breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 21,427 A→G I7M (ATA→ATG ribF → bifunctional riboflavin kinase/FMN adenylyltransferase
RA 73,675 C→T D283N (GAT→AAT)  thiP ← thiamine ABC transporter membrane subunit
RA 111,472 A→G intergenic (+39/+177) mutT → / ← yacG 8‑oxo‑dGTP diphosphatase/DNA gyrase inhibitor YacG
RA 134,887 T→C K232K (AAA→AAG speD ← S‑adenosylmethionine decarboxylase proenzyme
RA 140,015 Δ1 bp coding (1211/2391 nt) gcd ← quinoprotein glucose dehydrogenase
RA 154,247 T→G T394P (ACG→CCG)  htrE ← putative fimbrial usher protein HtrE
RA 154,673 A→G S252P (TCT→CCT)  htrE ← putative fimbrial usher protein HtrE
RA 163,921 A→G D606G (GAC→GGC)  hrpB → putative ATP‑dependent RNA helicase HrpB
RA 168,708 A→G T409A (ACC→GCC)  fhuA → ferrichrome outer membrane transporter/phage receptor
RA 210,867 A→G T397A (ACC→GCC)  ldcC → lysine decarboxylase 2
RA 256,653 A→G T43A (ACC→GCC)  frsA → fermentation‑respiration switch protein
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 265,136 A→G V24A (GTC→GCC)  yafX ← CP4‑6 prophage; protein YafX
RA 303,093 (G)6→7 coding (103/615 nt) yagU → inner membrane protein that contributes to acid resistance
RA 306,891 A→G G814G (GGT→GGC ecpC ← putative fimbrial usher protein EcpC
RA 321,642 A→G D12G (GAC→GGC)  ykgE → putative lactate utilization oxidoreductase YkgE
RA 324,899 T→C T156A (ACA→GCA)  ykgH ← uncharacterized protein YkgH
RA 331,300 A→G Y613C (TAC→TGC)  betT → choline:H(+) symporter
RA 342,567 Δ1 bp intergenic (+60/‑317) yahJ → / → yahK putative deaminase with metallo‑dependent hydrolase domain/aldehyde reductase, NADPH‑dependent
RA 343,422 T→G V180G (GTG→GGG)  yahK → aldehyde reductase, NADPH‑dependent
RA 345,601 (G)5→6 coding (198/246 nt) yahM → uncharacterized protein YahM
RA 347,702 T→C T248A (ACG→GCG)  prpR ← DNA‑binding transcriptional dual regulator PrpR
RA 349,785 A→G intergenic (+213/‑227) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 355,098 T→C G59G (GGT→GGC codB → cytosine transporter
RA 409,791 A→G S77P (TCT→CCT)  rdgC ← nucleoid‑associated protein RdgC
RA 417,596 C→T P152L (CCG→CTG)  phoB → DNA‑binding transcriptional dual regulator PhoB
RA 428,460 A→G I272V (ATT→GTT)  secD → Sec translocon accessory complex subunit SecD
RA 448,202 A→G G153G (GGT→GGC cyoC ← cytochrome bo3 ubiquinol oxidase subunit 3
RA 455,861 T→C R243R (CGT→CGC tig → trigger factor
RA 503,187 C→T A18T (GCC→ACC)  ybaL ← putative transporter YbaL
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 558,618 A→G N136S (AAT→AGT)  sfmA → putative fimbrial protein SfmA
RA 562,090 A→G S798S (TCA→TCG sfmD → putative fimbrial usher protein SfmD
RA 587,362 A→G V207A (GTC→GCC)  ybcH ← DUF4434 domain‑containing protein YbcH
RA 589,388 A→G Y523H (TAT→CAT)  nfrA ← bacteriophage N4 receptor, outer membrane protein
RA 591,410 T→G D590A (GAC→GCC)  nfrB ← bacteriophage N4 receptor, inner membrane subunit
RA 601,896 G→A intergenic (+39/‑63) cusA → / → pheP copper/silver export system RND permease/phenylalanine:H(+) symporter PheP
