Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F73 I1 R1 31 162.6 5558530 99.2% 5514061 138.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,030,712 C→A W87L (TGG→TTG)  fadB ← dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,030,7120CA100.0% 460.7 / NA 131W87L (TGG→TTG) fadBdodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (63/68);  total (63/68)

Only 100 of 136 total aligned reads displayed.

GATCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGC  >  NC_000913/4030575‑4030848
                                                                                                                                         |                                                                                                                                        
gATCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAAc                                                                                                                                        <  2:110277/140‑1 (MQ=255)
 aTCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACt                                                                                                                                       >  1:1258248/1‑140 (MQ=255)
 aTCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACt                                                                                                                                       <  2:2376335/140‑1 (MQ=255)
   ccGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGa                                                                                                                                     <  1:336104/140‑1 (MQ=255)
        gTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACTCTATTGGCAAAGTGCAGCAACTGACTTaa                                                                                                                                <  1:2360341/140‑1 (MQ=255)
        gTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTaa                                                                                                                                <  2:861836/140‑1 (MQ=255)
        gTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTaa                                                                                                                                <  1:737599/140‑1 (MQ=255)
           gCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGa                                                                                                                                     <  1:106218/132‑1 (MQ=255)
           gCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTg                                                                                                                             >  2:2208521/1‑140 (MQ=255)
           gCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTg                                                                                                                             >  1:2770174/1‑140 (MQ=255)
           gCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTg                                                                                                                             >  2:1395233/1‑140 (MQ=255)
           gCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTg                                                                                                                             <  2:1858966/140‑1 (MQ=255)
           gCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTg                                                                                                                             <  2:2026727/140‑1 (MQ=255)
           gCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTg                                                                                                                             <  2:2752986/140‑1 (MQ=255)
                   aTAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAg                                                                                                                     <  2:2361213/140‑1 (MQ=255)
                    tAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAgg                                                                                                                    <  1:2581861/140‑1 (MQ=255)
                    tAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAgg                                                                                                                    <  1:1036947/140‑1 (MQ=255)
                     aaTCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGa                                                                                                                   >  2:2204895/1‑140 (MQ=255)
                     aaTCGGTCGACAGCACGCATTCGCAGCCACCGACCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAAGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGa                                                                                                                   <  2:707093/140‑1 (MQ=255)
                      aTCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGttcttc                                                                                                                       >  1:2039073/1‑135 (MQ=255)
                      aTCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGaa                                                                                                                  <  1:1828255/140‑1 (MQ=255)
                        cGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTAACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACg                                                                                                                <  2:820041/140‑1 (MQ=255)
                          gTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTg                                                                                                                             >  1:489679/1‑125 (MQ=255)
                          gTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTg                                                                                                                             <  2:489679/125‑1 (MQ=255)
                          gTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAgg                                                                                                                    >  1:542457/1‑134 (MQ=255)
                          gTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAgg                                                                                                                    <  2:542457/134‑1 (MQ=255)
                          gTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAg                                                                                                              >  2:2382068/1‑140 (MQ=255)
                           tCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGa                                                                                                                   >  2:1795532/1‑134 (MQ=255)
                           tCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGa                                                                                                                   <  1:1795532/134‑1 (MQ=255)
                              ccAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGa                                                                                                                   <  1:1770298/131‑1 (MQ=255)
                                  cACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAggg                                                                                                      >  2:619947/1‑140 (MQ=255)
                                  cACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAggg                                                                                                      >  2:209366/1‑140 (MQ=255)
                                  cACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAggg                                                                                                      >  1:267653/1‑140 (MQ=255)
                                   aCGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGa                                                                                                     <  1:424618/140‑1 (MQ=255)
                                     gCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACa                                                                                                   >  1:1491008/1‑140 (MQ=255)
                                        ttCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACaaaa                                                                                                <  2:269761/140‑1 (MQ=255)
                                          cGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAAt                                                                                              <  1:386173/140‑1 (MQ=255)
                                            cAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAAt                                                                                              >  2:938139/1‑138 (MQ=255)
                                                cACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTg                                                                                        <  1:825839/140‑1 (MQ=255)
                                                 aCCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGa                                                                                       >  2:1507207/1‑140 (MQ=255)
                                                 aCCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGa                                                                                       >  1:155504/1‑140 (MQ=255)
                                                   cGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAGCGAGGAACAGGGACAAAAATTCGGTGata                                                                                     >  1:2376865/1‑140 (MQ=255)
                                                   cGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGata                                                                                     >  1:2137557/1‑140 (MQ=255)
                                                     cccAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATc                                                                                   >  1:1117124/1‑140 (MQ=255)
                                                        aGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGc                                                                                <  2:486172/140‑1 (MQ=255)
                                                         gcgcATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCa                                                                               >  2:2355636/1‑140 (MQ=255)
                                                         gcgcATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCa                                                                               >  1:594763/1‑140 (MQ=255)
                                                          cgcATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCAc                                                                              <  2:1179448/140‑1 (MQ=255)
                                                             aTAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCa                                                                               >  1:2143673/1‑136 (MQ=255)
