Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A12 F73 I1 R1	29	53.4	1802238	99.2%	1787820	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,390,169	G→T	R217I (AGA→ATA)	aaeR →	LysR‑type transcriptional regulator AaeR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,390,169	0	G	T	100.0%	160.7 / NA	46	R217I (AGA→ATA)	aaeR	LysR‑type transcriptional regulator AaeR
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (26/20); total (26/20)

TCTCACACAATACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCCTGCTGCCGCGTTACCAGTCA  >  NC_000913/3390032‑3390305

tctcACACAATACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAatat                                                                                                                                        >  2:272549/1‑140 (MQ=255)

      acaATACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGa                                                                                                                                  >  2:849877/1‑140 (MQ=255)

          tACGGCATACGGAAAAACCCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTaa                                                                                                                              <  2:299410/140‑1 (MQ=255)

          tACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTaa                                                                                                                              >  2:73139/1‑140 (MQ=255)

          tACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTaa                                                                                                                              >  1:517092/1‑140 (MQ=255)

              gCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGAt                                                                                                                          <  2:264919/140‑1 (MQ=255)

              gCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGAt                                                                                                                          >  2:828020/1‑140 (MQ=255)

                aTACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATcc                                                                                                                        >  1:371935/1‑140 (MQ=255)

                  aCCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATAttt                                                                                                                                      >  1:788590/1‑124 (MQ=255)

                  aCCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATAttt                                                                                                                                      <  2:788590/124‑1 (MQ=255)

                  aCCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGa                                                                                                                      <  1:501225/140‑1 (MQ=255)

                                               attcatGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCgggg                                                                                         >  1:354489/1‑139 (MQ=255)

                                                    tGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCgg                                                                                    <  1:539445/140‑1 (MQ=255)

                                                     ggCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCggg                                                                                   <  1:254847/140‑1 (MQ=255)

                                                       cTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGAt                                                                                 >  1:440710/1‑140 (MQ=255)

                                                          gAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGc                                                                              >  1:560170/1‑140 (MQ=255)

                                                               cAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACg                                                                         <  1:590068/140‑1 (MQ=255)

                                                                 gCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTg                                                                       <  2:174208/140‑1 (MQ=255)

                                                                  cGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCCGGATCGCCTACGTGc                                                                      >  1:305800/1‑140 (MQ=255)

                                                                   gTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGcc                                                                     <  2:58391/140‑1 (MQ=255)

                                                                   gTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGcc                                                                     <  1:22817/140‑1 (MQ=255)

                                                                     gCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGGGCCGGGATCGCCTACGTGCCGc                                                                   >  2:186833/1‑140 (MQ=255)

                                                                     gCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGGGCCGGGAATGCCTACGGGCCGc                                                                   >  1:137086/1‑140 (MQ=255)

                                                                       ggCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTg                                                                 >  1:136721/1‑140 (MQ=255)

                                                                          ccGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGAtg                                                              <  1:849877/140‑1 (MQ=255)

                                                                                aTGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTg                                                        <  2:136721/140‑1 (MQ=255)

                                                                                         aaCTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACgag                                               >  2:673305/1‑140 (MQ=255)

                                                                                           cTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGAt                                             <  2:517092/140‑1 (MQ=255)

                                                                                             gATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCa                                           >  1:619108/1‑140 (MQ=255)

                                                                                             gATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGGGATCAACGAGATCa                                           >  2:474173/1‑140 (MQ=255)

                                                                                              aTCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCaa                                          >  2:425634/1‑140 (MQ=255)

                                                                                                cGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATc                                        >  2:427862/1‑140 (MQ=255)

                                                                                                   aCCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTg                                     <  1:858794/140‑1 (MQ=255)

                                                                                                   aCCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTg                                     <  2:678135/140‑1 (MQ=255)

                                                                                                   aCCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGGGCCGGGGTCGCCTACGTGCCGCTGATGTGGGGGATCAACGAGATCAATCGTg                                     >  2:241203/1‑140 (MQ=255)

                                                                                                   aCCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGGGCCGGGATCGCCTACGTGCCGCTGATGTGGGGGATCAACGAGATCAATCGTg                                     >  2:208427/1‑140 (MQ=255)

                                                                                                   aCCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGGGCCGGGATCGCCCACGTGCCGCTGGTGTGGGGGATCAACGAGATCAATCGTg                                     >  2:381024/1‑140 (MQ=255)

                                                                                                            gATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGa                                 >  1:667161/1‑135 (MQ=255)

                                                                                                            gATCTCGACTCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGa                                 <  2:667161/135‑1 (MQ=255)

                                                                                                                     tCGCCTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGGTCGCCTACGTGCCGCTGATGTGGGGGATCAACGAGATCAATCGTGGGGGGCTGGGGATCctgc                   >  2:616960/1‑140 (MQ=255)

                                                                                                                        ccTGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCCtgctgc                <  1:673305/140‑1 (MQ=255)

                                                                                                                          tGATCCCACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCCTGCTGCcg              <  1:560844/140‑1 (MQ=255)

                                                                                                                              cccACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCCTTCTGCCGCGtt          >  1:188478/1‑140 (MQ=255)

                                                                                                                               ccACAAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCCTGCTGCCGCGTTa         <  1:155871/140‑1 (MQ=255)

                                                                                                                                 acaAGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCCTGCTGCCGCGTTAcc       >  2:699850/1‑140 (MQ=255)

                                                                                                                                    aGGAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGGGATCAACGAGATCAATCGTGGGGAGCTGGAGATCCTGCTGCCGCGTTACCAGt    >  1:495387/1‑140 (MQ=255)

                                                                                                                                      gAATATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCCTGCTGCCGCGTTACCAGTCa  <  1:425634/140‑1 (MQ=255)

TCTCACACAATACGGCATACCGGAAAAACCCGCCGATTTGAGTAGTCATTCATGGCTTGAATACAGCGTGCGGCCCGACAATGAATTTGAACTGATCGCACCGGAAGGGATCTCGACTCGCCTGATCCCACAAGGAAGATTTGTGACTAATGATCCGATGACGCTGGTGCGCTGGCTGACGGCGGGTGCCGGGATCGCCTACGTGCCGCTGATGTGGGTGATCAACGAGATCAATCGTGGGGAGCTGGAGATCCTGCTGCCGCGTTACCAGTCA  >  NC_000913/3390032‑3390305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A