RA 605,944 T→C intergenic (‑58/+7) ybdF ← / ← ybdJ PF04237 family protein YbdF/DUF1158 domain‑containing protein YbdJ
RA 609,518 A→G F188L (TTT→CTT)  entD ← phosphopantetheinyl transferase EntD
RA 634,722 A→G A379A (GCA→GCG ybdL → methionine‑oxo‑acid transaminase, PLP‑dependent
RA 646,209 A→G R124R (CGT→CGC citT ← citrate:succinate antiporter
RA 656,758 A→G T68A (ACC→GCC)  pagP → Lipid IVA palmitoyltransferase
RA 679,763 G→A A86T (GCA→ACA)  ybeU → DUF1266 domain‑containing protein YbeU
RA 690,049 T→C Q278R (CAA→CGA)  lnt ← apolipoprotein N‑acyltransferase
RA 696,592 T→C noncoding (25/75 nt) glnV ← tRNA‑Gln
RA 709,870 T→C S27S (AGT→AGC chiQ → lipoprotein ChiQ
RA 731,035 A→T I485M (ATA→TTG)  rhsC → rhs element protein RhsC
RA 731,037 A→G I485M (ATA→TTG rhsC → rhs element protein RhsC
RA 731,455 T→C C625R (TGT→CGT)  rhsC → rhs element protein RhsC
RA 731,472 T→G G630G (GGT→GGG rhsC → rhs element protein RhsC
RA 743,934 (A)5→4 coding (342/657 nt) pxpB → 5‑oxoprolinase component B
RA 800,419 T→C T53A (ACA→GCA)  ybhD ← putative DNA‑binding transcriptional regulator YbhD
RA 810,031 A→G T230A (ACG→GCG)  bioB → biotin synthase
RA 813,338 A→G intergenic (+391/‑188) bioD → / → uvrB dethiobiotin synthetase/excision nuclease subunit B
RA 823,294 A→G V149A (GTA→GCA)  clsB ← cardiolipin synthase B
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 902,309 A→G V227A (GTG→GCG)  artI ← putative ABC transporter periplasmic binding protein ArtI
RA 925,969 A→G noncoding (3/88 nt) serW ← tRNA‑Ser
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 961,913 A→G intergenic (+29/‑82) cmk → / → rpsA cytidylate kinase/30S ribosomal subunit protein S1
RA 991,561 T→C F314L (TTC→CTC)  pepN → aminopeptidase N
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,015,783 A→G G23G (GGA→GGG rmf → ribosome modulation factor
RA 1,018,976 (A)8→7 intergenic (+39/+37) matP → / ← ompA macrodomain Ter protein/outer membrane porin A
RA 1,032,104 T→C intergenic (‑392/‑35) serT ← / → hyaA tRNA‑Ser/hydrogenase 1 small subunit
RA 1,074,846 A→G Q202R (CAG→CGG)  rutR → DNA‑binding transcriptional dual regulator RutR
RA 1,131,195 A→G T60A (ACC→GCC)  flgB → flagellar basal‑body rod protein FlgB
RA 1,139,597 T→C V407A (GTT→GCT)  flgK → flagellar hook‑filament junction protein 1
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,151,919 T→C intergenic (+68/‑20) acpP → / → fabF acyl carrier protein/beta‑ketoacyl‑[acyl carrier protein] synthase II
RA 1,158,486 A→G V206V (GTA→GTG ptsG → glucose‑specific PTS enzyme IIBC component
RA 1,168,979 (A)6→5 intergenic (‑147/‑94) comR ← / → bhsA DNA‑binding transcriptional repressor ComR/DUF1471 domain‑containing multiple stress resistance outer membrane protein BhsA
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,282,908 T→C G1015G (GGT→GGC narG → nitrate reductase A subunit alpha
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,324,648 T→C V184A (GTT→GCT)  yciO → putative RNA‑binding protein YciO
RA 1,329,681 T→C V117A (GTG→GCG)  sohB → S49 peptidase family protein
RA 1,336,334 T→C S168S (AGT→AGC acnA → aconitate hydratase 1
