breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 173,109 C→A L550M (CTG→ATG)  fhuB → iron(III) hydroxamate ABC transporter membrane subunit
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 302,016 G→T V186V (GTC→GTA paoA ← aldehyde dehydrogenase, Fe‑S subunit
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
JC JC 1,217,386 IS1 (–) +8 bp coding (59‑66/1524 nt) pdeG → putative c‑di‑GMP phosphodiesterase PdeG
RA 1,230,822 G→T S19I (AGC→ATC)  umuD → DNA polymerase V protein UmuD
RA 1,658,572 G→T M168I (ATG→ATT ynfE → putative selenate reductase YnfE
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,820,775 G→T A147E (GCG→GAG)  chbC ← N,N'‑diacetylchitobiose‑specific PTS enzyme IIC component
RA 1,839,491 G→C A9P (GCG→CCG)  ynjE → molybdopterin synthase sulfurtransferase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,967,672 C→A G277V (GGG→GTG)  cheB ← protein‑glutamate methylesterase/protein glutamine deamidase
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,312,221 G→T P177T (CCA→ACA)  ompC ← outer membrane porin C
RA 2,363,183 G→T P151T (CCC→ACC)  yfaY ← IPR008135 CinA family protein YfaY
RA 2,616,809 C→A P239Q (CCG→CAG)  bepA → beta‑barrel assembly‑enhancing protease
RA 2,762,018 C→A R508L (CGT→CTT)  abpB ← CP4‑57 prophage; putative helicase YfjK
RA 2,898,944 G→T R159R (CGA→AGA)  ygcU ← putative FAD‑containing dehydrogenase
RA 3,135,446 G→T A309E (GCA→GAA)  pitB ← metal phosphate:H(+) symporter PitB
RA 3,204,883 C→A L64M (CTG→ATG)  plsY → putative glycerol‑3‑phosphate acyltransferase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,219,615 C→T D340D (GAC→GAT aceK → isocitrate dehydrogenase kinase/phosphatase
RA 4,244,141 C→A R312L (CGC→CTC)  malF ← maltose ABC transporter membrane subunit MalF
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,382,280 G→T A13A (GCC→GCA frdA ← fumarate reductase flavoprotein subunit
RA 4,505,167 C→A G370G (GGC→GGA yjhB → putative sialic acid transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102950 3104063 1114 13 [12] [12] 14 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424233 3424526–3424239 7–757 13 [11] [11] 13 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)54 (1.090) 34/268 0.4 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =100 (1.970)78 (1.550) 48/274 0.1 60.9% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 1 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)52 (1.020) 38/276 0.3 98.1% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA