breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 23,273 A→G T295A (ACG→GCG)  ileS → isoleucine‑‑tRNA ligase
RA 78,395 A→G T259A (ACG→GCG)  setA → sugar exporter SetA
RA 146,404 T→C E97E (GAA→GAG panD ← aspartate 1‑decarboxylase proenzyme
RA 163,723 G→A G540D (GGC→GAC)  hrpB → putative ATP‑dependent RNA helicase HrpB
RA 244,295 (G)5→6 intergenic (+174/‑32) lpcA → / → yafJ D‑sedoheptulose 7‑phosphate isomerase/putative amidotransferase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 265,689 C→T G133G (GGG→GGA ykfB ← CP4‑6 prophage; protein YkfB
RA 281,623 C→T G121S (GGC→AGC)  yagA ← CP4‑6 prophage; integrase core domain‑containing protein YagA
RA 308,745 C→T S196S (TCG→TCA ecpC ← putative fimbrial usher protein EcpC
RA 312,738 A→G F14L (TTC→CTC)  ykgM ← putative ribosomal protein
RA 361,790 (T)6→7 coding (71/612 nt) lacA ← galactoside O‑acetyltransferase
RA 397,808 A→G E390E (GAA→GAG sbmA → peptide antibiotic/peptide nucleic acid transporter
RA 425,133 T→C G41G (GGT→GGC queA → tRNA preQ1(34) S‑adenosylmethionine ribosyltransferase‑isomerase
RA 468,565 C→T G61G (GGC→GGT decR → DNA‑binding transcriptional activator DecR
RA 504,535 T→C E54E (GAA→GAG fsr ← fosmidomycin efflux pump
RA 522,702 T→C W763R (TGG→CGG)  ybbP → putative ABC transporter membrane subunit YbbP
RA 527,577 T→C intergenic (+36/‑4) rhsD → / → ybbC protein RhsD/PF15631 family protein YbbC
RA 532,643 C→T P65S (CCG→TCG)  allA → ureidoglycolate lyase
RA 542,589 A→G Q234Q (CAA→CAG glxK → glycerate 2‑kinase 2
RA 548,703 A→G N116S (AAC→AGC)  ylbE → DUF1116 domain‑containing protein YlbE
RA 557,620 A→G V41A (GTG→GCG)  folD ← bifunctional 5,10‑methylene‑tetrahydrofolate dehydrogenase/ 5,10‑methylene‑tetrahydrofolate cyclohydrolase
RA 560,634 T→C V313A (GTT→GCT)  sfmD → putative fimbrial usher protein SfmD
RA 566,769 C→T pseudogene (86/93 nt) renD → DLP12 prophage; protein RenD
RA 578,910 C→T intergenic (‑17/+274) borD ← / ← ybcV DLP12 prophage; prophage lipoprotein BorD/DLP12 prophage; protein YbcV
RA 616,675 A→G D840G (GAC→GGC)  entF → apo‑serine activating enzyme
RA 682,783 T→C E204G (GAA→GGA)  hscC ← chaperone protein HscC
RA 683,337 A→G I19I (ATT→ATC hscC ← chaperone protein HscC
RA 697,972 A→G V402V (GTT→GTC asnB ← asparagine synthetase B
RA 700,331 T→C intergenic (‑5/+43) umpH ← / ← nagC UMP phosphatase/DNA‑binding transcriptional dual regulator NagC
RA 724,628 C→T V118I (GTT→ATT)  kdpC ← K(+) transporting P‑type ATPase subunit KdpC
RA 737,765 A→G pseudogene (866/1062 nt) ybfL → putative transposase YbfL
RA 781,828 Δ1 bp intergenic (+176/‑257) lysQ → / → nadA tRNA‑Lys/quinolinate synthase
RA 782,737 A→G Y218C (TAC→TGC)  nadA → quinolinate synthase
RA 794,285 A→G R120R (CGT→CGC modE ← DNA‑binding transcriptional dual regulator ModE
RA 828,947 (T)5→4 coding (35/1737 nt) ybhF ← putative ABC exporter ATP binding subunit
RA 868,422 (C)8→7 coding (903/1872 nt) gsiA → glutathione ABC transporter ATP binding subunit GsiA
