Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F28 I1 R1	176	31.5	1082462	98.8%	1069472	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,373,213	A→G	Y122H (TAT→CAT)	nanA ←	N‑acetylneuraminate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,373,213	0	A	G	100.0%	122.6 / NA	34	Y122H (TAT→CAT)	nanA	N‑acetylneuraminate lyase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (16/18); total (16/18)

GTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGACGTGGGCGA  >  NC_000913/3373081‑3373347

gTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCg                                                                                                                                 <  2:508068/140‑1 (MQ=255)

  ttCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCa                                                                                                                               <  1:248340/140‑1 (MQ=255)

      gcgcACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCa                                                                                                                               <  2:52839/136‑1 (MQ=255)

      gcgcACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCa                                                                                                                               >  1:52839/1‑136 (MQ=255)

               cGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGa                                                                                                                                    >  1:153245/1‑122 (MQ=255)

               cGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGa                                                                                                                                    <  2:153245/122‑1 (MQ=255)

                 ccAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCt                                                                                                                <  2:293772/140‑1 (MQ=255)

                      cAATGTAACAAGTGTGTTGATCTGATCCAGGTTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAg                                                                                                           <  1:116187/140‑1 (MQ=255)

                      cAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAg                                                                                                           <  1:127112/140‑1 (MQ=255)

                      cAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAg                                                                                                           <  1:460145/140‑1 (MQ=255)

                                   gtgtTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACgg                                                                                              >  1:104392/1‑140 (MQ=255)

                                    tgtTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACggg                                                                                             >  2:76954/1‑139 (MQ=255)

                                    tgtTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCACGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGc                                                                                             >  1:85084/1‑140 (MQ=255)

                                         atctgatcCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGAc                                                                                        >  1:175568/1‑140 (MQ=255)

                                           ctgatcCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGAcgg                                                                                      >  1:490891/1‑140 (MQ=255)

                                                   gggTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACg                                                                              >  2:56337/1‑140 (MQ=255)

                                                               ccccACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCa                                                                  <  2:104392/140‑1 (MQ=255)

                                                                    cTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACg                                                             >  2:250861/1‑140 (MQ=255)

                                                                         ggCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAg                                                        >  2:502390/1‑140 (MQ=255)

                                                                          gCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGc                                                       >  2:312633/1‑140 (MQ=255)

                                                                               aaTGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATg                                                  <  2:175568/140‑1 (MQ=255)

                                                                                        caccacCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAgtt                                         >  2:251434/1‑140 (MQ=255)

                                                                                           caccaTCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCg                                               <  1:423751/131‑1 (MQ=255)

                                                                                           caccaTCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCg                                               >  2:423751/1‑131 (MQ=255)

                                                                                             ccaTCGGCAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTgg                                    >  1:224957/1‑140 (MQ=255)

                                                                                                    cAAACCATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTcgg                             <  1:439956/140‑1 (MQ=255)

                                                                                                         cATCCGCCGAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTg                        <  2:224957/140‑1 (MQ=255)

                                                                                                             cgccgAATCAATAATTGCCCGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGa                    <  1:227687/140‑1 (MQ=255)

                                                                                                                              cccGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGACg         <  2:206810/134‑1 (MQ=255)

                                                                                                                              cccGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGACg         >  1:206810/1‑134 (MQ=255)

                                                                                                                              cccGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGACGTGGGCg   >  1:66470/1‑140 (MQ=255)

                                                                                                                               ccGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGACGTGGGCGa  <  1:251434/140‑1 (MQ=255)

                                                                                                                               ccGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGACGTGGGCGa  <  1:502390/140‑1 (MQ=255)

                                                                                                                               ccGATGGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGACGTGGGCGa  <  2:66470/140‑1 (MQ=255)

GTTTCAGCGCACCTACGCCAGGCAATGTAACAAGTGTGTTGATCTGATCCAGGGTCAGTTTTACCCCACTCAGGGCTGGAATGTTGTACACCACCATCGGCAAACCATCCGCCGAATCAATAATTGCCCGATAGTGATCGCAGTGTTCTTCAAAGCTGAAAGGATAGTAGAACGGCGTGACGGCGGAGACGGCATCGAAGCCATAACGTTTAGCCGATGCCGCAAGTTGTTGGCTTTCGGCGGTGCTGACGCAACCGACGTGGGCGA  >  NC_000913/3373081‑3373347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A