RA 1,336,381 A→G N184S (AAC→AGC)  acnA → aconitate hydratase 1
RA 1,348,051 G→A L288F (CTC→TTC)  rnb ← RNase II
RA 1,351,696 T→C K22R (AAG→AGG)  ycjD ← DUF559 domain‑containing protein YcjD
RA 1,358,269 C→T A307T (GCT→ACT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
RA 1,373,029 A→G N374S (AAT→AGT)  ycjN → putative ABC transporter periplasmic binding protein YcjN
RA 1,376,293 C→T A87V (GCG→GTG)  ycjR → putative ketohexose‑3‑epimerase YcjR
RA 1,396,107 T→C F11S (TTC→TCC)  insH‑4 → IS5 transposase and trans‑activator
RA 1,420,357 T→G intergenic (‑116/‑8) racR ← / → ydaS Rac prophage; DNA‑binding transcriptional repressor RacR/Rac prophage; toxin YdaS
RA 1,430,254 A→G R402R (AGA→AGG stfR → Rac prophage; putative membrane protein
RA 1,445,992 A→G Y38C (TAC→TGC)  ydbL → DUF1318 domain‑containing protein YdbL
RA 1,449,064 (T)7→6 intergenic (+46/+12) feaB → / ← tynA phenylacetaldehyde dehydrogenase/copper‑containing amine oxidase
RA 1,488,752 T→C V174A (GTC→GCC)  aldA → aldehyde dehydrogenase A
RA 1,519,516 (G)5→6 coding (490/1038 nt) curA → NADPH‑dependent curcumin/dihydrocurcumin reductase
RA 1,526,028 A→G intergenic (‑48/‑219) ansP ← / → yncG L‑asparagine transporter/putative glutathione S‑transferase YncG
RA 1,541,275 T→G N439T (AAT→ACT)  narZ ← nitrate reductase Z subunit alpha
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,542,454 A→G V46A (GTG→GCG)  narZ ← nitrate reductase Z subunit alpha
RA 1,542,644 A→G intergenic (‑54/+28) narZ ← / ← narU nitrate reductase Z subunit alpha/nitrate/nitrite transporter NarU
RA 1,546,963 A→G S69S (AGT→AGC yddG ← aromatic amino acid exporter
RA 1,599,454 G→A G252G (GGC→GGT lsrK ← autoinducer‑2 kinase
RA 1,617,606 T→C S193S (AGT→AGC ydeA → L‑arabinose exporter
RA 1,632,327 T→C intergenic (‑42/‑563) ydfJ ← / → ynfT putative transporter YdfJ/Qin prophage; protein YnfT
RA 1,634,642 C→T G82S (GGC→AGC)  tfaQ ← Qin prophage; putative tail fiber assembly protein TfaQ
RA 1,635,861 T→C I169M (ATA→ATG nohA ← Qin prophage; putative prophage DNA‑packaging protein NohA
RA 1,641,656 A→G intergenic (‑102/‑199) cspB ← / → cspF Qin prophage; cold shock‑like protein CspB/Qin prophage; cold shock protein CspF
RA 1,666,097 A→G A261A (GCA→GCG clcB → putative chloride:H(+) antiporter ClcB
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,690,148 T→C G99G (GGT→GGC ydgA → conserved protein YdgA
RA 1,708,437 G→T A507S (GCT→TCT)  rsxC → SoxR [2Fe‑2S] reducing system protein RsxC
RA 1,714,096 T→C V443A (GTG→GCG)  dtpA → dipeptide/tripeptide:H(+) symporter DtpA
RA 1,721,339 T→C W26R (TGG→CGG)  ydhJ → putative membrane fusion protein YdhJ
RA 1,736,876 T→C T139A (ACC→GCC)  ydhP ← putative transporter YdhP
RA 1,743,648 G→A L64L (CTG→CTA mdtK → multidrug efflux pump MdtK
RA 1,758,213 T→C G171G (GGA→GGG ldtE ← L,D‑transpeptidase LdtE
RA 1,777,538 A→G T115A (ACG→GCG)  ydiO → putative acyl‑CoA dehydrogenase
RA 1,830,377 T→C D130G (GAC→GGC)  astA ← arginine N‑succinyltransferase
RA 1,837,853 T→C G214G (GGT→GGC ynjC → putative ABC transporter membrane subunit YnjC
