Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A13 F73 I1 R1	31	162.6	5558530	99.2%	5514061	138.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,185,170	G→T	L44L (CTC→CTA)	thiM ←	hydroxyethylthiazole kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,185,170	0	G	T	99.2%	461.2 / ‑3.2	129	L44L (CTC→CTA)	thiM	hydroxyethylthiazole kinase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (50/78); total (50/79)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 129 total aligned reads displayed.

TGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAG  >  NC_000913/2185035‑2185303

tgcttgctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAgcg                                                                                                                                   <  1:1258153/140‑1 (MQ=255)

 gcttgctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAgcgc                                                                                                                                  <  1:773737/140‑1 (MQ=255)

    tgctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAg                                                                                                                               <  1:1735737/140‑1 (MQ=255)

    tgctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAg                                                                                                                               <  1:1531452/140‑1 (MQ=255)

     gctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGc                                                                                                                              <  2:62763/140‑1 (MQ=255)

     gctCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGc                                                                                                                              >  1:1820172/1‑140 (MQ=255)

           cGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTa                                                                                                                        <  2:417064/140‑1 (MQ=255)

               agcaCGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTgg                                                                                                                    <  2:894175/140‑1 (MQ=255)

               agcaCGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTgg                                                                                                                    <  2:2408851/140‑1 (MQ=255)

                 caCGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCg                                                                                                                  >  1:1812171/1‑140 (MQ=255)

                 caCGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCg                                                                                                                  >  2:1081451/1‑140 (MQ=255)

                    gCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTa                                                                                                               <  1:2359532/140‑1 (MQ=255)

                    gCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTa                                                                                                               <  2:2644453/140‑1 (MQ=255)

                    gCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTa                                                                                                               <  1:1135978/140‑1 (MQ=255)

                      aTCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTaaa                                                                                                             >  2:52946/1‑140 (MQ=255)

                      aTCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTaaa                                                                                                             >  1:896268/1‑140 (MQ=255)

                       tCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAg                                                                                                            <  1:471708/140‑1 (MQ=255)

                       tCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAg                                                                                                            <  1:825456/140‑1 (MQ=255)

                            tGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTg                                                                                                       <  2:1678268/140‑1 (MQ=255)

                             gAGCGCGTGGCTGCGTCAGTGTGCCAGCGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGc                                                                                                      <  1:2108194/140‑1 (MQ=255)

                             gAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGc                                                                                                      >  1:202419/1‑140 (MQ=255)

                              aGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTg                                                                                                       >  2:811642/1‑138 (MQ=255)

                              aGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAAGGTTggc                                                                                                     >  2:2650079/1‑138 (MQ=255)

                               gcgcGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGcac                                                                                                    <  2:202419/140‑1 (MQ=255)

                               gcgcGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGcac                                                                                                    <  2:958780/140‑1 (MQ=255)

                                cgcgTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGcacc                                                                                                   >  2:2407214/1‑140 (MQ=255)

                                 gcgTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGcacca                                                                                                  <  2:378415/140‑1 (MQ=255)

                                 gcgTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCAATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGcacca                                                                                                  <  2:2052224/140‑1 (MQ=255)

                                  cgTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCTCCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGcaccac                                                                                                 <  2:2664045/140‑1 (MQ=255)

                                  cgTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGcaccac                                                                                                 >  1:1293600/1‑140 (MQ=255)

                                    tGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTCAAGGGTTTgaccaccc                                                                                               >  2:2304384/1‑138 (MQ=255)

                                    tGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGcaccac                                                                                                 <  2:950595/138‑1 (MQ=255)

                                    tGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGcaccac                                                                                                 >  1:950595/1‑138 (MQ=255)

                                    tGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACcacac                                                                                               >  2:1596318/1‑139 (MQ=255)

                                     ggCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCAcatc                                                                                              <  1:1954484/140‑1 (MQ=255)

                                       cTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCAcatcat                                                                                            <  2:2119947/140‑1 (MQ=255)