                                                                 ccATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGAt                                                                       <  1:2611728/140‑1 (MQ=255)
                                                                    ttGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATaaa                                                                    >  2:1770741/1‑140 (MQ=255)
                                                                    ttGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATaaa                                                                    >  1:1499887/1‑140 (MQ=255)
                                                                     tGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAg                                                                   >  1:2362478/1‑140 (MQ=255)
                                                                     tGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAg                                                                   >  2:740097/1‑140 (MQ=255)
                                                                           cagcaATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAGGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCAc                                                                              >  1:482590/1‑123 (MQ=255)
                                                                           cagcaATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGgctgct                                                             >  1:1492588/1‑140 (MQ=255)
                                                                           cagcaATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGgctgct                                                             <  2:923451/140‑1 (MQ=255)
                                                                           cagcaATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGgctgct                                                             >  1:767504/1‑140 (MQ=255)
                                                                           cagcaATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGgctgct                                                             >  2:1774695/1‑140 (MQ=255)
                                                                              caATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTg                                                          >  2:1749812/1‑140 (MQ=255)
                                                                                    tCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGaa                                                    <  2:2105657/140‑1 (MQ=255)
                                                                                       gCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACgc                                                 <  1:1194484/140‑1 (MQ=255)
                                                                                        cACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAgg                                                                  >  1:967226/1‑122 (MQ=255)
                                                                                             gCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACgcagcagc                                           >  1:1303394/1‑140 (MQ=255)
                                                                                             gCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACgc                                                 >  2:812269/1‑134 (MQ=255)
                                                                                              cAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACgcagcagca                                          <  1:861912/140‑1 (MQ=255)
                                                                                               aGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGcagcagcag                                         <  2:2362478/140‑1 (MQ=255)
                                                                                               aGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGcagcagcag                                         <  1:1395233/140‑1 (MQ=255)
                                                                                               aGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGcagcagcag                                         <  1:2208521/140‑1 (MQ=255)
                                                                                                gATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAGCAGGGACAAAAAGTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCagcagcagc                                        <  2:1289946/140‑1 (MQ=255)
                                                                                                gATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCagcagcagc                                        <  1:452391/140‑1 (MQ=255)
                                                                                                gATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCagcagcagc                                        <  1:782715/140‑1 (MQ=255)
                                                                                                gATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCagcagcagc                                        <  1:740097/140‑1 (MQ=255)
                                                                                                 aTCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGcc                                       <  1:2382068/140‑1 (MQ=255)
                                                                                                 aTCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGcc                                       <  2:2268738/140‑1 (MQ=255)
                                                                                                   cTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCt                                     >  1:941625/1‑140 (MQ=255)
                                                                                                      ccAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCagcagcagc                                        >  1:2387131/1‑134 (MQ=255)
                                                                                                      ccAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCagcagcagc                                        <  2:2387131/134‑1 (MQ=255)
                                                                                                       cAGGCGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTa                                 >  1:1886311/1‑140 (MQ=255)
                                                                                                           cGATTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATc                             >  1:456842/1‑140 (MQ=255)
                                                                                                             aTTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTg                           >  2:1548620/1‑140 (MQ=255)
                                                                                                             aTTAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTg                           <  1:1770741/140‑1 (MQ=255)
                                                                                                              ttAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCtt                                    >  1:1744743/1‑130 (MQ=255)
                                                                                                              ttAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCtt                                    <  2:1744743/130‑1 (MQ=255)
                                                                                                              ttAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGa                          >  2:1460478/1‑140 (MQ=255)
                                                                                                              ttAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGa                          >  2:350770/1‑140 (MQ=255)
                                                                                                              ttAAACACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGa                          >  1:308838/1‑140 (MQ=255)
                                                                                                                 aaCACGCTATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTg                       <  2:264524/140‑1 (MQ=255)
                                                                                                                        tATTGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTcc                >  1:2361771/1‑140 (MQ=255)
                                                                                                                          ttGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAg              >  1:668610/1‑140 (MQ=255)
                                                                                                                           tGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGc             >  1:218628/1‑140 (MQ=255)
                                                                                                                           tGGCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGc             >  1:1326373/1‑140 (MQ=255)
                                                                                                                            ggCAAAGTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCa            <  1:1760109/140‑1 (MQ=255)
                                                                                                                                  gTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGa      >  1:972328/1‑140 (MQ=255)
                                                                                                                                  gTGCAGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGa      >  2:1697812/1‑140 (MQ=255)
                                                                                                                                    gcagcaACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATg    >  1:1468673/1‑140 (MQ=255)
                                                                                                                                    gcagcaACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATg    >  1:902076/1‑140 (MQ=255)
                                                                                                                                      aGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGc  <  2:456842/140‑1 (MQ=255)
                                                                                                                                      aGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGc  <  2:1117124/140‑1 (MQ=255)
                                                                                                                                      aGCAACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGc  <  2:2006801/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
GATCCGGCGTCGCCAGACGATAATCGGTCGCCAGCACGCATTCGCAGCCACCGCCCAGCGCATAGCCATTGACGGCAGCAATGGTCGGCACCGGCAGATCTTCCAGGCGATTAAACACGCTATTGGCAAAGTGCAGCCACTGACTTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGC  >  NC_000913/4030575‑4030848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A