RA 885,736 A→G R132R (CGT→CGC ybjI ← 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 932,340 (A)8→7 intergenic (‑290/‑255) trxB ← / → lrp thioredoxin reductase/DNA‑binding transcriptional dual regulator Lrp
RA 937,408 A→G S13G (AGC→GGC)  lolA → outer membrane lipoprotein carrier protein
RA 959,171 T→C R120R (CGT→CGC aroA → 3‑phosphoshikimate 1‑carboxyvinyltransferase
RA 962,837 T→C T281T (ACT→ACC rpsA → 30S ribosomal subunit protein S1
RA 984,521 A→G *397Q (TAA→CAA)  aspC ← aspartate aminotransferase
RA 985,407 (C)6→8 coding (303/1191 nt) aspC ← aspartate aminotransferase
RA 1,010,898 C→T A312V (GCC→GTC)  uup → ATP‑binding protein with possible role in replication
RA 1,045,760 A→G V15A (GTT→GCT)  gfcE ← putative exopolysaccharide export lipoprotein GfcE
JC 1,093,358 (G)6→9 coding (483/1359 nt) dgcT → putative diguanylate cyclase DgcT
RA 1,108,336 C→T P202P (CCG→CCA opgC ← protein required for succinyl modification of osmoregulated periplasmic glucans
RA 1,128,427 G→A G197S (GGC→AGC)  murJ → putative lipid II flippase MurJ
RA 1,143,140 T→C T410A (ACT→GCT)  rne ← ribonuclease E
RA 1,220,971 G→A pseudogene (1371/2648 nt) ycgH → putative transporter component, N‑terminal fragment
RA 1,238,760 T→C L397P (CTG→CCG)  dadA → D‑amino acid dehydrogenase
RA 1,302,122 G→A R314H (CGT→CAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,436,731 T→C S55G (AGC→GGC)  ompN ← outer membrane porin N
RA 1,541,351 C→T E414K (GAA→AAA)  narZ ← nitrate reductase Z subunit alpha
RA 1,611,401 T→C T152A (ACT→GCT)  dgcF ← putative diguanylate cyclase DgcF
RA 1,660,030 T→C R654R (CGT→CGC ynfE → putative selenate reductase YnfE
RA 1,685,542 (C)6→5 coding (1047/1404 nt) fumC ← fumarase C
RA 1,710,486 A→G T95A (ACC→GCC)  rsxG → SoxR [2Fe‑2S] reducing system protein RsxG
RA 1,755,515 G→A intergenic (‑374/‑183) fumD ← / → pykF fumarase D/pyruvate kinase I
RA 1,761,634 G→A P53L (CCG→CTG)  sufD ← Fe‑S cluster scaffold complex subunit SufD
RA 1,771,496 A→G G142G (GGA→GGG ydiM → putative exporter YdiM
RA 1,815,801 T→C G645G (GGT→GGC katE → catalase II
RA 1,820,296 T→C S307G (AGT→GGT)  chbC ← N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component
RA 1,843,829 G→A intergenic (+115/+2) gdhA → / ← ynjI glutamate dehydrogenase/DUF1266 domain‑containing protein YnjI
RA 1,873,384 A→G K448K (AAA→AAG dgcJ → putative diguanylate cyclase DgcJ
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,889,776 T→C intergenic (‑30/+175) fadD ← / ← yeaY fatty acyl‑CoA synthetase/Slp family lipoprotein YeaY
RA 1,902,492 G→A A149T (GCT→ACT)  manX → mannose‑specific PTS enzyme IIAB component
RA 1,948,451 A→G V61A (GTC→GCC)  nudB ← dihydroneopterin triphosphate diphosphatase
RA 1,956,138 T→C K290K (AAA→AAG torZ ← trimethylamine N‑oxide reductase 2
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 1,999,385 T→C G32G (GGA→GGG dcyD ← D‑cysteine desulfhydrase
RA 2,022,295 C→T R143C (CGT→TGT)  fliP → flagellar biosynthesis protein FliP
RA 2,080,189 T→C T135A (ACA→GCA)  yeeA ← putative transporter YeeA
RA 2,082,980 G→A R75R (CGG→CGA sbcB → exodeoxyribonuclease I
RA 2,117,718 G→A D229D (GAC→GAT wcaK ← putative colanic acid biosynthesis pyruvyl transferase