                                       cTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCAcatcat                                                                                            <  1:2426329/140‑1 (MQ=255)

                                       cTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCAcatcat                                                                                            <  2:1483856/140‑1 (MQ=255)

                                       cTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCAcatcat                                                                                            <  1:884361/140‑1 (MQ=255)

                                       cTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCAcatcat                                                                                            <  1:1094441/140‑1 (MQ=255)

                                       cTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCAcatcat                                                                                            <  1:2449242/140‑1 (MQ=255)

                                       cTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCAcatcat                                                                                            <  1:40830/140‑1 (MQ=255)

                                          cGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTgg                                                                                         <  1:52946/140‑1 (MQ=255)

                                               gtgtGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATg                                                                                    <  2:162308/140‑1 (MQ=255)

                                               gtgtGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATg                                                                                    <  1:2309078/140‑1 (MQ=255)

                                               gtgtGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATg                                                                                    <  1:2121147/140‑1 (MQ=255)

                                                 gtgCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCa                                                                                  <  2:1744988/140‑1 (MQ=255)

                                                 gtgCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCa                                                                                  <  2:1216778/140‑1 (MQ=255)

                                                  tgCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAg                                                                                 >  1:1846252/1‑140 (MQ=255)

                                                    ccAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTg                                                                               >  1:175999/1‑140 (MQ=255)

                                                    ccAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTg                                                                               <  1:271610/140‑1 (MQ=255)

                                                     cAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTTTACCCCATCATTGGTCATGAAGTGc                                                                              >  2:2511027/1‑140 (MQ=255)

                                                      aaCGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGca                                                                             <  1:978723/140‑1 (MQ=255)

                                                      aaCGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGca                                                                             <  1:518835/140‑1 (MQ=255)

                                                       aCGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCACATCATTGGTCATGCAGTGcac                                                                            >  2:1879704/1‑140 (MQ=255)

                                                         gTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACaa                                                                          <  2:669419/140‑1 (MQ=255)

                                                         gTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACaa                                                                          <  2:175999/140‑1 (MQ=255)

                                                         gTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACaa                                                                          <  1:1426896/140‑1 (MQ=255)

                                                            aaTCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAagag                                                                       >  1:1778108/1‑140 (MQ=255)

                                                            aaTCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTTCACCACATCATTTGTCATGCAGTGGACAagag                                                                       >  2:1939613/1‑140 (MQ=255)

                                                              tCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAggg                                                                     <  1:1081451/140‑1 (MQ=255)

                                                                  caaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAAt                                                                 >  1:2633907/1‑140 (MQ=255)

                                                                   aaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGca                                                                             <  1:2320650/127‑1 (MQ=255)

                                                                   aaGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGca                                                                             >  2:2320650/1‑127 (MQ=255)

                                                                    aGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGGATTGGCGGGAAAAGTTTGCACCCCACCATTGGTACTGCCCAGCACAAGAGGGGAATGt                                                               >  2:111009/1‑140 (MQ=255)

                                                                    aGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGAATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACaa                                                                          >  1:2021273/1‑129 (MQ=255)

                                                                    aGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGAATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACaa                                                                          <  2:2021273/129‑1 (MQ=255)

                                                                     ggCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGtt                                                              <  2:51480/140‑1 (MQ=255)

                                                                     ggCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGGCATGCAGTGCACAAGAGGGGGATGtt                                                              >  1:2189925/1‑140 (MQ=255)

                                                                        aCTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGt                                                           >  2:1440723/1‑140 (MQ=255)

                                                                          tGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGa                                                         <  2:1389164/140‑1 (MQ=255)

                                                                          tGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGa                                                         >  1:26307/1‑140 (MQ=255)

                                                                            gCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGaaa                                                       <  1:2139199/140‑1 (MQ=255)

                                                                            gCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGaaa                                                       <  1:2182872/140‑1 (MQ=255)

                                                                            gCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGaaa                                                       <  2:2693090/140‑1 (MQ=255)

