Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A20 F30 I1 R1	25	54.5	1844758	99.1%	1828155	139.4

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,541,787	C→A	L461M (CTG→ATG)	feoB →	Fe(2(+)) transporter FeoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,541,787	0	C	A	96.2%	176.2 / ‑0.6	53	coding (1381/2322 nt)	feoB	Fe(2(+)) transporter FeoB
Reads supporting (aligned to +/- strand): ref base C (0/0); major base A (20/31); minor base . (1/1); total (21/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

GCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGC  >  NC_000913/3541652‑3541922

gCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATggg                                                                                                                                     <  2:43989/140‑1 (MQ=255)

 cACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGt                                                                                                                                    <  1:333694/140‑1 (MQ=255)

   ccGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTAt                                                                                                                                  >  1:140711/1‑140 (MQ=255)

     gcgTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATtg                                                                                                                                >  1:208760/1‑140 (MQ=255)

     gcgTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATtg                                                                                                                                >  1:224157/1‑140 (MQ=255)

     gcgTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATtg                                                                                                                                >  1:641635/1‑140 (MQ=255)

               cTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGt                                                                                                                      <  1:580931/140‑1 (MQ=255)

                         tcatGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGc                                                                                                            >  2:430719/1‑140 (MQ=255)

                         tcatGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGc                                                                                                            >  2:611962/1‑140 (MQ=255)

                          catGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGcc                                                                                                           <  1:430719/140‑1 (MQ=255)

                                ggCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGc                                                                                                     >  1:188290/1‑140 (MQ=255)

                                  cACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTc                                                                                                   <  1:441870/140‑1 (MQ=255)

                                  cACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTc                                                                                                   <  2:838429/140‑1 (MQ=255)

                                            gTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACca                                                                                         >  2:351834/1‑140 (MQ=255)

                                               cTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGCGCTGACTGGCCTGATGCTCAAGTACACcatca                                                                                      >  2:773846/1‑140 (MQ=255)

                                               cTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATA‑‑‑TGAGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATgc                                                                                   >  1:476343/1‑140 (MQ=255)

                                                tGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACcatcat                                                                                     <  2:322032/140‑1 (MQ=255)

                                                 gcggcgCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATg                                                                                    >  2:483104/1‑140 (MQ=255)

                                                  cggcgCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATgc                                                                                   >  2:53092/1‑140 (MQ=255)

                                                   ggcgCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATgcg                                                                                  <  1:236955/140‑1 (MQ=255)

                                                   ggcgCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATgcg                                                                                  <  1:626153/140‑1 (MQ=255)

                                                       cgcgTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGt                                                                              >  1:379326/1‑140 (MQ=255)

                                                              ggCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGa                                                                       <  1:552675/140‑1 (MQ=255)

                                                              ggCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGa                                                                       <  2:224157/140‑1 (MQ=255)

                                                                    cTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGt                                                                 >  2:546817/1‑140 (MQ=255)

                                                                    cTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGt                                                                 <  2:273472/140‑1 (MQ=255)

                                                                     ttCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGtt                                                                <  2:413071/140‑1 (MQ=255)

                                                                      tCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGttt                                                               <  1:483104/140‑1 (MQ=255)

                                                                      tCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATA‑‑‑TGAGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTc                                                            <  2:476343/140‑1 (MQ=255)

                                                                         cAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTc                                                            >  2:614895/1‑140 (MQ=255)

                                                                                cgcgGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGt                                                     <  1:546817/140‑1 (MQ=255)

                                                                                 gcgGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGtt                                                    >  1:814975/1‑140 (MQ=255)

                                                                                 gcgGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGtt                                                    >  2:816483/1‑140 (MQ=255)

                                                                                    gCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGcc                                                 <  1:291728/140‑1 (MQ=255)

                                                                                      tGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCgg                                               >  2:743717/1‑140 (MQ=255)

                                                                                      tGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCgg                                               >  2:917456/1‑140 (MQ=255)

                                                                                      tGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCgg                                               >  2:356318/1‑140 (MQ=255)

                                                                                       gCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGt                                              <  1:446545/140‑1 (MQ=255)

                                                                                       gCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGt                                              <  2:172210/140‑1 (MQ=255)

                                                                                          ttcttcGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATTATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTa                                           <  2:707523/140‑1 (MQ=255)

                                                                                          ttcttcGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTa                                           <  2:802136/140‑1 (MQ=255)

                                                                                           tcttcGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTAt                                          <  2:171053/140‑1 (MQ=255)

                                                                                            cttcGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATc                                         <  2:641635/140‑1 (MQ=255)

                                                                                                  gCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTAc                                   <  1:53092/140‑1 (MQ=255)

                                                                                                          gTGCGCTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTACCACACGtt                           <  1:816483/140‑1 (MQ=255)

                                                                                                              gcTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTACCACACGTTaaaa                       <  2:360988/140‑1 (MQ=255)

                                                                                                               cTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAg                      <  2:379326/140‑1 (MQ=255)

                                                                                                               cTGGCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAg                      <  1:752945/140‑1 (MQ=255)

                                                                                                                  gCGGTCTTCTCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCt                   >  2:630881/1‑140 (MQ=255)

                                                                                                                        ttctCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTAt             <  1:917456/140‑1 (MQ=255)

                                                                                                                        ttctCGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTAt             <  1:351834/140‑1 (MQ=255)

                                                                                                                          ctcGCTGTATATGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATcc           <  1:614895/140‑1 (MQ=255)

                                                                                                                                   tatGATGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGc  <  2:188290/140‑1 (MQ=255)

GCACCGCGTGAACGTCTGATGACCATCATGATGGCACCGTTTATGTCCTGCGGCGCGCGTCTGGCTATCTTCGCAGTATTCGCGGCTGCCTTCTTCGGGCAGAACGGTGCGCTGGCGGTCTTCTCGCTGTATATGCTGGGTATTGTGATGGCGGTGCTGACTGGCCTGATGCTCAAGTACACCATCATGCGCGGTGAAGCGACGCCGTTTGTCATGGAGTTGCCGGTCTATCATGTACCACACGTTAAAAGCCTGATTATCCAGACCTGGC  >  NC_000913/3541652‑3541922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A