ=255)

                                       gATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGgc                                                                                                <  1:1227410/140‑1 (MQ=255)

                                       gATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGgc                                                                                                <  1:540406/140‑1 (MQ=255)

                                        aTCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATTCAATCTGGCAAATCTTGCTACAAAATATGCAGTAATTTACTACTTTACCTGCGGCAATAGCCGAAGAAAACAGgct                                                                                               >  2:976320/1‑140 (MQ=255)

                                            tGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCtt                                                                                           <  2:540234/140‑1 (MQ=255)

                                             gCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtg                                                                                          >  1:656083/1‑140 (MQ=255)

                                                 gATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtgatga                                                                                      <  1:219180/140‑1 (MQ=255)

                                                 gATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTtgatga                                                                                      <  2:754114/140‑1 (MQ=255)

                                                  aTGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAAACTTGCTACAAAGTATGCAGTAATTCACTTCTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGTTGAc                                                                                     >  2:627814/1‑140 (MQ=255)

                                                   tGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGAcc                                                                                    >  1:928814/1‑140 (MQ=255)

                                                   tGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGAcc                                                                                    >  2:724557/1‑140 (MQ=255)

                                                        ccGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTg                                                                               >  2:83972/1‑140 (MQ=255)

                                                         cGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTgg                                                                              <  1:293014/140‑1 (MQ=255)

                                                                  tAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTg                                                                     >  1:549712/1‑140 (MQ=255)

                                                                  tAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTg                                                                     <  2:656083/140‑1 (MQ=255)

                                                                    gCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGgc                                                                   >  2:1037215/1‑140 (MQ=255)

                                                                    gCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGgc                                                                   >  1:88714/1‑140 (MQ=255)

                                                                       gTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGcgcg                                                                >  2:292245/1‑140 (MQ=255)

                                                                               ggtgACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATggg                                                        <  2:21916/140‑1 (MQ=255)

                                                                                gtgACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGgc                                                       <  1:903465/140‑1 (MQ=255)

                                                                                gtgACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGgc                                                       <  1:693190/140‑1 (MQ=255)

                                                                                   aCGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGcgcg                                                    >  2:293088/1‑140 (MQ=255)

                                                                                   aCGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGcgcg                                                    >  2:999777/1‑140 (MQ=255)

                                                                                       aGCGCGGCGGTTAAATCAATTTTGGCAAATCCTGCTACAAAGGAATGAGAAATTCACCACCTTACCGGCGGCCATAGCCGAAGAAAACTGGCTGCCTGTCGACCATCTGGCAGTCGGTGGCGCGGCCATGGGCGCGAAGa                                                >  2:1257113/1‑140 (MQ=255)

                                                                                       aGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGa                                                <  1:83972/140‑1 (MQ=255)

                                                                                        gcgcGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGa                                                >  1:911358/1‑139 (MQ=255)

                                                                                        gcgcGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGa                                                <  2:911358/139‑1 (MQ=255)

                                                                                         cgcgGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACg                                              >  2:183731/1‑140 (MQ=255)

                                                                                            ggcggTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCa                                           <  2:449164/140‑1 (MQ=255)

                                                                                            ggcggTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCa                                           <  2:549712/140‑1 (MQ=255)

                                                                                                          ttCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGc                             >  2:606528/1‑140 (MQ=255)

                                                                                                           tCTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCt                            >  1:173557/1‑140 (MQ=255)

                                                                                                            cTGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTc                           <  2:928814/140‑1 (MQ=255)

                                                                                                             tGGCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCg                          <  1:606528/140‑1 (MQ=255)

                                                                                                              ggCAAATCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGc                         >  1:151477/1‑140 (MQ=255)

                                                                                                                 aaaTCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCAc                      >  1:633320/1‑140 (MQ=255)

                                                                                                                  aaTCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCAcc                     <  1:293088/140‑1 (MQ=255)

                                                                                                                   aTCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCAccc                    >  2:780009/1‑140 (MQ=255)

                                                                                                                    tCTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCAcccc                   >  2:371708/1‑140 (MQ=255)

                                                                                                                     cTTGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCa                  <  1:1275375/140‑1 (MQ=255)

                                                                                                                       tGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACg                <  1:467378/140‑1 (MQ=255)

                                                                                                                       tGCTACAAAGTATGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACg                <  1:292245/140‑1 (MQ=255)

                                                                                                                                  aTGCAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTAc     >  2:468301/1‑140 (MQ=255)

                                                                                                                                     cAGTAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTACCGc  <  1:183731/140‑1 (MQ=255)

CTATTTTGCCGTTGCGCTGGCGCAGGCTGGTTTGCGGGTGATCATGCCGGATGCGCCCGATCACGGTAGCCGTTTTAGTGGTGACGCAGCGCGGCGGTTAAATCAATTCTGGCAAATCTTGCTACAAAGTATGCAGGAATTCACTACTTTACGTGCGGCAATAGCCGAAGAAAACTGGCTGCTTGATGACCGTCTGGCAGTCGGTGGCGCGTCGATGGGCGCGATGACGGCACTGGGGATTACCGCTCGCCACCCCACGGTGAGATGTACCGC  >  NC_000913/4417083‑4417355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A