Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A15 F73 I1 R1	29	140.0	4785556	99.3%	4752057	139.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,202,066	G→A	Q47Q (CAG→CAA)	zraS →	sensory histidine kinase ZraS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,202,066	0	G	A	100.0%	702.8 / NA	193	Q47Q (CAG→CAA)	zraS	sensory histidine kinase ZraS
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (97/96); total (97/96)

Only 100 of 194 total aligned reads displayed.

TTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGTTGTGGT  >  NC_000913/4201933‑4202202

   tgtaaCGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTg                                                                                                                                 <  1:802214/137‑1 (MQ=255)

   tGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTg                                                                                                                                 >  2:1344236/1‑140 (MQ=255)

   tGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTg                                                                                                                                 <  1:1473775/140‑1 (MQ=255)

        cGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGaaaa                                                                                                                            >  2:2290155/1‑140 (MQ=255)

             tAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTa                                                                                                                       >  2:978072/1‑140 (MQ=255)

             tAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTa                                                                                                                       >  1:2261987/1‑140 (MQ=255)

                  aCTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAAtgtg                                                                                                                  >  1:192004/1‑140 (MQ=255)

                    tCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCt                                                                                                                >  1:312662/1‑140 (MQ=255)

                    tCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCt                                                                                                                >  2:2119151/1‑140 (MQ=255)

                    tCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCt                                                                                                                <  2:2189693/140‑1 (MQ=255)

                    tCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCt                                                                                                                >  2:1010139/1‑140 (MQ=255)

                    tCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCt                                                                                                                >  1:2132889/1‑140 (MQ=255)

                    tCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCt                                                                                                                >  1:2078563/1‑140 (MQ=255)

                    tCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCt                                                                                                                >  1:1346392/1‑140 (MQ=255)

                      cTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTa                                                                                                              >  1:1427036/1‑140 (MQ=255)

                       ttAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTAt                                                                                                             <  1:1003071/140‑1 (MQ=255)

                       ttAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTAt                                                                                                             >  1:1340908/1‑140 (MQ=255)

                         aGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAgg                                                                                                                         >  1:2033741/1‑126 (MQ=255)

                         aGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAgg                                                                                                                         <  2:2033741/126‑1 (MQ=255)

                         aGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATcc                                                                                                           >  1:1126984/1‑140 (MQ=255)

                         aGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATcc                                                                                                           <  1:83267/140‑1 (MQ=255)

                          gCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCcg                                                                                                          <  1:1297293/140‑1 (MQ=255)

                          gCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCcg                                                                                                          <  2:2143224/140‑1 (MQ=255)

                          gCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCcg                                                                                                          <  2:165507/140‑1 (MQ=255)

                            tAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCcgcg                                                                                                        >  1:1245168/1‑140 (MQ=255)

                            tAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCcgcg                                                                                                        <  1:1051203/140‑1 (MQ=255)

                            tAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCcgcg                                                                                                        <  2:1427036/140‑1 (MQ=255)

                             aaaTGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCgcgc                                                                                                       >  2:1409346/1‑140 (MQ=255)

                              aaTGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGAAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGct                                                                                                      >  1:2059908/1‑140 (MQ=255)

                              aaTGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGCGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGct                                                                                                      >  1:86541/1‑140 (MQ=255)

                                tGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGctct                                                                                                    >  2:1941052/1‑140 (MQ=255)

                                  gTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTgg                                                                                                  <  2:2132889/140‑1 (MQ=255)

                                     aaGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGt                                                                                               >  1:884914/1‑140 (MQ=255)

                                     aaGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGt                                                                                               >  2:733110/1‑140 (MQ=255)

                                      aGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTc                                                                                              <  2:685731/140‑1 (MQ=255)

                                            aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                        >  2:581194/1‑140 (MQ=255)

                                            aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                        <  1:222713/140‑1 (MQ=255)

                                            aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                        >  1:1309596/1‑140 (MQ=255)

                                            aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                        <  1:769355/140‑1 (MQ=255)

                                            aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                        >  1:1569971/1‑140 (MQ=255)

                                            aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                        <  2:341070/140‑1 (MQ=255)

                                            aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                        >  1:642902/1‑140 (MQ=255)

                                            aTCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                        <  1:161728/140‑1 (MQ=255)

                                               ctccCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCcg                                                                                     <  2:175818/140‑1 (MQ=255)

                                                  cccGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGt                                                                                  <  1:978072/140‑1 (MQ=255)

                                                  cccGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGt                                                                                  <  1:1941052/140‑1 (MQ=255)

                                                     gTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAg                                                                                        >  2:1607534/1‑131 (MQ=255)

                                                       ggTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAggga                                                                             <  1:1879548/140‑1 (MQ=255)

                                                       ggTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAggga                                                                             >  2:1519946/1‑140 (MQ=255)

                                                       ggTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAggga                                                                             <  1:581194/140‑1 (MQ=255)

                                                               ttgGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAgggatggggatg                                                                     <  2:1056997/140‑1 (MQ=255)

                                                                  ggCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGa                                                                  <  2:884914/140‑1 (MQ=255)

                                                                  ggCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGa                                                                  >  2:2117138/1‑140 (MQ=255)

