breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 513,935 (A)7→8 intergenic (+67/‑58) ybaT → / → cueR putative transporter YbaT/DNA‑binding transcriptional dual regulator CueR
RA 550,189 C→A A188E (GCG→GAG)  ylbF → DUF2877 domain‑containing protein YlbF
RA 871,543 C→A R193S (CGC→AGC)  gsiC → glutathione ABC transporter membrane subunit GsiC
RA 886,888 G→T T151K (ACG→AAG)  ybjJ ← inner membrane protein YbjJ
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,077,913 G→T P324T (CCG→ACG)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,103,150 A→G I16T (ATC→ACC)  csgD ← DNA‑binding transcriptional dual regulator CsgD
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,624,927 G→A L628L (CTG→CTA ppk → polyphosphate kinase
RA 2,731,130 T→C noncoding (28/1542 nt) rrsG ← 16S ribosomal RNA
RA 3,067,537 G→T P182T (CCG→ACG)  yggE ← uncharacterized protein YggE
RA 3,100,766 C→A intergenic (‑38/+138) ansB ← / ← yggN L‑asparaginase 2/putative EcfF
RA 3,223,159 G→T R176L (CGC→CTC)  ebgA → evolved beta‑D‑galactosidase subunit alpha
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,608,061 C→A S537R (AGC→AGA zntA → Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
RA 3,696,083 C→A D35Y (GAT→TAT)  yhjR ← PF10945 family protein YhjR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,935,539 C→A G84G (GGC→GGA rbsC → ribose ABC transporter membrane subunit
RA 4,202,066 G→A Q47Q (CAG→CAA zraS → sensory histidine kinase ZraS
RA 4,222,600 G→T intergenic (‑113/+204) arpA ← / ← iclR regulator of acetyl CoA synthetase/DNA‑binding transcriptional repressor IclR
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,412,516 C→A L44I (CTA→ATA)  yjfM → DUF1190 domain‑containing protein YjfM
RA 4,552,043 C→A F136L (TTC→TTA uxuA → D‑mannonate dehydratase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102942 3104055 1114 38 [37] [37] 39 [mutY] [mutY]
* * ÷ NC_000913 3423751–3424234 3424545–3424238 5–795 38 [36] [35] 41 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =113 (0.800)75 (0.600) 54/244 0.6 42.8% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 101 (0.810)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 1207805113 (0.800)38 (0.300) 32/244 2.0 27.5% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 101 (0.810)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)204 (1.490) 96/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =160 (1.130)197 (1.410) 80/274 0.2 70.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 5 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)183 (1.300) 84/276 0.1 97.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 4 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA