breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 129,135 G→T V408V (GTG→GTT lpd → lipoamide dehydrogenase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,359,925 G→T N328K (AAC→AAA puuA ← glutamate‑putrescine ligase
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,015,254 G→T E125D (GAG→GAT fliG → flagellar motor switch protein FliG
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,425,329 G→T V196V (GTC→GTA hisQ ← lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisQ
RA 2,470,789 G→T intergenic (+327/+288) yfdI → / ← yfdK CPS‑53 (KpLE1) prophage; serotype specific glucosyl transferase/CPS‑53 (KpLE1) prophage; putative tail fiber assembly protein YfdK
RA 2,547,948 C→A P426T (CCG→ACG)  murP → N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
RA 2,611,955 C→A A19E (GCG→GAG)  hyfR → DNA‑binding transcriptional activator HyfR
RA 2,867,169 A→G I128T (ATC→ACC)  rpoS ← RNA polymerase, sigma S (sigma 38) factor
RA 2,974,644 G→T T457T (ACC→ACA aas ← acyltransferase
RA 3,008,155 C→A Q216K (CAA→AAA)  ygeX → 2,3‑diaminopropionate ammonia‑lyase
RA 3,232,125 C→A A154E (GCA→GAA)  fadH → 2,4‑dienoyl‑CoA reductase
RA 3,314,889 C→A A376S (GCG→TCG)  infB ← translation initiation factor IF‑2
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,390,169 G→T R217I (AGA→ATA)  aaeR → LysR‑type transcriptional regulator AaeR
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,604,429 C→A R13S (CGC→AGC)  rsmD → 16S rRNA m(2)G966 methyltransferase
RA 3,787,221 C→A G128G (GGC→GGA envC → murein hydrolase activator EnvC
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,947,041 C→A intergenic (+9/+87) trpT → / ← hdfR tRNA‑Trp/DNA‑binding transcriptional dual regulator HdfR
RA 3,993,027 C→A G625G (GGC→GGA cyaA → adenylate cyclase
RA 4,092,290 G→T A26D (GCC→GAC)  frvB ← putative PTS enzyme IIBC component FrvB
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102944 3104055 1112 15 [13] [13] 14 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424234 3424525–3424239 6–756 15 [13] [13] 16 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =22 (0.410)68 (1.450) 45/246 0.1 78.4% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 18 (0.380)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780522 (0.410)30 (0.640) 23/246 0.9 61.6% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 18 (0.380)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)69 (1.340) 48/270 0.1 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =64 (1.200)79 (1.500) 56/276 0.0 70.7% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.040)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 3583371 =NA (NA)101 (1.900) 66/278 0.0 NA pseudogene (334/390 nt) yrhA putative uncharacterized protein YrhA
?NC_000913 = 3584195 NA (NA)noncoding (768/768 nt) IS1 repeat region
* ? NC_000913 = 35833794 (0.080)69 (1.300) 51/278 0.1 94.5% pseudogene (342/390 nt) yrhA putative uncharacterized protein YrhA
?NC_000913 3583428 = NA (NA)noncoding (1/768 nt) IS1 repeat region
* ? NC_000913 = 3653230NA (NA)74 (1.390) 49/278 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 0 (0.000)coding (3907/4134 nt) rhsA rhs element protein RhsA