RA 1,841,011 T→C A131A (GCA→GCG ynjF ← putative phosphatidyl transferase, inner membrane protein
RA 1,871,832 (G)6→7 coding (1448/1476 nt) cdgI → putative c‑di‑GMP binding protein CdgI
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
RA 1,875,056 (C)6→7 coding (521/822 nt) nimR ← DNA‑binding transcriptional repressor NimR
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,911,616 T→C G447G (GGT→GGC yebQ → putative transporter YebQ
RA 1,923,526 T→C T61A (ACC→GCC)  yebY ← DUF2511 domain‑containing protein YebY
RA 1,923,946 (G)6→7 coding (646/873 nt) yebZ ← putative inner membrane protein
RA 1,940,521 T→C Q372R (CAG→CGG)  mepM ← peptidoglycan DD‑endopeptidase MepM
RA 1,948,807 G→A N572N (AAC→AAT aspS ← aspartate‑‑tRNA ligase
RA 1,949,996 T→C Y176C (TAC→TGC)  aspS ← aspartate‑‑tRNA ligase
RA 1,969,994 T→C T331A (ACA→GCA)  tap ← methyl‑accepting chemotaxis protein Tap
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,012,563 (G)6→7 pseudogene (62/279 nt) intG → putative defective phage integrase
RA 2,014,971 A→G Q31R (CAG→CGG)  fliG → flagellar motor switch protein FliG
RA 2,065,765 A→G intergenic (‑1/+540) cobU ← / ← insH‑6 cobinamide‑P guanylyltransferase/cobinamide kinase/CP4‑44 prophage; IS5 transposase and trans‑activator
RA 2,073,659 T→C R707R (CGT→CGC flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,112,219 T→C V281V (GTA→GTG rfbB ← dTDP‑glucose 4,6‑dehydratase 1
RA 2,117,585 Δ1 bp coding (820/1281 nt) wcaK ← putative colanic acid biosynthesis pyruvyl transferase
RA 2,122,017 T→C E321E (GAA→GAG cpsG ← phosphomannomutase
RA 2,139,747 T→C intergenic (+262/+12) yegH → / ← asmA inner membrane protein YegH/putative assembly protein AsmA
RA 2,143,407 T→C F48L (TTC→CTC)  dgcE → putative diguanylate cyclase DgcE
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,177,101 A→G V35A (GTA→GCA)  gatY ← tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY
RA 2,185,875 (G)7→8 intergenic (‑79/‑42) rcnR ← / → rcnA DNA‑binding transcriptional repressor RcnR/Ni(2(+))/Co(2(+)) exporter
RA 2,230,404 (A)7→6 intergenic (‑19/‑220) dusC ← / → yohJ tRNA‑dihydrouridine(16) synthase/PF03788 family membrane protein YohJ
RA 2,236,296 T→C C344C (TGT→TGC preA → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreA
RA 2,246,731 A→G C13C (TGT→TGC cirA ← ferric dihyroxybenzoylserine outer membrane transporter
RA 2,249,929 T→C G71G (GGT→GGC yeiH → conserved inner membrane protein YeiH
RA 2,251,878 G→A C60Y (TGC→TAC)  yeiI → putative sugar kinase YeiI
RA 2,278,414 G→A intergenic (‑177/+156) yejG ← / ← bcr protein YejG/multidrug efflux pump Bcr
RA 2,283,721 T→C D76G (GAC→GGC)  yejK ← nucleoid‑associated protein YejK
RA 2,284,565 C→T L64L (CTG→TTG)  yejM → putative cardiolipin transport protein
RA 2,305,788 T→C M323V (ATG→GTG)  mqo ← malate:quinone oxidoreductase
RA 2,313,600 T→C V38A (GTA→GCA)  rcsD → RcsD phosphotransferase
RA 2,324,502 T→C S132P (TCG→CCG)  atoA → acetyl‑CoA:acetoacetyl‑CoA transferase subunit beta
RA 2,401,970 T→C H28R (CAC→CGC)  nuoE ← NADH:quinone oxidoreductase subunit E
