Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F28 I1 R1	126	51.4	1736588	99.0%	1719222	138.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,368,107	A→G	Y94C (TAC→TGC)	yhcG →	DUF1016 domain‑containing protein YhcG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,368,107	0	A	G	100.0%	123.7 / NA	36	Y94C (TAC→TGC)	yhcG	DUF1016 domain‑containing protein YhcG
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/20); total (16/20)

ATTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCC  >  NC_000913/3367971‑3368240

atggTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCct                                                                                                                                    <  2:531522/137‑1 (MQ=255)

aTTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCct                                                                                                                                    <  1:655048/140‑1 (MQ=255)

        aTTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTc                                                                                                                            <  1:5755/140‑1 (MQ=255)

         tttGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCa                                                                                                                           >  1:335080/1‑140 (MQ=255)

                      gCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAAtt                                                                                                              <  2:243914/140‑1 (MQ=255)

                         aGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCAc                                                                                                           <  1:676589/140‑1 (MQ=255)

                            ccAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAg                                                                                                        >  1:155615/1‑140 (MQ=255)

                                ggCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACaa                                                                                                    >  1:423913/1‑140 (MQ=255)

                                     cGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCt                                                                                               >  2:170685/1‑140 (MQ=255)

                                       tATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTgg                                                                                             <  2:423913/140‑1 (MQ=255)

                                        aTGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGc                                                                                            <  2:155615/140‑1 (MQ=255)

                                          ggTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGa                                                                                          >  2:744062/1‑140 (MQ=255)

                                            tGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAAt                                                                                        <  2:33583/140‑1 (MQ=255)

                                               gcAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGa                                                                                                       >  2:813674/1‑122 (MQ=255)

                                               gcAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGa                                                                                                       <  1:813674/122‑1 (MQ=255)

                                               gcAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTaa                                                                                     >  2:159480/1‑140 (MQ=255)

                                               gcAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTaa                                                                                     <  1:499414/140‑1 (MQ=255)

                                                 aGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAaca                                                                                   >  2:480776/1‑140 (MQ=255)

                                                           gCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGaa                                                                         <  1:170685/140‑1 (MQ=255)

                                                            cGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAAt                                                                        <  1:159480/140‑1 (MQ=255)

                                                                         aTTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCg                                                           >  2:123688/1‑140 (MQ=255)

                                                                            tATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCt                                                        >  1:630904/1‑140 (MQ=255)

                                                                            tATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCt                                                        >  1:242121/1‑140 (MQ=255)

                                                                                         taAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCAt                                           >  2:351257/1‑140 (MQ=255)

                                                                                                gggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaaa                                    >  1:224451/1‑140 (MQ=255)

                                                                                                gggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCaaa                                    >  1:711154/1‑140 (MQ=255)

                                                                                                 ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAg                                   >  2:574653/1‑140 (MQ=255)

                                                                                                 ggTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAg                                   >  2:824301/1‑140 (MQ=255)

                                                                                                  gTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGa                                  <  1:480776/140‑1 (MQ=255)

                                                                                                   ttCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGAt                                 <  2:711154/140‑1 (MQ=255)

                                                                                                        tGCAAAAAACTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGc                            <  2:224451/140‑1 (MQ=255)

                                                                                                              aaaCTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGc                      <  1:351257/140‑1 (MQ=255)

                                                                                                               aaCTTACGCCAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCt                     <  1:123688/140‑1 (MQ=255)

                                                                                                                        cAAATGAGGCTTTTTTGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCaa            <  2:88258/140‑1 (MQ=255)

                                                                                                                                  ttttttGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcccc  <  2:242121/140‑1 (MQ=255)

                                                                                                                                  ttttttGCCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTcccc  <  2:630904/140‑1 (MQ=255)

ATTGTCGAATTTGAACAAGGTGGCGAGGCCAGGGCTGCGTATGGTGCGCAGCTAATCAAGCGACTATCAAAGGATTTATGTCTAAGGTATAAGCGTGGGTTCTCTGCAAAAAACTTACGCCAAATGAGGCTTTTTTACCTCTTTTTTCAACATGTTGAAATTCACCAGACAATGTCTGGCGAATTAACACCATTGGGAATTCCGCAGACACCGTCTGCAGAATTCCCATCGGCAAAGATTTGGCAGACGCTGTCTGCCAAATCTTTCCCC  >  NC_000913/3367971‑3368240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A