Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F28 I1 R1	164	66.7	2276140	99.1%	2255654	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,634,127	A→G	T111A (ACC→GCC)	ytjC →	putative phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,634,127	0	A	G	100.0%	151.5 / NA	41	T111A (ACC→GCC)	ytjC	putative phosphatase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (23/18); total (23/18)

CCCAGGCCTGCGGCTGTGACATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCACCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGAGTCATGGTATTGCACTGG  >  NC_000913/4633996‑4634259

cccAGGCCTGCGGCTGTGACATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGAc                                                                                                                              <  2:1071005/140‑1 (MQ=255)

     gCCTGCGGCTGTGACATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCg                                                                                                                         >  1:991633/1‑140 (MQ=255)

           ggCTGTGACATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTcc                                                                                                                   >  1:522011/1‑140 (MQ=255)

               gtgACATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGaa                                                                                                               >  1:749696/1‑140 (MQ=255)

                          tttGATTCTCGCCTGCGTGAATTAAACATGGGGGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAAt                                                                                                    >  1:593369/1‑140 (MQ=255)

                              aTTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAg                                                                                                >  1:827493/1‑140 (MQ=255)

                               ttCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAgg                                                                                               <  2:522011/140‑1 (MQ=255)

                                 ctcGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAg                                                                                             >  2:262139/1‑140 (MQ=255)

                                   cGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCt                                                                                           >  1:393136/1‑140 (MQ=255)

                                                  aaCATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCaa                                                                            <  2:130961/140‑1 (MQ=255)

                                                       gggTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCgg                                                                       <  2:827493/140‑1 (MQ=255)

                                                        ggTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGc                                                                      >  1:27794/1‑140 (MQ=255)

                                                            tgCTGGAAAAAAGACATATCTATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTg                                                                  >  2:465636/1‑140 (MQ=255)

                                                            tgCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTg                                                                  >  1:1128357/1‑140 (MQ=255)

                                                            tgCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTg                                                                  >  1:867240/1‑140 (MQ=255)

                                                                ggAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAAt                                                              <  2:867240/140‑1 (MQ=255)

                                                                     aaaGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGc                                                         <  2:570724/140‑1 (MQ=255)

                                                                           atatCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCAGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGAc                                                   >  1:793738/1‑140 (MQ=255)

                                                                                     tctGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAgg                                         <  2:393136/140‑1 (MQ=255)

                                                                                         aCCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAg                                     >  1:577980/1‑140 (MQ=255)

                                                                                            gaagaagaaGAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCg                                  >  1:209522/1‑140 (MQ=255)

                                                                                             aagaagaagAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGa                                 >  2:775820/1‑140 (MQ=255)

                                                                                             aagaagaagAGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGa                                 >  1:315305/1‑140 (MQ=255)

                                                                                              agaagaagaGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGAc                                <  2:690550/140‑1 (MQ=255)

                                                                                                  gaagaGAACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCgct                            <  1:465636/140‑1 (MQ=255)

                                                                                                      agaACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGctgctg                        <  2:577980/140‑1 (MQ=255)

                                                                                                       gaACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTgg                       <  1:775820/140‑1 (MQ=255)

                                                                                                       gaACTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTgg                       <  2:749696/140‑1 (MQ=255)

                                                                                                        aaCTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGt                      >  1:612514/1‑140 (MQ=255)

                                                                                                        aaCTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGt                      <  2:27794/140‑1 (MQ=255)

                                                                                                          cTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGa                    >  1:157511/1‑140 (MQ=255)

                                                                                                          cTGGCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGa                    >  1:287819/1‑140 (MQ=255)

                                                                                                          cTGGCGTCGGCAGCTGGTCAATAGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGa                    >  1:576399/1‑140 (MQ=255)

                                                                                                           tGGCGTCGGCAGCTGGTCAATAGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGAg                   <  2:576399/140‑1 (MQ=255)

                                                                                                            ggCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGAGt                  <  2:612514/140‑1 (MQ=255)

                                                                                                             gCGTCGGCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGAGTc                 >  1:467603/1‑140 (MQ=255)

                                                                                                                  ggCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGAGTCATGGt            >  1:817906/1‑140 (MQ=255)

                                                                                                                   gCAGCTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGAGTCATGGTa           <  2:655688/140‑1 (MQ=255)

                                                                                                                       cTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGAGTCa                <  2:39323/131‑1 (MQ=255)

                                                                                                                       cTGGTCAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGAGTCa                >  1:39323/1‑131 (MQ=255)

                                                                                                                            cAATGGCGCCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGAGTCATGGTATTGCACTgg  <  2:315305/140‑1 (MQ=255)

CCCAGGCCTGCGGCTGTGACATCATCTTTGATTCTCGCCTGCGTGAATTAAACATGGGTGTGCTGGAAAAAAGACATATCGATTCTCTGACCGAAGAAGAAGAGAACTGGCGTCGGCAGCTGGTCAATGGCACCGTTGACGGGCGTATTCCTGAAGGCGAGTCAATGCAGGAGCTCAGCGATCGCGTCAATGCGGCACTGGAATCTTGCCGGGACTTACCGCAGGGAAGCCGACCGCTGCTGGTGAGTCATGGTATTGCACTGG  >  NC_000913/4633996‑4634259

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A