RA 2,168,469 (G)5→6 intergenic (‑163/‑243) yegR ← / → yegS uncharacterized protein YegR/lipid kinase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,181,744 G→A A217T (GCA→ACA)  yegV → putative sugar kinase YegV
RA 2,196,727 T→C V85A (GTA→GCA)  yehF → putative protein YehF
RA 2,211,471 G→A A83T (GCC→ACC)  yehR → DUF1307 domain‑containing lipoprotein YehR
RA 2,216,679 G→A R243R (CGC→CGT yehX ← glycine betaine ABC transporter ATP binding subunit YehX
RA 2,262,984 T→C N171S (AAC→AGC)  fruB ← fructose‑specific PTS multiphosphoryl transfer protein FruB
RA 2,276,357 A→G T20A (ACC→GCC)  yejF → putative oligopeptide ABC transporter ATP binding subunit
RA 2,338,468 T→C N318S (AAC→AGC)  gyrA ← DNA gyrase subunit A
RA 2,375,217 A→G V249A (GTA→GCA)  menC ← o‑succinylbenzoate synthase
RA 2,426,387 T→C K134K (AAA→AAG hisJ ← histidine ABC transporter periplasmic binding protein
RA 2,429,397 G→A T281M (ACG→ATG)  purF ← amidophosphoribosyltransferase
RA 2,432,226 G→A S239S (AGC→AGT folC ← bifunctional folylpolyglutamate synthetase/dihydrofolate synthetase
RA 2,446,579 T→C T299A (ACC→GCC)  aroC ← chorismate synthase
RA 2,455,873 G→A intergenic (‑227/+454) yfcV ← / ← sixA putative fimbrial protein YfcV/phosphohistidine phosphatase
RA 2,456,234 A→G intergenic (‑588/+93) yfcV ← / ← sixA putative fimbrial protein YfcV/phosphohistidine phosphatase
RA 2,471,866 A→G pseudogene (197/519 nt) yfdL ← CPS‑53 (KpLE1) prophage; putative uncharacterized protein YfdL
RA 2,527,823 T→C V293A (GTG→GCG)  yfeH → putative solute:Na(+) symporter
RA 2,602,017 (C)7→8 coding (200/2019 nt) hyfB → hydrogenase 4 component B
RA 2,656,054 T→C N102S (AAC→AGC)  pepB ← aminopeptidase B
RA 2,693,457 G→A A696V (GCG→GTG)  purL ← phosphoribosylformylglycinamide synthetase
RA 2,717,568 C→T G321S (GGC→AGC)  yfiF ← putative methyltransferase
RA 2,735,938 G→A A25V (GCC→GTC)  rluD ← 23S rRNA pseudouridine(1911/1915/1917) synthase
RA 2,749,698 T→C Y309H (TAT→CAT)  yfjD → putative inner membrane protein
RA 2,767,507 (C)7→9 intergenic (+152/‑203) rnlB → / → yfjP CP4‑57 prophage; antitoxin RnlB/CP4‑57 prophage; putative GTP‑binding protein YfjP
RA 2,773,416 (T)8→7 coding (99/1704 nt) yfjW → CP4‑57 prophage; uncharacterized protein YfjW
RA 2,776,702 T→C I106T (ATA→ACA)  yfjY → CP4‑57 prophage; RadC‑like JAB domain‑containing protein YfjY
RA 2,793,716 G→A G328R (GGG→AGG)  gabT → 4‑aminobutyrate aminotransferase GabT
RA 2,811,134 A→G H122R (CAC→CGC)  mprA → DNA‑binding transcriptional repressor MprA
RA 2,828,224 G→A G163E (GGG→GAG)  srlD → sorbitol‑6‑phosphate 2‑dehydrogenase
RA 2,852,333 T→C G66G (GGT→GGC hypD → Fe‑(CN)2CO cofactor assembly scaffold protein HypD
MC JC 2,857,096 Δ2,538 bp coding (4‑2541/2562 nt) mutS → DNA mismatch repair protein MutS
RA 2,883,098 (C)6→7 coding (1041/1509 nt) casA ← type I‑E CRISPR system Cascade subunit CasA
RA 2,884,500 G→A intergenic (‑362/+53) casA ← / ← cas3 type I‑E CRISPR system Cascade subunit CasA/CRISPR‑associated endonuclease/helicase Cas3
RA 2,937,868 T→C F134S (TTC→TCC)  fucK → L‑fuculokinase
