Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A4 F73 I1 R1	27	19.6	661800	98.9%	654520	139.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,018,119	G→T	A263S (GCC→TCC)	rmuC →	putative recombination limiting protein RmuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,018,119	0	G	T	100.0%	60.9 / NA	19	A263S (GCC→TCC)	rmuC	putative recombination limiting protein RmuC
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (10/9); total (10/9)

CCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCG  >  NC_000913/4017982‑4018249

ccGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTcc                                                                                                                                  >  2:27415/1‑140 (MQ=255)

        cGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACg                                                                                                                          >  2:213427/1‑140 (MQ=255)

                  atgaatatgaaACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAAc                                                                                                                >  1:121329/1‑140 (MQ=255)

                  atgaatatgaaACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGCGATCGACGCCAAAATGACGGTGGTCTCCTATGAACGCTATTTTAAc                                                                                                                >  1:41621/1‑140 (MQ=255)

                   tgaatatgaaACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACg                                                                                                               <  1:170973/140‑1 (MQ=255)

                              cccAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAgacgac                                                                                                    <  2:121329/140‑1 (MQ=255)

                               ccAGATCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACt                                                                                                   >  2:5583/1‑140 (MQ=255)

                                   gTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTac                                                                                                 >  1:84329/1‑138 (MQ=255)

                                   gTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTac                                                                                                 <  2:84329/138‑1 (MQ=255)

                                              aaaTGACGCCCGCTCGCGGATGCAGCCTGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAgcg                                                                                    <  1:5583/140‑1 (MQ=255)

                                              aaaTGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAgcg                                                                                    <  1:330833/140‑1 (MQ=255)

                                                    cGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTAc                                                                              <  1:27415/140‑1 (MQ=255)

                                                                           aTGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGt                                                       <  1:237526/140‑1 (MQ=255)

                                                                                        ccTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGtt                                          >  2:310028/1‑140 (MQ=255)

                                                                                                            tggtgATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTAt                      >  2:218518/1‑140 (MQ=255)

                                                                                                            tggtgATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTAt                      >  1:290634/1‑140 (MQ=255)

                                                                                                              gtgATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCa                    >  1:36040/1‑140 (MQ=255)

                                                                                                              gtgATCGACGCCAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCa                    >  1:105899/1‑140 (MQ=255)

                                                                                                                         cAAAATGACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCgg         >  1:288815/1‑140 (MQ=255)

                                                                                                                               gACGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGc   <  1:218518/140‑1 (MQ=255)

                                                                                                                                aCGCTGGTCTCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCg  <  2:13934/140‑1 (MQ=255)

CCGGTCTGCGTGAAGGGTATGAATATGAAACCCAGGTCAGCATCGAAAATGACGCCCGCTCGCGGATGCAGCCGGATGTCATCGTGCGCCTGCCGCAGGGAAAAGATGTGGTGATCGACGCCAAAATGACGCTGGTCGCCTATGAACGCTATTTTAACGCCGAAGACGACTACACCCGCGAAAGCGCGCTACAGGAACATATCGCGTCGGTGCGTAACCATATCCGTTTGCTGGGACGCAAAGATTATCAACAGCTGCCGGGGCTGCG  >  NC_000913/4017982‑4018249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A