Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F28 I1 R1	116	49.4	1663006	99.2%	1649701	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,351,634	T→C	K464K (AAA→AAG)	arcB ←	sensory histidine kinase ArcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,351,634	0	T	C	100.0%	131.7 / NA	41	K464K (AAA→AAG)	arcB	sensory histidine kinase ArcB
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (17/24); total (17/24)

CGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAGCGCACGCGCACGG  >  NC_000913/3351505‑3351767

cGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCAt                                                                                                                             <  2:437738/140‑1 (MQ=255)

cGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCAt                                                                                                                             >  2:143767/1‑140 (MQ=255)

 gACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATg                                                                                                                            >  2:814097/1‑140 (MQ=255)

 gACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATg                                                                                                                            >  2:755538/1‑140 (MQ=255)

 gACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATg                                                                                                                            >  2:289954/1‑140 (MQ=255)

   cGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGAc                                                                                                                          <  1:814097/140‑1 (MQ=255)

   cGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGAc                                                                                                                          <  1:426940/140‑1 (MQ=255)

            gATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTACCCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATCCCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCa                                                                                                                 <  2:795172/140‑1 (MQ=255)

            gATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCa                                                                                                                 <  1:95508/140‑1 (MQ=255)

            gATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCa                                                                                                                 <  2:74842/140‑1 (MQ=255)

            gATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCa                                                                                                                 <  1:234344/140‑1 (MQ=255)

            gATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCa                                                                                                                 <  1:289954/140‑1 (MQ=255)

              tCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCAcc                                                                                                               <  1:314185/140‑1 (MQ=255)

                gTCAACTTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATCCCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTg                                                                                                             <  2:551605/140‑1 (MQ=255)

                gTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGcc                                                                                                                               <  2:658014/122‑1 (MQ=255)

                gTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGcc                                                                                                                               >  1:658014/1‑122 (MQ=255)

                gTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTg                                                                                                             >  1:585768/1‑140 (MQ=255)

                gTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTg                                                                                                             >  2:273667/1‑140 (MQ=255)

                       tAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATAc                                                                                                      >  1:312510/1‑140 (MQ=255)

                              tGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGa                                                                                               <  1:143767/140‑1 (MQ=255)

                               gAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGaa                                                                                              >  1:730042/1‑140 (MQ=255)

                                      tGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTa                                                                                       >  1:414541/1‑140 (MQ=255)

                                        cccTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATc                                                                                     <  1:755538/140‑1 (MQ=255)

                                               cGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGa                                                                                               <  1:314958/123‑1 (MQ=255)

                                               cGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGa                                                                                               >  2:314958/1‑123 (MQ=255)

                                               cGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCa                                                                              <  1:273667/140‑1 (MQ=255)

                                                    gTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTg                                                                         <  1:543861/140‑1 (MQ=255)

                                                                   ccgccCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATAcc                                                          >  2:423673/1‑140 (MQ=255)

                                                                      cccATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCaga                                                       >  1:797465/1‑140 (MQ=255)

                                                                       ccATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCagag                                                      <  1:423673/140‑1 (MQ=255)

                                                                         atatTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTc                                                    <  2:312510/140‑1 (MQ=255)

                                                                            tttttCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTc                                                 <  2:797465/140‑1 (MQ=255)

                                                                                     aGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCTCTTCaaa                                        <  2:585768/140‑1 (MQ=255)

                                                                                         gacgaGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATgc                                    <  2:197763/140‑1 (MQ=255)

                                                                                               aaaCGGCCAGACCAATCCCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCata                              <  2:552078/140‑1 (MQ=255)

                                                                                                        gACCAATACCGGTGCCGGTGGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCa                     >  2:30269/1‑140 (MQ=255)

                                                                                                                   gtgccggtgGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAgcgcac          >  1:502543/1‑140 (MQ=255)

                                                                                                                     gccggtgGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAgcgcacgc        <  2:730042/140‑1 (MQ=255)

                                                                                                                      ccggtgGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCCGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAgcgcacgcg       <  1:649581/140‑1 (MQ=255)

                                                                                                                        ggtgGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAgcgcacgcgca     >  2:775881/1‑140 (MQ=255)

                                                                                                                         gtgGCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAgcgcacgcgcac    <  1:54206/140‑1 (MQ=255)

                                                                                                                           ggCAGGCTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAGCGCACGCGCACgg  >  1:419810/1‑140 (MQ=255)

CGACGGTGCGTGGATCGTCAACGTAAAGGTTGAACCTTTGCCCTGTTCGCTGGTAACCGTAATATCGCCGCCCATATTTTTCGCCAGACGACGAGAAACGGCCAGACCAATACCGGTGCCGGTGGCAGGTTTACCGCCATGACTGTCTTTCACCTGGTAATACATGGCGAAAATTTTATCCAGCTCATCCTGCGGAATGCCGATACCAGAGTCTTCCACTTCAAAATGCAGCATATCGCCTTCATCGTAGCGCACGCGCACGG  >  NC_000913/3351505‑3351767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A