breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 118,140 C→A C169F (TGC→TTC)  nadC ← quinolinate phosphoribosyltransferase (decarboxylating)
RA 175,221 G→T A39S (GCG→TCG)  clcA → chloride:H(+) antiporter ClcA
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 262,942 C→A noncoding (72/76 nt) thrW → tRNA‑Thr
RA 753,026 T→C intergenic (‑231/+159) ybgD ← / ← gltA putative fimbrial protein YbgD/citrate synthase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,690,971 C→A L374M (CTG→ATG)  ydgA → conserved protein YdgA
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,076,069 A→T I431F (ATC→TTC)  yeeR → CP4‑44 prophage; inner membrane protein YeeR
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,285,185 G→T L270L (CTG→CTT yejM → putative cardiolipin transport protein
RA 2,469,105 A→T K298* (AAA→TAA)  yfdH → CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase
RA 2,515,609 C→A intergenic (+166/+34) insL‑3 → / ← pdeA putative IS186/IS421 transposase/putative c‑di‑GMP phosphodiesterase PdeA
RA 2,830,359 G→T A183S (GCC→TCC)  gutQ → D‑arabinose 5‑phosphate isomerase GutQ
RA 2,834,011 G→T A34S (GCC→TCC)  norW → NADH:flavorubredoxin reductase
RA 3,120,882 G→T A133D (GCT→GAT)  glcA ← glycolate/lactate:H(+) symporter GlcA
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,016,851 G→T V141F (GTT→TTT)  udp → uridine phosphorylase
RA 4,018,119 G→T A263S (GCC→TCC)  rmuC → putative recombination limiting protein RmuC
RA 4,192,613 G→T T8K (ACG→AAG)  thiG ← 1‑deoxy‑D‑xylulose 5‑phosphate:thiol sulfurtransferase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 126323 126373 51 2 [1] [1] 3 aceF pyruvate dehydrogenase, E2 subunit
* * ÷ NC_000913 3103016 3104044 1029 3 [0] [1] 2 mutY adenine DNA glycosylase
* * ÷ NC_000913 3423792–3424234 3424515–3424242 9–724 3 [1] [1] 2 [rrlD] [rrlD]
* * ÷ NC_000913 3603211 3603223 13 2 [1] [0] 2 ftsY signal recognition particle receptor
* * ÷ NC_000913 3619399–3621072 3622174–3621092 21–2776 2 [1] [1] 2 rhsB rhs element protein RhsB
* * ÷ NC_000913 3803440 3803501 62 2 [1] [1] 2 waaB UDP‑D‑galactose:(glucosyl)lipopolysaccharide‑1, 6‑D‑galactosyltransferase

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374922 =NA (NA)7 (0.380) 3/262 2.0 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377329 = NA (NA)intergenic (+18/+206) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 1207790 =18 (0.920)13 (0.750) 13/246 0.3 44.1% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 17 (0.990)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3363001 =31 (1.590)22 (1.140) 20/276 0.1 58.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)30 (1.540) 21/278 0.1 96.8% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 4323260NA (NA)3 (0.170) 3/248 1.9 NA noncoding (21/34 nt) other REP324a
?NC_000913 = 4326069 NA (NA)noncoding (18/34 nt) other REP325f
* ? NC_000913 4373295 =NA (NA)13 (0.720) 6/258 1.2 NA noncoding (2/34 nt) other REP328b
?NC_000913 4374499 = NA (NA)noncoding (1/34 nt) other REP329a