                                                                    cTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAAt                                                                <  1:1699882/140‑1 (MQ=255)

                                                                      ggtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGATAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGc                                                              >  2:2376757/1‑140 (MQ=255)

                                                                       gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCa                                                             <  2:1309596/140‑1 (MQ=255)

                                                                       gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCa                                                             <  2:192004/140‑1 (MQ=255)

                                                                       gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCa                                                             <  2:2163282/140‑1 (MQ=255)

                                                                       gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCa                                                             <  1:733110/140‑1 (MQ=255)

                                                                       gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCa                                                             >  1:2163282/1‑140 (MQ=255)

                                                                       gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCa                                                             >  2:297666/1‑140 (MQ=255)

                                                                       gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCa                                                             >  1:424274/1‑140 (MQ=255)

                                                                       gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCa                                                             >  1:2301104/1‑140 (MQ=255)

                                                                       gtggGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGATAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCa                                                             <  1:2376757/140‑1 (MQ=255)

                                                                         gggATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCAcc                                                           <  1:2012387/140‑1 (MQ=255)

                                                                         gggATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCAcc                                                           <  2:1698024/140‑1 (MQ=255)

                                                                         gggATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCAcc                                                           <  2:2261987/140‑1 (MQ=255)

                                                                                 ttGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAAt                                                                >  2:181608/1‑127 (MQ=255)

                                                                                 ttGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAg                                                   >  1:1536839/1‑140 (MQ=255)

                                                                                     ggTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAAc                                               >  2:815969/1‑140 (MQ=255)

                                                                                        aaCTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAgg                                            <  2:565281/140‑1 (MQ=255)

                                                                                         aCTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGgc                                           <  2:1191354/140‑1 (MQ=255)

                                                                                         aCTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGgc                                           >  1:1760116/1‑140 (MQ=255)

                                                                                         aCTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGgc                                           <  2:1126984/140‑1 (MQ=255)

                                                                                         aCTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGgc                                           <  1:1257210/140‑1 (MQ=255)

                                                                                          cTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGgcg                                          >  2:1858529/1‑140 (MQ=255)

                                                                                          cTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGgcg                                          <  1:1072999/140‑1 (MQ=255)

                                                                                           tgtgATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGgcgc                                         <  1:1890322/140‑1 (MQ=255)

                                                                                           tgtgATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGgcgc                                         <  1:1519946/140‑1 (MQ=255)

                                                                                               aTTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCt                                     >  1:556933/1‑140 (MQ=255)

                                                                                                ttCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTg                                    <  2:1725874/140‑1 (MQ=255)

                                                                                                 tCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACGGCAACAGGCGCTTCTgg                                   >  1:120912/1‑140 (MQ=255)

                                                                                                   gTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGaa                                 >  1:453830/1‑140 (MQ=255)

                                                                                                   gTGATTATGGGCGGGCAAGCGAGGCAGACCACCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGaa                                 <  1:1837328/140‑1 (MQ=255)

                                                                                                              cgggcAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCggg                      <  1:753111/140‑1 (MQ=255)

                                                                                                              cgggcAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCggg                      >  2:1474267/1‑140 (MQ=255)

                                                                                                              cgggcAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCggg                      >  1:2143719/1‑140 (MQ=255)

                                                                                                               gggcAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGa                     <  1:211906/140‑1 (MQ=255)

                                                                                                               gggcAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGa                     <  1:1500018/140‑1 (MQ=255)

                                                                                                                 gcAAGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACa                   >  1:1005368/1‑140 (MQ=255)

                                                                                                                   aaGCGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGc                 <  1:2134854/140‑1 (MQ=255)

                                                                                                                      cGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCgg              <  1:1746473/140‑1 (MQ=255)

                                                                                                                      cGAGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCgg              <  1:1409346/140‑1 (MQ=255)

                                                                                                                        aGGCAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGa            <  2:2059908/140‑1 (MQ=255)

                                                                                                                           cAGACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAgtg         >  2:517038/1‑140 (MQ=255)

                                                                                                                             gACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGtgtt       <  1:517038/140‑1 (MQ=255)

                                                                                                                             gACCGCCAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGtgtt       <  2:642902/140‑1 (MQ=255)

                                                                                                                                  ccAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGTTGTGGt  <  2:1569971/140‑1 (MQ=255)

                                                                                                                                  ccAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGTTGTGGt  <  2:1520485/140‑1 (MQ=255)

                                                                                                                                  ccAAGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGTTGTGGt  <  1:252083/140‑1 (MQ=255)

TTATGCAACGTTCTAAAGACTCCTTAGCTAAATGGTTAAGCGCGATCCTCCCCGTGGTCATTGTTGGGCTGGTGGGATTGTTTGCGGTAACTGTGATTCGTGATTATGGGCGGGCAAGCGAGGCAGACCGCCAGGCATTACTGGAAAAAGGTAATGTGCTTATCCGCGCTCTGGAGTCGGGAAGCCGCGTAGGGATGGGGATGCGAATGCACCATGTACAGCAACAGGCGCTTCTGGAAGAGATGGCGGGACAGCCGGGAGTGTTGTGGT  >  NC_000913/4201933‑4202202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A