Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A31 F30 I1 R1	28	44.1	1499574	99.3%	1489076	139.8

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,951,529	G→T	G311G (GGC→GGA)	recD ←	exodeoxyribonuclease V subunit RecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,951,529	0	G	T	100.0%	145.3 / NA	42	G311G (GGC→GGA)	recD	exodeoxyribonuclease V subunit RecD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (15/27); total (15/27)

TGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCATCTACCAC  >  NC_000913/2951395‑2951663

tGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAg                                                                                                                                   <  1:544355/140‑1 (MQ=255)

    gcaggcagAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGcaca                                                                                                                               <  1:138759/140‑1 (MQ=255)

       ggcagAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGcc                                                                                                                            <  2:229830/140‑1 (MQ=255)

       ggcagAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGcc                                                                                                                            >  1:568447/1‑140 (MQ=255)

              aCTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCt                                                                                                                     <  2:296160/140‑1 (MQ=255)

               cTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTc                                                                                                                    >  2:171885/1‑140 (MQ=255)

                 gTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCaa                                                                                                                  >  2:335303/1‑140 (MQ=255)

                  tCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAAc                                                                                                                 >  1:550014/1‑140 (MQ=255)

                  tCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAAc                                                                                                                 >  1:352979/1‑140 (MQ=255)

                   cgcgCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAAcc                                                                                                                >  2:224006/1‑140 (MQ=255)

                      gcAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAg                                                                                                             <  2:73041/140‑1 (MQ=255)

                       cAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAgg                                                                                                            >  2:725188/1‑140 (MQ=255)

                        aaaGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCGGCACAGCCCCAGCCTCAACCGAGGc                                                                                                           <  2:490115/140‑1 (MQ=255)

                           gATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAg                                                                                                        <  1:382327/140‑1 (MQ=255)

                           gATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAg                                                                                                        <  1:214182/140‑1 (MQ=255)

                              gCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTg                                                                                                     >  2:260936/1‑140 (MQ=255)

                                    tCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACg                                                                                               <  2:593598/140‑1 (MQ=255)

                                    tCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACg                                                                                               <  1:98776/140‑1 (MQ=255)

                                      tgtgCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGAt                                                                                             >  1:39407/1‑140 (MQ=255)

                                         gCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCATCCTCAACCGAGGCCAGTTGATCACGATCGc                                                                                          <  2:550014/140‑1 (MQ=255)

                                            ggTTCCGGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCga                                                                                       <  1:134946/140‑1 (MQ=255)

                                                ccTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGaaa                                                                                   <  1:479684/140‑1 (MQ=255)

                                                  tGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGa                                                                                 >  1:609405/1‑140 (MQ=255)

                                                      ggAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCAc                                                                             <  1:167845/140‑1 (MQ=255)

                                                        aaCGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTc                                                                           >  2:580838/1‑140 (MQ=255)

                                                                  cccGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATc                                                                 <  2:568447/140‑1 (MQ=255)

                                                                       cAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCa                                                            <  1:279245/140‑1 (MQ=255)

                                                                       cAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCa                                                            <  1:493579/140‑1 (MQ=255)

                                                                             gcTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGt                                                      >  2:254248/1‑140 (MQ=255)

                                                                                        tGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGtctc                                           <  1:224006/140‑1 (MQ=255)

                                                                                         ggCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCg                                          <  1:634410/140‑1 (MQ=255)

                                                                                           cACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGAc                                        <  1:533832/140‑1 (MQ=255)

                                                                                               cTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGAcatca                                    <  1:580838/140‑1 (MQ=255)

                                                                                                    cggTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGc                               >  1:470541/1‑140 (MQ=255)

                                                                                                       tAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCGAGTTGATCACGGTCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAga                            <  2:736151/140‑1 (MQ=255)

                                                                                                        aaaGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAgat                           <  1:514218/140‑1 (MQ=255)

                                                                                                            cccGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAgatcgat                       >  2:520924/1‑140 (MQ=255)

                                                                                                              cgcgTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCa                     >  2:5470/1‑140 (MQ=255)

                                                                                                                cgTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCAtt                   <  2:352979/140‑1 (MQ=255)

                                                                                                                   tGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGAc                <  2:545339/140‑1 (MQ=255)

                                                                                                                       aTAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCtt            <  1:582305/140‑1 (MQ=255)

                                                                                                                          aGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCAt         <  1:565491/140‑1 (MQ=255)

                                                                                                                          aGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCAt         <  1:260936/140‑1 (MQ=255)

                                                                                                                                 atatCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCATCTaccac  <  2:343371/140‑1 (MQ=255)

TGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCATCTACCAC  >  NC_000913/2951395‑2951663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A