RA 2,437,591 T→C A87A (GCA→GCG pdxB ← erythronate‑4‑phosphate dehydrogenase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,457,031 G→A A710V (GCA→GTA)  fadJ ← 3‑hydroxyacyl‑CoA dehydrogenase FadJ
RA 2,462,889 (C)9→10 intergenic (+243/‑123) fadL → / → yfdF long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor/protein YfdF
RA 2,492,281 A→G V325A (GTG→GCG)  frc ← formyl‑CoA transferase
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,530,088 A→T I30N (ATT→AAT)  ligA ← DNA ligase
RA 2,597,353 A→G V89A (GTT→GCT)  purC ← phosphoribosylaminoimidazole‑succinocarboxamide synthase
RA 2,612,137 T→C Y80H (TAT→CAT)  hyfR → DNA‑binding transcriptional activator HyfR
RA 2,649,467 G→A R941C (CGT→TGT)  yfhM ← alpha2‑macroglobulin
RA 2,660,213 T→C N26S (AAC→AGC)  iscU ← scaffold protein for iron‑sulfur cluster assembly
RA 2,692,772 T→C E924E (GAA→GAG purL ← phosphoribosylformylglycinamide synthetase
RA 2,698,057 A→G V165A (GTC→GCC)  pgpC ← phosphatidylglycerophosphatase C
RA 2,699,413 G→A A219T (GCC→ACC)  yfhH → putative DNA‑binding transcriptional regulator YfhH
RA 2,707,964 T→C N264S (AAC→AGC)  rseB ← anti‑sigma factor stabilizing protein RseB
RA 2,731,125 T→G noncoding (33/1542 nt) rrsG ← 16S ribosomal RNA
RA 2,734,706 A→G V110A (GTG→GCG)  pgeF ← polyphenol oxidase YfiH
RA 2,750,728 (T)7→6 intergenic (‑20/‑103) grpE ← / → nadK nucleotide exchange factor GrpE/NAD kinase
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,861,516 T→C G29G (GGT→GGC ygbJ → putative L‑threonate dehydrogenase
RA 2,887,647 T→C E222E (GAA→GAG cysH ← phosphoadenosine phosphosulfate reductase
RA 2,946,652 T→C N176S (AAC→AGC)  mltA ← membrane‑bound lytic murein transglycosylase A
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,958,104 (T)6→7 coding (781/2889 nt) ptrA ← protease 3
RA 2,968,268 (C)5→6 coding (167/2247 nt) ptsP ← phosphoenolpyruvate‑protein phosphotransferase PtsP
RA 2,976,386 A→T intergenic (‑1/‑213) omrB ← / → galR small regulatory RNA OmrB/DNA‑binding transcriptional dual regulator GalR
RA 2,999,755 (C)6→7 coding (137/756 nt) ygeR ← LysM domain‑containing putative peptidase lipoprotein YgeR
RA 3,016,207 A→G T50A (ACC→GCC)  ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,089,907 G→A W51* (TGG→TGA yggI → conserved protein YggI
RA 3,099,636 T→C intergenic (‑71/+46) yggM ← / ← ansB DUF1202 domain‑containing protein YggM/L‑asparaginase 2
RA 3,103,116 T→C L35P (CTC→CCC)  mutY → adenine DNA glycosylase
RA 3,175,815 A→G G64G (GGT→GGC yqiA ← esterase YqiA
RA 3,177,509 (C)6→7 coding (402/630 nt) nudF ← ADP‑sugar pyrophosphatase
RA 3,193,431 A→G T190A (ACC→GCC)  yqiK → protein YqiK
RA 3,194,843 (G)6→7 noncoding (23/145 nt) sibD ← small RNA SibD
RA 3,213,347 T→C Y101H (TAC→CAC)  rpoD → RNA polymerase, sigma 70 (sigma D) factor
RA 3,227,200 A→G K323E (AAA→GAA)  ygjI → putative transporter YgjI
RA 3,245,619 T→C R132R (CGT→CGC exuT → hexuronate transporter
RA 3,266,897 (T)5→4 coding (169/939 nt) tdcA ← DNA‑binding transcriptional activator TdcA
RA 3,308,976 A→G intergenic (‑52/+57) nlpI ← / ← pnp lipoprotein NlpI/polynucleotide phosphorylase
