Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A17 F30 I1 R1 23 30.3 1021886 99.1% 1012689 139.4

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 703,519 G→A A31A (GCC→GCT)  nagB ← glucosamine‑6‑phosphate deaminase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913703,5190GA100.0% 58.5 / NA 18A31A (GCC→GCT) nagBglucosamine‑6‑phosphate deaminase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/10);  total (8/10)

GACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCGGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCATTATTCACCTCAATAAGTAAAATGTAAGCCGTTGGCGG  >  NC_000913/703383‑703648
                                                                                                                                        |                                                                                                                                 
gACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCa                                                                                                                                >  2:450077/1‑140 (MQ=255)
 aCCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATcagcag                                                                                                                               <  2:286653/140‑1 (MQ=255)
           tCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGaa                                                                                                                     <  1:428570/140‑1 (MQ=255)
                              cAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACg                                                                                                  >  2:450185/1‑140 (MQ=255)
                                               aCCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCa                                                                                 >  2:336271/1‑140 (MQ=255)
                                                        gCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGAc                                                                        <  1:361028/140‑1 (MQ=255)
                                                        gCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGAc                                                                        <  1:450185/140‑1 (MQ=255)
                                                              tGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTTCCGACCTGttt                                                                  >  2:117258/1‑139 (MQ=255)
                                                              tGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTc                                                                  >  1:142643/1‑140 (MQ=255)
                                                                   ttCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCgg                                                             >  1:506886/1‑140 (MQ=255)
                                                                           aCGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAATGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAgg                                                     <  1:217257/140‑1 (MQ=255)
                                                                           aCGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAgg                                                     <  2:506886/140‑1 (MQ=255)
                                                                                ttATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGAt                                                <  1:230260/140‑1 (MQ=255)
                                                                                 tataGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGGCCTGTTCAGCGGTAGTCAGGGGGATc                                               <  1:465006/140‑1 (MQ=255)
                                                                                   taGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAg                                             <  1:450077/140‑1 (MQ=255)
                                                                                      gtggtCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGtct                                          >  2:40542/1‑140 (MQ=255)
                                                                                         gtCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCat                                       >  2:459367/1‑140 (MQ=255)
                                                                                               ggCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCATTATTCa                                 <  1:383085/140‑1 (MQ=255)
                                                                                                                          aCAAACGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCATTATTCACCTCAATAAGTAAAATGTAAGCCGTTg      <  2:142643/140‑1 (MQ=255)
                                                                                                                              aCGGACGATCAGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCATTATTCACCTCAATAAGTAAAATGTAAGCCGTTGGCgg  >  1:172448/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                 
GACCGACATATTCGTCCATGTTGAAGGTGACAACGTGCTTAAAGCTGACCTGGCCTGCTTTATGCATTTCGACTAACGCTTTATAGGTGGTCATCGGCGTGCCGCCAGTCGGCAGGCCCAGTACAAACGGACGATCGGCAGTCGGTTTGAACGCATTGATACGATTGACGATATGGCGAGCAGCCCATTTGCCGACCTGTTCAGCGGTAGTCAGGGGGATCAGTCTCATTATTCACCTCAATAAGTAAAATGTAAGCCGTTGGCGG  >  NC_000913/703383‑703648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A