Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F73 I1 R1	29	140.0	4785556	99.3%	4752057	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,696,083	C→A	D35Y (GAT→TAT)	yhjR ←	PF10945 family protein YhjR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,696,083	0	C	A	99.4%	600.2 / ‑3.3	175	D35Y (GAT→TAT)	yhjR	PF10945 family protein YhjR
Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (75/99); total (75/100)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.07e-01

Only 100 of 175 total aligned reads displayed.

CACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACC  >  NC_000913/3695947‑3696215

cACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAat                                                                                                                                   >  2:337766/1‑140 (MQ=255)

  cGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAatat                                                                                                                                 <  1:1533937/140‑1 (MQ=255)

   gCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATc                                                                                                                                >  1:786245/1‑140 (MQ=255)

    ccTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCa                                                                                                                               <  1:1174621/140‑1 (MQ=255)

     cTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAg                                                                                                                              >  2:2098467/1‑140 (MQ=255)

               ccccTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGaaaa                                                                                                                    >  1:1429109/1‑140 (MQ=255)

                 ccTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATg                                                                                                                  >  1:355294/1‑140 (MQ=255)

                 ccTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATg                                                                                                                  >  1:588286/1‑140 (MQ=255)

                  cTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGc                                                                                                                 >  1:1003813/1‑140 (MQ=255)

                  cTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGc                                                                                                                 <  2:1094820/140‑1 (MQ=255)

                   tGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGcc                                                                                                                <  2:1860831/140‑1 (MQ=255)

                   tGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGcc                                                                                                                <  2:2201700/140‑1 (MQ=255)

                    gCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCt                                                                                                               >  2:1635932/1‑140 (MQ=255)

                       aTCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCt                                                                                                            >  2:286291/1‑140 (MQ=255)

                         cccAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCttt                                                                                                          >  2:1017720/1‑140 (MQ=255)

                         cccAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCttt                                                                                                          >  1:2106152/1‑140 (MQ=255)

                         cccAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCttt                                                                                                          >  1:2314620/1‑140 (MQ=255)

                         cccAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCttt                                                                                                          >  1:1702774/1‑140 (MQ=255)

                           cAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTaa                                                                                                        >  2:993297/1‑140 (MQ=255)

                             gTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACg                                                                                                      >  2:1506299/1‑140 (MQ=255)

                             gTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACg                                                                                                      <  1:993297/140‑1 (MQ=255)

                              tACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGc                                                                                                     <  1:1506299/140‑1 (MQ=255)

                               aCGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGcc                                                                                                    >  2:637471/1‑140 (MQ=255)

                               aCGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGcc                                                                                                    <  2:60958/140‑1 (MQ=255)

                                cGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCca                                                                                                   >  2:2392336/1‑140 (MQ=255)

                                cGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCca                                                                                                   <  2:556503/140‑1 (MQ=255)

                                  gCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGcc                                                                                                                >  1:1202051/1‑125 (MQ=255)

                                  gCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGcc                                                                                                                <  2:1202051/125‑1 (MQ=255)

                                  gCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCcaca                                                                                                 >  2:2047120/1‑140 (MQ=255)

                                  gCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCcaca                                                                                                 >  1:392628/1‑140 (MQ=255)

                                  gCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCcaca                                                                                                 >  1:392627/1‑140 (MQ=255)

                                   ccATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACaa                                                                                                >  2:1696373/1‑140 (MQ=255)

                                     aTTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAata                                                                                              <  2:113737/140‑1 (MQ=255)

                                      ttCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAatat                                                                                             >  1:900362/1‑140 (MQ=255)

                                      ttCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAatat                                                                                             >  2:2235024/1‑140 (MQ=255)

                                      ttCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAatat                                                                                             >  1:1589984/1‑140 (MQ=255)

                                        cACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCa                                                                                           <  1:1635932/140‑1 (MQ=255)

                                        cACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCa                                                                                           <  2:1568819/140‑1 (MQ=255)

                                          ccAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCAtt                                                                                         <  2:968366/140‑1 (MQ=255)

                                          ccAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCAtt                                                                                         <  1:1380563/140‑1 (MQ=255)

                                             aTCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGc                                                                                                     <  2:1150406/125‑1 (MQ=255)

                                             aTCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTg                                                                                      >  2:1343951/1‑140 (MQ=255)

                                              tCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTgg                                                                                     <  2:1936051/140‑1 (MQ=255)

                                              tCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTgg                                                                                     <  1:2098467/140‑1 (MQ=255)

                                              tCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTgg                                                                                     <  1:1087359/140‑1 (MQ=255)

                                              tCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTgg                                                                                     <  2:1429109/140‑1 (MQ=255)

                                              tCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTgg                                                                                     <  1:481582/140‑1 (MQ=255)

                                              tCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTgg                                                                                     <  2:2106152/140‑1 (MQ=255)

                                               ccccTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAat                                                                                               <  1:1300773/129‑1 (MQ=255)

                                               ccccTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGa                                                                                    <  1:1009028/140‑1 (MQ=255)

                                                 ccTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAg                                                                                  <  1:117631/140‑1 (MQ=255)

                                                    aCTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGata                                                                               <  1:286291/140‑1 (MQ=255)

                                                        ttgttgCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGc                                                                           <  1:2353749/140‑1 (MQ=255)

                                                        ttgttgCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGc                                                                           <  1:403574/140‑1 (MQ=255)

                                                         tgttgCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGcc                                                                          <  1:2222738/140‑1 (MQ=255)

                                                            tgCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCAtt                                                                                         >  1:840116/1‑122 (MQ=255)

                                                              cgcAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATcg                                                                     <  2:131670/140‑1 (MQ=255)

                                                               gcAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATcgc                                                                    >  1:2202846/1‑140 (MQ=255)

                                                               gcAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATcgc                                                                    >  1:599455/1‑140 (MQ=255)

                                                               gcAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATcgc                                                                    >  1:135924/1‑140 (MQ=255)

                                                                cAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATcgcg                                                                   >  1:832195/1‑140 (MQ=255)

                                                                cAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATcgcg                                                                   >  2:1761260/1‑140 (MQ=255)

                                                                cAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATcgcg                                                                   <  2:1847591/140‑1 (MQ=255)

                                                                  aaCTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCggg                                                                 >  2:1622883/1‑140 (MQ=255)

                                                                   aCTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGa                                                                <  1:533510/140‑1 (MQ=255)

                                                                     tctGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATc                                                              <  1:2183394/140‑1 (MQ=255)

                                                                     tctGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATc                                                              <  2:421293/140‑1 (MQ=255)

                                                                      ctGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCa                                                             <  1:2047120/140‑1 (MQ=255)

                                                                       tGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAg                                                            <  2:355294/140‑1 (MQ=255)

                                                                        gCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAgg                                                           <  2:87393/140‑1 (MQ=255)

                                                                         ccAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGc                                                          <  2:832195/140‑1 (MQ=255)

                                                                         ccAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGc                                                          <  2:1809811/140‑1 (MQ=255)

                                                                          cAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAGGATATAGCCTATCGCGGGATCAGGCa                                                         <  1:2392336/140‑1 (MQ=255)

                                                                          cAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCa                                                         <  1:1868545/140‑1 (MQ=255)

                                                                          cAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCa                                                         <  2:2314620/140‑1 (MQ=255)

                                                                            gCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCaga                                                       <  1:2235024/140‑1 (MQ=255)

                                                                            gCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCaga                                                       <  2:851606/140‑1 (MQ=255)

                                                                             cAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCagag                                                      >  1:1522756/1‑140 (MQ=255)

                                                                                        gTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCa                                           <  2:1158500/140‑1 (MQ=255)

                                                                                              aTGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTa                                     <  2:135924/140‑1 (MQ=255)

                                                                                                   gcgGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCAt                                <  1:637471/140‑1 (MQ=255)

                                                                                                    cgGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCa                                           <  2:795726/128‑1 (MQ=255)

                                                                                                    cgGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCa                                           >  1:795726/1‑128 (MQ=255)

                                                                                                    cgGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCAtt                               <  1:1696373/140‑1 (MQ=255)

                                                                                                    cgGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCAtt                               >  2:496589/1‑140 (MQ=255)

                                                                                                    cgGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCAtt                               >  1:208385/1‑140 (MQ=255)

                                                                                                    cgGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCAtt                               >  2:390826/1‑140 (MQ=255)

                                                                                                    cgGCCAACTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCAtt                               >  1:1900821/1‑140 (MQ=255)

                                                                                                           cTGTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTg                        <  2:2148634/140‑1 (MQ=255)

                                                                                                             gTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGAt                      <  2:443626/140‑1 (MQ=255)

                                                                                                             gTTCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGAt                      <  2:1900821/140‑1 (MQ=255)

                                                                                                              ttCGCGTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATc                     >  1:610078/1‑140 (MQ=255)

                                                                                                                cgcgTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATccc                   <  2:2345095/140‑1 (MQ=255)

                                                                                                                cgcgTTGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATccc                   <  1:1761260/140‑1 (MQ=255)

                                                                                                                     tGGGAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGa              <  2:1003813/140‑1 (MQ=255)

                                                                                                                       ggAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGaaa            <  2:392628/140‑1 (MQ=255)

                                                                                                                       ggAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGaaa            <  1:1844330/140‑1 (MQ=255)

                                                                                                                       ggAAATATCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGaaa            <  1:496589/140‑1 (MQ=255)

                                                                                                                           atatCGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAac        <  1:1151493/140‑1 (MQ=255)

                                                                                                                               cGGCATAATAAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAaccgta    <  1:2028595/140‑1 (MQ=255)

CACGCCTCCCCGCACCCCCTGCTATCCCAGTACGGCCATTCACCAATCCCCTACTTTTGTTGCGCAAACTCTGCCAGCAACGGCCAGCGTTTTAATGCCGCGGCCAACTGTTCGCGTTGGGAAATATCGGCATAATCAATATCAGGCAGTGAAAATGCCTGCTTTAACGCCACAATATCATTCTGGAAGATATAGCCTATCGCGGGATCAGGCAGAGTATCTGGTTCATTGTTATTCATTTTGCTTGATCCCTATGAAATAACCGTACC  >  NC_000913/3695947‑3696215

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A