RA 2,942,901 (T)8→7 intergenic (+1/+17) gcvB → / ← ygdI small regulatory RNA GcvB/DUF903 domain‑containing lipoprotein YgdI
RA 2,943,712 T→C W126R (TGG→CGG)  csdA → cysteine sulfinate desulfinase
RA 2,949,993 (T)5→6 coding (752/1332 nt) argA → N‑acetylglutamate synthase
RA 2,950,940 T→C T508A (ACG→GCG)  recD ← exodeoxyribonuclease V subunit RecD
RA 3,014,973 (C)6→7 coding (85/771 nt) yqeC ← uncharacterized protein YqeC
RA 3,068,157 T→C intergenic (‑77/+16) yggE ← / ← argO uncharacterized protein YggE/L‑arginine exporter
RA 3,123,682 T→C I42V (ATC→GTC)  glcB ← malate synthase G
RA 3,142,974 T→C T6A (ACT→GCT)  hybC ← hydrogenase 2 large subunit
RA 3,148,684 A→G N263S (AAC→AGC)  gpr → L‑glyceraldehyde 3‑phosphate reductase
RA 3,155,841 T→C F163L (TTC→CTC)  yqhD → NADPH‑dependent aldehyde reductase YqhD
RA 3,177,829 (A)7→6 coding (82/630 nt) nudF ← ADP‑sugar pyrophosphatase
RA 3,196,990 T→C E884E (GAA→GAG glnE ← fused glutamine synthetase deadenylase/glutamine synthetase adenylyltransferase
RA 3,201,912 G→A G8S (GGT→AGT)  cca → fused tRNA nucleotidyltransferase/2',3'‑cyclic phosphodiesterase/2' nucleotidase and phosphatase
RA 3,208,223 T→C I67T (ATA→ACA)  ttdT → L‑tartrate:succinate antiporter
RA 3,225,837 A→G H39R (CAC→CGC)  ebgC → DUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta
RA 3,233,478 T→C L605P (CTG→CCG)  fadH → 2,4‑dienoyl‑CoA reductase
RA 3,233,669 G→A A669T (GCG→ACG)  fadH → 2,4‑dienoyl‑CoA reductase
RA 3,237,401 G→A V31I (GTA→ATA)  ygjR → putative oxidoreductase YgjR
RA 3,281,585 T→C pseudogene (464/504 nt) agaA → putative truncated N‑acetylgalactosamine‑6‑phosphate deacetylase
RA 3,299,839 T→C E26G (GAA→GGA)  yhbP ← putative FMN binding protein YhbP
RA 3,320,534 C→T L360L (CTG→CTA yhbX ← putative hydrolase, inner membrane
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,388,726 A→G V134A (GTT→GCT)  aaeA ← aromatic carboxylic acid efflux pump membrane fusion protein
RA 3,404,262 T→C V260A (GTC→GCC)  yhdH → acrylyl‑CoA reductase
RA 3,446,311 T→C S79G (AGC→GGC)  rpsH ← 30S ribosomal subunit protein S8
RA 3,546,408 T→C intergenic (+209/‑151) nfuA → / → gntT iron‑sulfur cluster carrier protein NfuA/high‑affinity gluconate transporter
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,574,808 T→C E24G (GAG→GGG)  asd ← aspartate‑semialdehyde dehydrogenase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,833,177 T→C D454G (GAC→GGC)  yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,860,630 (C)5→4 coding (348/894 nt) yidL → putative DNA‑binding transcriptional regulator YidL
RA 3,931,183 (G)7→8 intergenic (‑90/‑133) ravA ← / → kup regulatory ATPase RavA/K(+):H(+) symporter Kup
RA 3,961,617 T→C V314A (GTA→GCA)  rep → ATP‑dependent DNA helicase Rep
RA 4,011,323 C→T T83M (ACG→ATG)  bioP → biotin transporter
RA 4,054,887 A→G V131A (GTT→GCT)  glnG ← DNA‑binding transcriptional dual regulator NtrC
RA 4,231,149 (A)6→5 coding (796/873 nt) rluF → 23S rRNA pseudouridine(2604) and tRNA(Tyr) pseudouridine(35) synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,307,957 A→G G174G (GGT→GGC alsE ← D‑allulose‑6‑phosphate 3‑epimerase
