Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A7 F28 I1 R1	252	48.5	1661298	98.8%	1641362	138.6

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,624,281	A→G	L170P (CTG→CCG)	ytjB ←	protein Smp

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,624,281	0	A	G	100.0%	127.2 / NA	39	L170P (CTG→CCG)	ytjB	protein Smp
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/19); total (20/19)

TCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTT  >  NC_000913/4624145‑4624417

tCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGagcggc                                                                                                                                        >  1:319551/1‑139 (MQ=255)

   ctctTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACa                                                                                                                                    >  1:428932/1‑140 (MQ=255)

    tctTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACAt                                                                                                                                   <  2:744/140‑1 (MQ=255)

        ttttCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAgg                                                                                                                               >  1:228870/1‑140 (MQ=255)

              cGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTaaa                                                                                                                         >  2:517646/1‑140 (MQ=255)

                cTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAAt                                                                                                                       >  1:164713/1‑140 (MQ=255)

                     ttcctcttccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAg                                                                                                                  <  1:442858/140‑1 (MQ=255)

                       cctcttccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAgtg                                                                                                                <  2:164713/140‑1 (MQ=255)

                           ttccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGGGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGgtgt                                                                                                            >  1:546137/1‑140 (MQ=255)

                             ccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTa                                                                                                          >  2:565264/1‑140 (MQ=255)

                             ccGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGAGAGAGCGGCAATATCAGGCGTAAAATGTTAGTGGTGTTa                                                                                                          >  1:391081/1‑140 (MQ=255)

                               ggCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGGGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATc                                                                                                        >  1:807741/1‑140 (MQ=255)

                                              gCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTc                                                                                         <  2:282189/140‑1 (MQ=255)

                                              gCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGGCAGCACTACACCGATTTCCAGTGAGAGCGGCAACCTCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTc                                                                                         >  1:564429/1‑140 (MQ=255)

                                               cGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCg                                                                                        <  1:565264/140‑1 (MQ=255)

                                                    aaCAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGc                                                                                   <  2:428932/140‑1 (MQ=255)

                                                       aGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAg                                                                                <  1:529389/140‑1 (MQ=255)

                                                            gggCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTAt                                                                           >  1:709095/1‑140 (MQ=255)

                                                               cGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATggg                                                                        <  2:228870/140‑1 (MQ=255)

                                                                   tgctgcCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGc                                                                                   >  1:247334/1‑125 (MQ=255)

                                                                   tgctgcCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGc                                                                                   <  2:247334/125‑1 (MQ=255)

                                                                            cGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAg                                                           >  1:644451/1‑140 (MQ=255)

                                                                                          cTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGa                                             <  2:546137/140‑1 (MQ=255)

                                                                                             tAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCg                                          >  2:401643/1‑140 (MQ=255)

                                                                                               gcagcGTGCGGGTCAGCACTACACCGATTGCCAGAGAGAGCGGCAATATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGt                                        <  2:391081/140‑1 (MQ=255)

                                                                                                       cGGGTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGttttt                                >  1:598436/1‑140 (MQ=255)

                                                                                                         ggTCAGCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTAc                              <  1:698875/140‑1 (MQ=255)

                                                                                                              gCACTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCa                         <  2:807741/140‑1 (MQ=255)

                                                                                                                 cTACACCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAAtt                      <  2:644451/140‑1 (MQ=255)

                                                                                                                    cacCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGc                   >  1:552245/1‑140 (MQ=255)

                                                                                                                    cacCGATTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGc                   >  1:318083/1‑140 (MQ=255)

                                                                                                                         aTTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGAc              >  1:782547/1‑140 (MQ=255)

                                                                                                                         aTTGCCAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGAc              >  2:564406/1‑140 (MQ=255)

                                                                                                                              cAGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATct         >  2:623552/1‑140 (MQ=255)

                                                                                                                               aGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctg        >  2:20916/1‑140 (MQ=255)

                                                                                                                               aGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctg        <  2:645376/140‑1 (MQ=255)

                                                                                                                               aGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctg        <  2:709095/140‑1 (MQ=255)

                                                                                                                               aGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctg        <  1:20916/140‑1 (MQ=255)

                                                                                                                               aGTGAGAGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATAGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctg        <  1:517646/140‑1 (MQ=255)

                                                                                                                                  gagaGCGGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATctgctg     <  1:401643/140‑1 (MQ=255)

                                                                                                                                     agcggcAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGtt  >  2:772466/1‑140 (MQ=255)

TCACTCTTTTTTCTCGCTTTCTTCCTCTTCCGGCACCGGTTTGCTGGCGGTTAACAGGAAGGGCGATTGCTGCCAGCGGGTGCGTTTACCCTGTAGCAGCGTGCGGGTCAGCACTACACCGATTGCCAGTGAGAGCAGCAACATCAGGCGTAAAATGTTAGTGGTGTTATCCACCTGTTGGGCTTCGGTGGCGAGCGTATGGGTGTCGAGTGTCAGGCGCAGATAGCCGAGCGGTCCGTTTTTACCCGCAATTGGCTCGACAATCTGCTGGTT  >  NC_000913/4624145‑4624417

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A