RA 3,309,006 (C)6→7 intergenic (‑82/+27) nlpI ← / ← pnp lipoprotein NlpI/polynucleotide phosphorylase
RA 3,350,184 (C)7→8 coding (276/654 nt) elbB ← low activity glyoxalase ElbB
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,391,146 T→C T295A (ACC→GCC)  tldD ← metalloprotease TldD
RA 3,410,526 G→A A83T (GCA→ACA)  dusB → tRNA‑dihydrouridine synthase B
RA 3,411,685 T→C G11G (GGT→GGC yhdJ → DNA adenine methyltransferase
RA 3,428,493 T→C noncoding (270/1542 nt) rrsD ← 16S ribosomal RNA
RA 3,481,747 C→G G153G (GGC→GGG yheS → ABC transporter ATP‑binding protein YheS
RA 3,548,433 T→C T546T (ACA→ACG malQ ← 4‑alpha‑glucanotransferase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,611,596 A→G D46D (GAT→GAC yhhS ← putative transporter YhhS
RA 3,635,553 T→C V238A (GTA→GCA)  yhiM → inner membrane protein with a role in acid resistance
RA 3,713,867 A→G A72A (GCA→GCG yiaC → putative acyltransferase with acyl‑CoA N‑acyltransferase domain
RA 3,729,347 T→C T9A (ACC→GCC)  xylB ← xylulokinase
RA 3,745,828 T→C V342A (GTA→GCA)  yiaN → 2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit
RA 3,751,291 C→T W193* (TGG→TAG)  yiaT ← outer membrane protein YiaT
RA 3,767,204 G→T intergenic (+25/‑17) yibA → / → yibJ putative lyase containing HEAT‑repeat/putative RHS domain‑containing protein YibJ
RA 3,774,056 T→C G592G (GGT→GGC mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,834,687 T→A I415F (ATC→TTC)  yicJ ← putative xyloside transporter YicJ
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,910,688 T→C T280A (ACC→GCC)  pstS ← phosphate ABC transporter periplasmic binding protein
RA 3,965,557 (T)8→7 coding (74/1266 nt) rhlB ← ATP‑dependent RNA helicase RhlB
RA 3,992,500 A→G T450A (ACC→GCC)  cyaA → adenylate cyclase
RA 4,054,870 T→G T137P (ACG→CCG)  glnG ← DNA‑binding transcriptional dual regulator NtrC
RA 4,074,085 T→C L116P (CTG→CCG)  yihV → 6‑deoxy‑6‑sulfofructose kinase
RA 4,075,345 A→G N226S (AAC→AGC)  yihW → putative DNA‑binding transcriptional regulator YihW
RA 4,083,951 A→G G624G (GGT→GGC fdoG ← formate dehydrogenase O subunit alpha
RA 4,198,908 A→G Q40R (CAG→CGG)  nfi → endonuclease V
RA 4,285,231 T→G intergenic (‑18/+182) yjcH ← / ← acs conserved inner membrane protein YjcH/acetyl‑CoA synthetase (AMP‑forming)
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,303,801 C→T M103I (ATG→ATA mdtN ← putative multidrug efflux pump membrane fusion protein
RA 4,304,953 A→G S549P (TCG→CCG)  yjcS ← alkyl sulfatase
RA 4,362,554 T→C noncoding (73/76 nt) pheU ← tRNA‑Phe
RA 4,394,255 C→T L64L (CTG→TTG)  nnr → NAD(P)HX epimerase/NAD(P)HX dehydratase
RA 4,410,053 (T)9→8 intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA 2'‑O‑ribose G2251 methyltransferase/conserved protein YjfI
RA 4,436,065 (T)7→8 coding (501/1944 nt) cpdB ← 2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase
RA 4,436,745 A→G intergenic (‑180/‑10) cpdB ← / → cysQ 2'3' cyclic nucleotide phosphodiesterase/3' nucleotidase/3'(2'),5'‑bisphosphate nucleotidase
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,446,396 A→G D762G (GAC→GGC)  tamB → translocation and assembly module subunit TamB
RA 4,471,253 A→G F65F (TTT→TTC pyrI ← aspartate carbamoyltransferase, PyrI subunit
RA 4,507,377 A→G pseudogene (181/255 nt) insO → KpLE2 phage‑like element; IS911B regulator fragment
RA 4,534,809 T→C intergenic (‑134/‑206) yjhZ ← / → yjhR KpLE2 phage‑like element; putative acetyltransferase YjhZ/KpLE2 phage‑like element; PLD‑like domain‑containing protein YjhR
RA 4,563,985 A→G D235D (GAT→GAC yjiL ← putative ATPase, activator of (R)‑hydroxyglutaryl‑CoA dehdratase
RA 4,590,375 A→G V302A (GTG→GCG)  btsT ← pyruvate:H(+) symporter
RA 4,606,094 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu
RA 4,606,330 (G)8→9 noncoding (72/87 nt) leuQ ← tRNA‑Leu
RA 4,624,281 A→G L170P (CTG→CCG)  ytjB ← protein Smp

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 731488–731564 733114–733095 1532–1627 11 [10] [10] 11 rhsC rhs element protein RhsC
* * ÷ NC_000913 1196292 1211423 15132 11 [9] [10] 12 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3423777–3424233 3424525–3424239 7–749 11 [9] [7] 11 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3944156–3945567 3945789–3945569 3–1634 12 [10] [10] 12 rrlC 23S ribosomal RNA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374921 =NA (NA)54 (1.620) 15/190 1.0 NA intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = NA (NA)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)33 (0.990) 16/190 0.9 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375064NA (NA)10 (0.300) 8/192 2.2 NA noncoding (33/34 nt) other REP31c
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375118NA (NA)12 (0.320) 9/216 2.3 NA noncoding (1/36 nt) other REP31d
?NC_000913 = 377333 NA (NA)intergenic (+22/+202) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 375160NA (NA)11 (0.230)
+T
9/274 3.0 NA noncoding (28/34 nt) other REP31e
?NC_000913 = 377491 NA (NA)noncoding (32/34 nt) other REP32d
* ? NC_000913 375183 =NA (NA)30 (0.720) 18/238 1.1 NA intergenic (+302/‑276) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377372 = NA (NA)noncoding (37/37 nt) other REP32a
* ? NC_000913 = 375219NA (NA)35 (0.930) 20/216 0.7 NA noncoding (1/36 nt) other REP31f
?NC_000913 = 377333 NA (NA)intergenic (+22/+202) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375248 =NA (NA)9 (0.220) 8/238 2.8 NA noncoding (15/34 nt) other REP31g
?NC_000913 377507 = NA (NA)noncoding (16/34 nt) other REP32d
* ? NC_000913 3363001 =58 (1.200)68 (1.410) 42/274 0.1 70.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)54 (1.110) 38/276 0.2 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 0 (0.000)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =27 (0.560)15 (0.330) 15/258 1.7 34.4% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 32 (0.710)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269027 (0.560)17 (0.380) 15/258 1.7 37.3% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 32 (0.710)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit