breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 19,238 Δ558 bp IS1‑mediated [nhaR] [nhaR]
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 285,054 C→A T618T (ACC→ACA yagF → CP4‑6 prophage; D‑xylonate dehydratase
RA 527,765 T→C I62T (ATA→ACA)  ybbC → PF15631 family protein YbbC
RA 658,820 G→T intergenic (+2/‑127) ybeM → / → tatE deamidated glutathione amidase, N‑terminal fragment/twin arginine protein translocation system ‑ TatE protein
RA 726,385 C→A E218* (GAA→TAA)  kdpB ← K(+) transporting P‑type ATPase subunit KdpB
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,197,555 A→G F228S (TTC→TCC)  ymfE ← e14 prophage; uncharacterized protein YmfE
RA 1,287,614 A→G noncoding (9/85 nt) tyrT ← tRNA‑Tyr
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,081,905 G→T Q215K (CAA→AAA)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase DacD
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,625,164 A→T D17V (GAT→GTT)  ppx → exopolyphosphatase
RA 2,695,699 C→A intergenic (‑156/‑102) purL ← / → mltF phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,932,598 G→T S428I (AGC→ATC)  kup → K(+):H(+) symporter Kup
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,367,598 C→A E244* (GAG→TAG)  aspA ← aspartate ammonia‑lyase
RA 4,417,219 G→T E90* (GAA→TAA)  yjfP → carboxylesterase
RA 4,502,068 C→A intergenic (+479/+35) yjgZ → / ← insG KpLE2 phage‑like element; uncharacterized protein YjgZ/KpLE2 phage‑like element; IS4 putative transposase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102994 3104069 1076 19 [17] [18] 19 mutY mutY
* * ÷ NC_000913 3423787–3424231 3424531–3424239 9–745 20 [17] [18] 19 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374921 =NA (NA)65 (1.250) 25/190 0.8 NA intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = NA (NA)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)34 (0.660) 16/190 1.8 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375001 =NA (NA)53 (0.900) 24/216 1.2 NA noncoding (17/36 nt) other REP31b
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 375082 =NA (NA)56 (0.860) 20/238 1.9 NA intergenic (+201/‑377) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377372 = NA (NA)noncoding (37/37 nt) other REP32a
* ? NC_000913 375102 =NA (NA)40 (0.680) 15/216 2.3 NA noncoding (17/36 nt) other REP31d
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 1207790 =7 (0.090)87 (1.300) 55/244 0.1 95.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 3 (0.040)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078057 (0.090)51 (0.760) 34/244 0.7 91.7% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 3 (0.040)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)67 (0.910) 51/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =101 (1.340)149 (1.990) 64/274 0.1 74.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)89 (1.180) 50/276 0.3 96.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA