breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 232,873 G→T S361R (AGC→AGA mltD ← membrane‑bound lytic murein transglycosylase D
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 641,714 C→A A52S (GCC→TCC)  uspG ← universal stress protein G
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 987,174 (A)6→5 intergenic (‑192/+411) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 994,645 G→T A63E (GCG→GAG)  ssuC ← aliphatic sulfonate ABC transporter membrane subunit
RA 1,197,105 G→T S143* (TCA→TAA)  ymfD ← e14 prophage; putative SAM‑dependent methyltransferase
RA 1,541,844 G→A Y249Y (TAC→TAT narZ ← nitrate reductase Z subunit alpha
RA 1,557,074 C→A intergenic (+18/+38) osmC → / ← ddpF osmotically inducible peroxiredoxin/putative D,D‑dipeptide ABC transporter ATP‑binding subunit DdpF
RA 1,576,247 C→A R458L (CGC→CTC)  yddB ← putative TonB‑dependent receptor
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,699,247 C→A intergenic (‑67/‑108) malI ← / → malX DNA‑binding transcriptional repressor MalI/PTS enzyme IIBC component MalX
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,185,170 G→T L44L (CTC→CTA thiM ← hydroxyethylthiazole kinase
RA 2,192,725 C→A E24D (GAG→GAT yehE ← DUF2574 domain‑containing protein YehE
RA 2,417,593 C→A A171A (GCC→GCA yfcC → putative transporter YfcC
RA 2,457,501 G→T A553A (GCC→GCA fadJ ← 3‑hydroxyacyl‑CoA dehydrogenase FadJ
RA 3,193,440 G→T E193* (GAA→TAA)  yqiK → protein YqiK
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,561,162 G→T T97T (ACC→ACA glpG ← rhomboid protease
RA 3,761,653 C→A M101I (ATG→ATT yibF ← glutathione transferase‑like protein YibF
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,030,712 C→A W87L (TGG→TTG)  fadB ← dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase
RA 4,200,061 T→C G186G (GGT→GGC yjaG → conserved protein YjaG
RA 4,253,014 (A)7→6 intergenic (+11/‑2) ubiC → / → ubiA chorismate lyase/4‑hydroxybenzoate octaprenyltransferase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,424,472 G→T intergenic (+86/+44) ulaF → / ← yjfY L‑ribulose‑5‑phosphate 4‑epimerase UlaF/DUF1471 domain‑containing protein YjfY

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102970 3104061 1092 46 [45] [45] 46 [mutY] [mutY]
* * ÷ NC_000913 3423752–3424234 3424527–3424238 5–776 46 [45] [45] 46 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =49 (0.300)203 (1.400) 92/244 0.1 84.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 33 (0.230)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780549 (0.300)131 (0.910) 71/244 0.3 77.5% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 33 (0.230)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)247 (1.550) 107/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =235 (1.440)256 (1.580) 98/274 0.1 68.5% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.010)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)173 (1.060) 80/276 0.3 98.9% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.010)coding (3907/4134 nt) rhsA rhs element protein RhsA