RA 4,330,149 A→G T264A (ACT→GCT)  yjcZ → uncharacterized protein YjcZ
RA 4,364,285 G→C T253S (ACC→AGC)  dsbD ← protein disulfide oxidoreductase ‑ DsbDreduced
RA 4,372,570 A→G T516A (ACC→GCC)  groL → chaperonin GroEL
RA 4,399,552 A→G I101V (ATC→GTC)  miaA → tRNA dimethylallyltransferase
RA 4,445,549 A→G T480A (ACC→GCC)  tamB → translocation and assembly module subunit TamB
RA 4,448,745 T→A D18E (GAT→GAA chpB → endoribonuclease toxin ChpB
RA 4,452,023 A→G K322K (AAA→AAG ytfR → galactofuranose ABC transporter putative ATP binding subunit
RA 4,454,722 A→G R296R (CGT→CGC fbp ← fructose‑1,6‑bisphosphatase 1
RA 4,512,124 (C)5→6 coding (291/957 nt) fecD ← ferric citrate ABC transporter membrane subunit FecD
RA 4,589,904 A→G V459A (GTC→GCC)  btsT ← pyruvate:H(+) symporter
RA 4,606,094 (G)8→9 noncoding (72/87 nt) leuV ← tRNA‑Leu
RA 4,606,330 (G)8→9 noncoding (72/87 nt) leuQ ← tRNA‑Leu
RA 4,630,068 C→T R152H (CGT→CAT)  ettA ← energy‑dependent translational throttle protein EttA
RA 4,634,127 A→G T111A (ACC→GCC)  ytjC → putative phosphatase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1633305–1633620 1634911–1634644 1025–1607 19 [15] [16] 20 [ydfK]–[ydfN] [ydfK],pinQ,tfaQ,[ydfN]
* * ÷ NC_000913 3423768–3424234 3424528–3424238 5–761 18 [16] [16] 20 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374921 =NA (NA)54 (1.180) 19/190 1.2 NA intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = NA (NA)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)46 (1.000) 17/190 1.4 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375023 =NA (NA)13 (0.280) 11/192 2.5 NA intergenic (+142/‑436) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377530 = NA (NA)intergenic (+219/+5) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375102 =NA (NA)32 (0.610) 11/216 2.9 NA noncoding (17/36 nt) other REP31d
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 375183 =NA (NA)23 (0.400) 14/238 2.6 NA intergenic (+302/‑276) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377372 = NA (NA)noncoding (37/37 nt) other REP32a
* ? NC_000913 375203 =NA (NA)31 (0.590) 14/216 2.3 NA noncoding (17/36 nt) other REP31f
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 1207790 =11 (0.160)66 (1.120) 41/244 0.3 86.4% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 11 (0.190)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780511 (0.160)45 (0.760) 34/244 0.5 81.3% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 11 (0.190)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)67 (1.030) 43/268 0.3 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =121 (1.810)119 (1.800) 61/274 0.1 66.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)59 (0.880) 40/276 0.5 95.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.040)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =41 (0.610)17 (0.270) 16/258 2.5 27.8% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 50 (0.800)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit