Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A12 F73 I1 R1 29 53.4 1802238 99.2% 1787820 139.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,787,221 C→A G128G (GGC→GGA)  envC → murein hydrolase activator EnvC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,787,2210CA100.0% 166.6 / NA 47G128G (GGC→GGA) envCmurein hydrolase activator EnvC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/23);  total (24/23)

CGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAG  >  NC_000913/3787084‑3787354
                                                                                                                                         |                                                                                                                                     
cgcgAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGgaga                                                                                                                                     >  2:58767/1‑140 (MQ=255)
cgcgAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGgaga                                                                                                                                     >  2:148364/1‑140 (MQ=255)
cgcgAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGgaga                                                                                                                                     >  1:880158/1‑140 (MQ=255)
cgcgAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGgaga                                                                                                                                     >  1:563630/1‑140 (MQ=255)
  cgAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGc                                                                                                                                   >  2:529691/1‑140 (MQ=255)
   gAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCa                                                                                                                                  <  1:688931/140‑1 (MQ=255)
                      atcaaCTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGAtt                                                                                                               >  2:116254/1‑140 (MQ=255)
                      atcaaCTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGAtt                                                                                                               >  1:203686/1‑140 (MQ=255)
                          aCTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCa                                                                                                           <  1:566253/140‑1 (MQ=255)
                              aaTAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCgg                                                                                                       <  1:141997/140‑1 (MQ=255)
                               aTAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGt                                                                                                      >  1:246434/1‑140 (MQ=255)
                                 aaaCAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTga                                                                                                    >  1:90495/1‑140 (MQ=255)
                                       aTTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAg                                                                                              <  1:323528/140‑1 (MQ=255)
                                       aTTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAg                                                                                              <  2:90495/140‑1 (MQ=255)
                                       aTTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAg                                                                                              <  1:88483/140‑1 (MQ=255)
                                        ttGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGc                                                                                             >  2:77170/1‑140 (MQ=255)
                                        ttGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGc                                                                                             >  1:608757/1‑140 (MQ=255)
                                          gatgagatgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCa                                                                                           >  1:511743/1‑140 (MQ=255)
                                            tgagatgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGTCAGc                                                                                         >  1:617375/1‑140 (MQ=255)
                                            tgagatgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGc                                                                                         >  2:819585/1‑140 (MQ=255)
                                                 tgaACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGa                                                                                    >  1:690751/1‑140 (MQ=255)
                                                     cgcgTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGc                                                                                >  2:51671/1‑140 (MQ=255)
                                                     cgcgTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGc                                                                                >  1:222336/1‑140 (MQ=255)
                                                     cgcgTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGc                                                                                >  1:47499/1‑140 (MQ=255)
                                                         tCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGttt                                                                            >  1:251932/1‑140 (MQ=255)
                                                          cGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTa                                                                           >  2:345939/1‑140 (MQ=255)
                                                          cGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTa                                                                           <  2:319826/140‑1 (MQ=255)
                                                              tGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAgg                                                                       <  2:880158/140‑1 (MQ=255)
                                                                   aaCTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGTGTTTACAGGCTTAt                                                                  <  2:161582/140‑1 (MQ=255)
                                                                   aaCTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTAt                                                                  <  1:431271/140‑1 (MQ=255)
                                                                       ggAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCg                                                              >  1:111286/1‑140 (MQ=255)
                                                                        gAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCgg                                                             <  1:24897/140‑1 (MQ=255)
                                                                        gAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCgg                                                             <  2:246434/140‑1 (MQ=255)
                                                                        gAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCgg                                                             <  2:222336/140‑1 (MQ=255)
                                                                        gAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCgg                                                             <  2:608757/140‑1 (MQ=255)
                                                                        gAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCgg                                                             <  1:741188/140‑1 (MQ=255)
                                                                         agcagcAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGc                                                            <  1:148364/140‑1 (MQ=255)
                                                                         agcagcAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTAATCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGc                                                            <  1:877378/140‑1 (MQ=255)
                                                                                      cgccCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAgg                                               >  1:811686/1‑140 (MQ=255)
                                                                                         ccAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGgcgc                                            <  2:288676/140‑1 (MQ=255)
                                                                                         ccAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGgcgc                                            <  1:58767/140‑1 (MQ=255)
                                                                                                  cAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGaaa                                   <  1:116254/140‑1 (MQ=255)
                                                                                                  cAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGaaa                                   >  2:199997/1‑140 (MQ=255)
                                                                                                  cAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGaaa                                   >  1:607273/1‑140 (MQ=255)
                                                                                                        cgccgcACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTg                             >  1:391673/1‑140 (MQ=255)
                                                                                                         gccgcACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGTCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGc                            <  2:617375/140‑1 (MQ=255)
                                                                                                          ccgcACAACTGGATGCCGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGcc                           <  2:391673/140‑1 (MQ=255)
                                                                                                          ccgcACAACTGGATGCCGCATTCCGTCAGGGAGAGAATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGcc                           >  2:604692/1‑140 (MQ=255)
                                                                                                                          cGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAAcgc           >  2:189601/1‑140 (MQ=255)
                                                                                                                          cGCATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAAcgc           >  2:94845/1‑140 (MQ=255)
                                                                                                                            cATTCCGTCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGt         >  1:578898/1‑140 (MQ=255)
                                                                                                                                   tCAGGGAGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGaagaag  <  2:742767/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                     
CGCGAAACGCAAAACACGCTCAATCAACTCAATAAACAGATTGATGAGATGAACGCGTCGATTGCCAAACTGGAGCAGCAAAAAGCCGCCCAGGAGCGCAGCCTCGCCGCACAACTGGATGCCGCATTCCGTCAGGGCGAGCATACCGGTATTCAGCTGATTCTCAGCGGTGAAGAAAGCCAGCGTGGACAGCGTTTACAGGCTTATTTCGGCTATCTCAACCAGGCGCGACAAGAAACCATTGCCCAGTTGAAGCAAACGCGTGAAGAAG  >  NC_000913/3787084‑3787354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A