Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A32 F30 I1 R1	30	51.0	1723162	99.3%	1711099	139.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,219,615	C→T	D340D (GAC→GAT)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,219,615	0	C	T	100.0%	161.9 / NA	46	D340D (GAC→GAT)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (23/23); total (23/23)

GCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCAT  >  NC_000913/4219485‑4219747

gCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTcgcg                                                                                                                             <  2:544188/140‑1 (MQ=255)

  tACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGcgcc                                                                                                                           <  1:387454/140‑1 (MQ=255)

    cctcgAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGcgccgc                                                                                                                         >  2:380351/4‑140 (MQ=255)

       cgAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGa                                                                                                                      >  2:705590/1‑140 (MQ=255)

          atatCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAag                                                                                                                   <  1:421406/140‑1 (MQ=255)

                 gTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTc                                                                                                            <  1:125427/140‑1 (MQ=255)

                   ttATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTg                                                                                                          <  2:768477/140‑1 (MQ=255)

                   ttATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTg                                                                                                          <  2:119571/140‑1 (MQ=255)

                           aGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCAc                                                                                                  >  2:390747/1‑140 (MQ=255)

                             ggCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGt                                                                                                >  2:246537/1‑140 (MQ=255)

                               cTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTg                                                                                                          >  2:366968/1‑128 (MQ=255)

                               cTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTg                                                                                                          <  1:366968/128‑1 (MQ=255)

                                tGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCTAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCg                                                                                             >  2:62366/1‑140 (MQ=255)

                                tGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCg                                                                                             >  2:469119/1‑140 (MQ=255)

                                tGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCg                                                                                             >  1:764394/1‑140 (MQ=255)

                                tGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCg                                                                                             >  2:752500/1‑140 (MQ=255)

                                tGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCg                                                                                             <  1:246537/140‑1 (MQ=255)

                                  tAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTg                                                                                           >  2:287265/1‑140 (MQ=255)

                                   aaTGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                          <  1:156868/140‑1 (MQ=255)

                                   aaTGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGc                                                                                          <  1:287265/140‑1 (MQ=255)

                                      gAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTg                                                                                       <  1:705590/140‑1 (MQ=255)

                                       aGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGc                                                                                      >  2:27121/1‑140 (MQ=255)

                                        gCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCt                                                                                     >  2:169085/1‑140 (MQ=255)

                                         cAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTa                                                                                    >  2:605135/1‑140 (MQ=255)

                                          aGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTAt                                                                                   <  1:169085/140‑1 (MQ=255)

                                             tCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCaa                                                                                <  2:132410/140‑1 (MQ=255)

                                              cATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAAc                                                                               >  1:744888/1‑140 (MQ=255)

                                              cATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAAc                                                                               <  2:764727/140‑1 (MQ=255)

                                              cATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAAc                                                                               <  2:516344/140‑1 (MQ=255)

                                                    aGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCTAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGa                                                                         <  1:62366/140‑1 (MQ=255)

                                                    aGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGa                                                                         <  1:752500/140‑1 (MQ=255)

                                                      cgcCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGaaa                                                                       >  2:724538/1‑140 (MQ=255)

                                                      cgcCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGaaa                                                                       >  2:41264/1‑140 (MQ=255)

                                                        ccGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAaga                                                                     <  1:142489/140‑1 (MQ=255)

                                                                       aTGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTggg                                                      <  2:764394/140‑1 (MQ=255)

                                                                         ggTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGcc                                                    >  2:535286/1‑140 (MQ=255)

                                                                          gTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCg                                                   >  2:171356/1‑140 (MQ=255)

                                                                                  ggTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCgg                                           <  1:535286/140‑1 (MQ=255)

                                                                                   gggtTTTCGCTGCCGGGCTTTGATCGGCTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTGCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGa                                          <  2:218826/138‑1 (MQ=255)

                                                                                         aCGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCa                                    >  2:315650/1‑140 (MQ=255)

                                                                                         aCGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCa                                    >  2:238861/1‑140 (MQ=255)

                                                                                           gCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAgg                                  <  1:171356/140‑1 (MQ=255)

                                                                                                                     aaGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAg        <  1:315650/140‑1 (MQ=255)

                                                                                                                      aGTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGc       >  2:57644/1‑140 (MQ=255)

                                                                                                                       gTCATCAAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCg      <  1:469119/140‑1 (MQ=255)

                                                                                                                           tcaAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCat  >  2:504523/1‑140 (MQ=255)

                                                                                                                           tcaAAGATAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGGTTGAAAACTTTGTGCTGGAGAAGCGGCat  >  2:128785/1‑140 (MQ=255)

GCTACCGCGAATATCTCGTTTATCTACAGGGCTGTAATGAGCAGTTCATTGAAGCGCCGGGTATTCGTGGAATGGTGATGTTGGTGTTTACGCTGCCGGGCTTTGATCGGGTATTCAAAGTCATCAAAGACAGGTTCGCGCCGCAGAAAGAGATGTCTGCCGCTCACGTTCGTGCCTGCTATCAACTGGTGAAAGAGCACGATCGCGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCAT  >  NC_000913/4219485‑4219747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A