                                                                            gCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCCCATCATTTGTCATGCAGTGCACAAGAGGGGGAAGTTGGTGgaa                                                       >  2:977955/1‑140 (MQ=255)

                                                                            gCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAAGGTTGCACCACAACATTTGTCATGCAGGGCACAAGAGGGGGAAGTTGGTGaaa                                                       >  2:1450507/1‑140 (MQ=255)

                                                                                tAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAgg                                                   >  2:1818466/1‑140 (MQ=255)

                                                                                      cAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTTGTCATGCAGTGCACAAGAGGGGGATGTTGGTGGAAAAAGGGTaaa                                             >  2:1613135/1‑139 (MQ=255)

                                                                                      cAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGGATGTTGGTGAAAAAGGGGTaaa                                             >  1:2535651/1‑139 (MQ=255)

                                                                                      cAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGt                                                           >  1:1239975/1‑126 (MQ=255)

                                                                                      cAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGt                                                           <  2:1239975/126‑1 (MQ=255)

                                                                                      cAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGaaa                                                       >  1:796342/1‑130 (MQ=255)

                                                                                      cAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGaaa                                                       <  2:796342/130‑1 (MQ=255)

                                                                                      cAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAAc                                             >  1:1588434/1‑140 (MQ=255)

                                                                                      cAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAAc                                             >  1:1066553/1‑140 (MQ=255)

                                                                                       aaaCTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAAcg                                            <  2:2633907/140‑1 (MQ=255)

                                                                                                     ctTCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTTGGCATGCAGGGCACAAGAGGGGGATGTTGGTGAAAAAAGGGTAACGCGTGCGCAGATTgg                              >  2:2703222/1‑139 (MQ=255)

                                                                                                      ttCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTgcg                             <  1:740533/140‑1 (MQ=255)

                                                                                                      ttCGGTTTCGATAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTgcg                             <  1:2703222/140‑1 (MQ=255)

                                                                                                                aTAACCATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAAGGTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCcag                   >  2:2302831/1‑140 (MQ=255)

                                                                                                                    ccATCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGATTGGTCCACATCATTTGTCATGCAGTGCACAAGAGGGGGATGTTGGTGAAAAAGGGGGAACGCGTGCGCCGATTGCGCTGGACCCAGCAgg               >  2:2465344/1‑140 (MQ=255)

                                                                                                                      aTCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTc             <  2:2724727/140‑1 (MQ=255)

                                                                                                                      aTCGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTc             <  2:342396/140‑1 (MQ=255)

                                                                                                                        cGCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGGTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGGATGTTGGGGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGGCGa           >  1:1767216/1‑140 (MQ=255)

                                                                                                                         gCTGGCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGAc          <  1:2407214/140‑1 (MQ=255)

                                                                                                                            ggCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTg       <  1:1879704/140‑1 (MQ=255)

                                                                                                                             gCGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGc      <  1:2662644/140‑1 (MQ=255)

                                                                                                                              cGATGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCa     <  2:1778108/140‑1 (MQ=255)

                                                                                                                                 tGCACCTAGCGCCAGCAAGGTATTGGCGGTAAAAGGTTGCACCACATCATTTGGCATGCAGTGCACAAGAGGGGGATGTTGGGGAAAAAAGGGTAACGCGGGCGCAGATTGCGCTGAACCCCGCAGGGCGACTTGCATAg  >  2:1327253/1‑140 (MQ=255)

TGCTTGCTCAACGGCAGCACGCATCGCCTGAGCGCGTGGCTGCGTCAGTGTGCCAACGTTAATCAACAAGGCACTGGCGATAGCCGCAAACTGACTGGCCTCTTCGGTTTCGATAACCATCGCTGGCGATGCACCGAGCGCCAGCAAGGTATTGGCGGTAAAGGTTTGCACCACATCATTGGTCATGCAGTGCACAAGAGGGGAATGTTGGTGAAAAAGGTGTAACGCGTGCGCAGATTGCGCTGAACCCAGCAGGTCGACTTGCATAG  >  NC_000913/2185035‑2185303

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A