Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A14 F30 I1 R1 20 70.7 2392400 99.2% 2373260 138.6

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,659,493 G→T intergenic (‑26/+70) hscB ← / ← iscA co‑chaperone for [Fe‑S] cluster biosynthesis/iron‑sulfur cluster insertion protein IscA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,659,4930GT100.0% 183.5 / NA 52intergenic (‑26/+70)hscB/iscAco‑chaperone for [Fe‑S] cluster biosynthesis/iron‑sulfur cluster insertion protein IscA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/24);  total (28/24)

TCAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCT  >  NC_000913/2659367‑2659629
                                                                                                                              |                                                                                                                                        
tCAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGaa                                                                                                                             <  2:366613/140‑1 (MQ=255)
tCAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGaa                                                                                                                             <  1:926858/140‑1 (MQ=255)
 cAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAAt                                                                                                                            <  2:62695/140‑1 (MQ=255)
     atgatACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAgg                                                                                                                        <  2:521092/140‑1 (MQ=255)
       gatACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCCGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTa                                                                                                                      >  2:153794/1‑140 (MQ=255)
         tACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTaaa                                                                                                                    >  1:1101670/1‑140 (MQ=255)
              aCGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGaa                                                                                                                             >  1:782394/1‑126 (MQ=255)
              aCGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGaa                                                                                                                             <  2:782394/126‑1 (MQ=255)
              aCGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAaaacaaa                                                                                                               >  2:574875/1‑140 (MQ=255)
              aCGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAaaacaaa                                                                                                               >  2:182636/1‑140 (MQ=255)
                  tGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAAcccc                                                                                                           >  1:431949/1‑140 (MQ=255)
                   gTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCa                                                                                                          >  1:18153/1‑140 (MQ=255)
                    tAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCAc                                                                                                         >  1:129172/1‑140 (MQ=255)
                       aTCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCAcgcg                                                                                                      <  2:431949/140‑1 (MQ=255)
                         ccTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCa                                                                                                    <  1:303229/140‑1 (MQ=255)
                         ccTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCa                                                                                                    >  1:7999/1‑140 (MQ=255)
                           tGAAAACGCACGCTCAGCGCCTGGGAATCGAGGTGATAGCGGGCAGACAAGCCAAAGAGGGTGCAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGGAAAACAAACCCCACGCGCAgg                                                                                                  >  2:941193/1‑140 (MQ=255)
                              aaaCGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCCGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGa                                                                                               <  1:153794/140‑1 (MQ=255)
                                 cGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCa                                                                                            >  2:388634/1‑140 (MQ=255)
                                     ggCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGt                                                                                        <  2:716417/140‑1 (MQ=255)
                                     ggCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGt                                                                                        <  2:18153/140‑1 (MQ=255)
                                     ggCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGt                                                                                        <  1:388634/140‑1 (MQ=255)
                                         cAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTgggg                                                                                    <  2:1101670/140‑1 (MQ=255)
                                           gcgcCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGtt                                                                                  <  2:1030715/140‑1 (MQ=255)
                                            cgcCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTa                                                                                 >  2:1058313/1‑140 (MQ=255)
                                                      tCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATgcg                                                                       >  1:322413/1‑140 (MQ=255)
                                                                    ggcaggcaAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGaa                                                         >  1:14216/1‑140 (MQ=255)
                                                                              ccAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTcgccg                                               <  1:1058313/140‑1 (MQ=255)
                                                                                  agagGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAAc                                           <  2:7999/140‑1 (MQ=255)
                                                                                        tGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTcgccg                                               <  1:669975/130‑1 (MQ=255)
                                                                                        tGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTcgccg                                               >  2:669975/1‑130 (MQ=255)
                                                                                        tGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGAGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACt                                     >  2:622428/1‑140 (MQ=255)
                                                                                                tCCATAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTg                             >  1:691034/1‑140 (MQ=255)
                                                                                                    tAACATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGt                         >  1:364574/1‑140 (MQ=255)
                                                                                                     aaCATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGtt                        >  1:400982/1‑140 (MQ=255)
                                                                                                     aaCATTCTCAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGtt                        <  1:805317/140‑1 (MQ=255)
                                                                                                          tctcAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGt                   >  1:1023767/1‑140 (MQ=255)
                                                                                                          tctcAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGt                   >  1:804363/1‑140 (MQ=255)
                                                                                                            tcAGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTg                 >  1:552606/1‑140 (MQ=255)
                                                                                                              aGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGt               >  2:190312/1‑140 (MQ=255)
                                                                                                              aGGGCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGt               <  2:322413/140‑1 (MQ=255)
                                                                                                               gggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGtt                        <  2:954056/130‑1 (MQ=255)
                                                                                                               gggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGtt                        >  1:954056/1‑130 (MQ=255)
                                                                                                               gggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTg              <  1:182636/140‑1 (MQ=255)
                                                                                                                ggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCtt                               >  2:1056246/1‑122 (MQ=255)
                                                                                                                ggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCtt                               <  1:1056246/122‑1 (MQ=255)
                                                                                                                ggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCAGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCggg                    <  2:587386/133‑1 (MQ=255)
                                                                                                                ggCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCAGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCggg                    >  1:587386/1‑133 (MQ=255)
                                                                                                                 gCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCggg                    >  1:839385/1‑132 (MQ=255)
                                                                                                                 gCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCggg                    <  2:839385/132‑1 (MQ=255)
                                                                                                                 gCTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGaa            >  2:108944/1‑140 (MQ=255)
                                                                                                                  cTGCGATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAAt           >  1:827332/1‑140 (MQ=255)
                                                                                                                      gATCCGCCTCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTaa       <  2:1158908/140‑1 (MQ=255)
                                                                                                                          cgcctcGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAAccc   <  2:387198/140‑1 (MQ=255)
                                                                                                                          cgcctcGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAAccc   >  2:1132216/1‑140 (MQ=255)
                                                                                                                          cgcctcGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAAccc   >  1:387198/1‑140 (MQ=255)
                                                                                                                           gcctcGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCt  <  1:108944/140‑1 (MQ=255)
                                                                                                                              |                                                                                                                                        
TCAGGATGATACTGACGTTGTAGATCCTGAAAACGCAGGCTCAGCGCCTGGGTATCGAGTTGATAGCGGGCAGGCAAGCCAAAGAGGGTGAAGTAATCCATAACATTCTCAGGGCTGCGATCCGCCGCGATAACGGCGAATCAGGTAAAACAAACCCCACGCGCAGGCGACCACGGTGGGGTTATCGGTATGCGCATCAAACGTGGAAGCTTTCGCCGCAACCACACTCATCTTTGACGTTCGGGTTGGTGAATTTAAACCCT  >  NC_000913/2659367‑2659629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A