Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A36 F30 I1 R1	19	98.9	3364616	99.3%	3341063	138.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,731,280	T→C	intergenic (‑123/+320)	rrsG ← / ← clpB	16S ribosomal RNA/ClpB chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,731,280	0	T	C	100.0%	67.4 / NA	21	intergenic (‑123/+320)	rrsG/clpB	16S ribosomal RNA/ClpB chaperone
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (15/6); total (15/6)

GCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACC  >  NC_000913/2731238‑2731416

gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         <  1:1089914/140‑1 (MQ=18)

gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         >  1:141881/1‑140 (MQ=18)

gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         <  2:657808/140‑1 (MQ=18)

gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         >  1:1641058/1‑140 (MQ=18)

gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         >  1:808633/1‑140 (MQ=18)

gCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                         >  1:844686/1‑140 (MQ=18)

      ttAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                                   >  2:69630/1‑140 (MQ=255)

      ttAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                                   <  2:679502/140‑1 (MQ=38)

      ttAAGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                                   >  1:493512/1‑140 (MQ=38)

        aaGAATCCGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                                 <  2:989574/140‑1 (MQ=255)

               cGTATCTTCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgag                          <  2:525582/140‑1 (MQ=255)

                     ttCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc                    >  1:1374106/1‑140 (MQ=255)

                     ttCGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGATGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc                    >  2:1226029/1‑140 (MQ=255)

                       cGAGTGCCCACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg                  >  1:1224032/1‑140 (MQ=255)

                             cccACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc            >  2:451463/1‑140 (MQ=255)

                             cccACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc            >  1:1505065/1‑140 (MQ=255)

                             cccACACAGATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAc            >  2:918130/1‑140 (MQ=255)

                                     gATTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGa    >  2:1672307/1‑140 (MQ=255)

                                      aTTGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAc   >  1:827407/1‑140 (MQ=255)

                                       ttGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAcc  <  2:141881/140‑1 (MQ=255)

                                       ttGCCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGAcc  >  1:1265837/1‑140 (MQ=255)

GCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACC  >  NC_000913/2731238‑2731416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A