breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 801,298 G→T Q180H (CAG→CAT ybhH → putative isomerase YbhH
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,938,954 G→T A436S (GCA→TCA)  pykA → pyruvate kinase II
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,319,376 G→T R167R (CGC→CGA rcsC ← sensory histidine kinase RcsC
RA 2,479,358 C→A H53N (CAT→AAT)  dsdA → D‑serine ammonia‑lyase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,481,549 C→A G87G (GGC→GGA yheS → ABC transporter ATP‑binding protein YheS
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,974,662 C→A L66M (CTG→ATG)  rffC → dTDP‑4‑amino‑4,6‑dideoxy‑D‑galactose acyltransferase
RA 4,032,143 T→G L328R (CTT→CGT)  pepQ → Xaa‑Pro dipeptidase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196241 1211428 15188 19 [16] [16] 20 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102949 3104060 1112 19 [16] [17] 18 [mutY] [mutY]
* * ÷ NC_000913 3423781–3424234 3424523–3424238 5–743 18 [17] [11] 18 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 3363001 =108 (1.560)109 (1.580) 62/274 0.1 66.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)78 (1.130) 49/276 0.2 97.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA