Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A42 F30 I1 R1	21	69.1	2346602	99.2%	2327829	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	801,298	G→T	Q180H (CAG→CAT)	ybhH →	putative isomerase YbhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	801,298	0	G	T	100.0%	162.1 / NA	46	Q180H (CAG→CAT)	ybhH	putative isomerase YbhH
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (18/28); total (18/28)

CAAACGCCAAATGGTGTTGTCGAGTACGAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAG  >  NC_000913/801161‑801422

cAAACGCCAAATGGTGTTGTCGAGTACGAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCatat                                                                                                                            >  2:263597/1‑140 (MQ=255)

   aCGCCAAATGGTGTTGTCGAGTACGAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAtattga                                                                                                                         <  1:401713/140‑1 (MQ=255)

    cGCCAAATGGTGTTGTCGAGTACGAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAtattgat                                                                                                                        <  1:192576/140‑1 (MQ=255)

              tgttgtCGAGTACGAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACg                                                                                                              >  1:814400/1‑140 (MQ=255)

              tgttgtCGAGTACGAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACg                                                                                                              <  1:626445/140‑1 (MQ=255)

                         aCGAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTg                                                                                                   <  2:749760/140‑1 (MQ=255)

                          cGAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGa                                                                                                  >  2:588617/1‑140 (MQ=255)

                           gAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGAc                                                                                                 <  2:973096/140‑1 (MQ=255)

                           gAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGAc                                                                                                 <  2:579727/140‑1 (MQ=255)

                           gAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGAc                                                                                                 <  2:1168332/140‑1 (MQ=255)

                             gggTAGCGCCAGAATTGACGGCGTACCGGTTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCt                                                                                               >  1:468170/1‑140 (MQ=255)

                                tAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTa                                                                                            <  1:306409/140‑1 (MQ=255)

                                tAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTa                                                                                            <  2:1157603/140‑1 (MQ=255)

                                 aGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTa                                                                                            <  1:1016312/139‑1 (MQ=255)

                                 aGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTa                                                                                            >  2:1016312/1‑139 (MQ=255)

                                  gcgcCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATc                                                                                          >  1:404297/1‑140 (MQ=255)

                                        gAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCg                                                                                                      >  2:803317/1‑122 (MQ=255)

                                        gAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCg                                                                                                      <  1:803317/122‑1 (MQ=255)

                                          aTTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGc                                                                                  <  1:263597/140‑1 (MQ=255)

                                             gACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGAt                                                                               >  1:1098665/1‑140 (MQ=255)

                                                 gCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCa                                                                           <  2:404297/140‑1 (MQ=255)

                                                   gTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAgt                                                                         >  1:915604/1‑140 (MQ=255)

                                                   gTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAgt                                                                         <  2:1098665/140‑1 (MQ=255)

                                                   gTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAgt                                                                         <  1:588617/140‑1 (MQ=255)

                                                       cGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAgtcgtc                                                                     <  1:244342/140‑1 (MQ=255)

                                                       cGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAgtcgtc                                                                     <  2:1063149/140‑1 (MQ=255)

                                                            aCTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCattat                                                                <  1:362050/140‑1 (MQ=255)

                                                              tGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAgt                                                                         >  2:871415/1‑129 (MQ=255)

                                                              tGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAgt                                                                         <  1:871415/129‑1 (MQ=255)

                                                               gCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTcc                                                             <  1:853970/140‑1 (MQ=255)

                                                                      cGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGaa                                                      <  1:408152/140‑1 (MQ=255)

                                                                      cGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTACCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGaa                                                      <  2:475637/140‑1 (MQ=255)

                                                                             gcTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATACCTgg                                               <  2:814400/140‑1 (MQ=255)

                                                                              cTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTggg                                              >  1:535670/1‑140 (MQ=255)

                                                                               tCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGa                                             <  2:468170/140‑1 (MQ=255)

                                                                                  cTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGaaaa                                          >  2:38340/1‑140 (MQ=255)

                                                                                   tttCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGaaaaa                                         <  1:802773/140‑1 (MQ=255)

                                                                                      ccTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAg                                      >  1:834109/1‑140 (MQ=255)

                                                                                           aTGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTAt                                 >  2:780760/1‑140 (MQ=255)

                                                                                             gCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGa                               >  2:961773/1‑140 (MQ=255)

                                                                                               cGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAAt                             >  1:27634/1‑140 (MQ=255)

                                                                                                    gAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACcg                        >  2:348147/1‑140 (MQ=255)

                                                                                                     aaCCAAAACCGGAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACcgg                       <  2:762494/140‑1 (MQ=255)

                                                                                                                gAAAAGTTTTCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGAt            >  2:186299/1‑140 (MQ=255)

                                                                                                                      ttttCCCGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGAc      <  1:778717/140‑1 (MQ=255)

                                                                                                                          cccGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAg  <  1:348147/140‑1 (MQ=255)

                                                                                                                          cccGACTGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAg  <  1:817000/140‑1 (MQ=255)

                                                                                                                                tGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATg           >  2:47377/1‑125 (MQ=255)

                                                                                                                                tGATAATCATATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATg           <  1:47377/125‑1 (MQ=255)

CAAACGCCAAATGGTGTTGTCGAGTACGAGGGTAGCGCCAGAATTGACGGCGTACCGGGTACTGCCGCACCGGTTGCGCTCACTTTCCTGAATGCCGCTGGAACCAAAACCGGAAAAGTTTTCCCGACTGATAATCAGATTGATTATTTTGACGATGTCCCGGTGACCTGTATCGATATGGCGATGCCAGTCGTCATTATTCCGGCTGAATATCTGGGAAAAACAGGTTATGAATTACCGGCGGAACTGGATGCCGACAAAG  >  NC_000913/801161‑801422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A