Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A5 F73 I1 R1 30 41.4 1413620 99.2% 1402311 139.5

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,071,172 C→A pseudogene (387/426 nt) yeeP → CP4‑44 prophage; putative GTP‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,071,1720CA100.0% 159.0 / NA 46pseudogene (387/426 nt)yeePCP4‑44 prophage; putative GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (21/25);  total (21/25)

GAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAG  >  NC_000913/2071044‑2071297
                                                                                                                                |                                                                                                                             
gAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGCATCTGTTGCCCg                                                                                                                    >  2:129573/1‑140 (MQ=255)
  aTCTGTCCGGTTTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGGCCGGATATTTGACGCGGCGGAGGGCGTCTGTATTGCCTTTGTTGCGCGCACGGTTCTGCGTGCCGTCTGTGACACGGTGGTATCTGTTGCCCGTg                                                                                                                  <  2:285282/140‑1 (MQ=255)
  aTCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTg                                                                                                                  <  2:140241/140‑1 (MQ=255)
  aTCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTg                                                                                                                  <  2:86633/140‑1 (MQ=255)
    cTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCg                                                                                                                <  2:331518/140‑1 (MQ=255)
           gTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGa                                                                                                         >  1:251985/1‑140 (MQ=255)
                   cccGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGAtt                                                                                                 <  2:318775/140‑1 (MQ=255)
                              tttACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAAc                                                                                      >  2:93093/1‑140 (MQ=255)
                              tttACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAAc                                                                                      <  1:474429/140‑1 (MQ=255)
                              tttACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAAc                                                                                      <  1:284355/140‑1 (MQ=255)
                                     gtgcggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGt                                                                               <  2:23267/140‑1 (MQ=255)
                                      tgcggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTg                                                                              >  2:89704/1‑140 (MQ=255)
                                        cggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGAc                                                                            >  2:615877/1‑140 (MQ=255)
                                        cggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGAc                                                                            >  2:226984/1‑140 (MQ=255)
                                        cggtgGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGGATGGAACTGGATTTTCTTCTGAACCTGTCGTGAc                                                                            >  1:123157/1‑140 (MQ=255)
                                            ggACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGAt                                                                        <  1:318627/140‑1 (MQ=255)
                                                  gATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGccctc                                                                  >  1:560493/1‑140 (MQ=255)
                                                  gATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGccctc                                                                  >  1:252046/1‑140 (MQ=255)
                                                    tatTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGccctccc                                                                <  1:679567/140‑1 (MQ=255)
                                                     atTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGccctccct                                                               <  2:567733/140‑1 (MQ=255)
                                                     atTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGccctccct                                                               <  2:87689/140‑1 (MQ=255)
                                                               gcggAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGt                                                     <  2:251985/140‑1 (MQ=255)
                                                                    gagCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCt                                                <  1:447539/140‑1 (MQ=255)
                                                                        gTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTcaca                                            >  2:514384/1‑140 (MQ=255)
                                                                         tCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACaa                                           >  2:224266/1‑140 (MQ=255)
                                                                          cTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACaaa                                          >  1:350246/1‑140 (MQ=255)
                                                                            gTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAg                                        <  2:560493/140‑1 (MQ=255)
                                                                              aTTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCa                                      <  2:421494/140‑1 (MQ=255)
                                                                                       gTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCg                             >  1:613308/1‑140 (MQ=255)
                                                                                       gTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCg                             <  1:226984/140‑1 (MQ=255)
                                                                                       gTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCg                             >  2:624526/1‑140 (MQ=255)
                                                                                         tGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGtt                           <  1:423848/140‑1 (MQ=255)
                                                                                             cgcacgGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTg                       <  1:583261/140‑1 (MQ=255)
                                                                                             cgcacgGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTg                       <  2:548040/140‑1 (MQ=255)
                                                                                              gcacgGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGt                      <  1:430751/140‑1 (MQ=255)
                                                                                                acgGTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGtct                    >  2:180295/1‑140 (MQ=255)
                                                                                                  ggTTCTGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGtctct                  <  1:224266/140‑1 (MQ=255)
                                                                                                       tGCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGt             <  2:435128/140‑1 (MQ=255)
                                                                                                        gCGTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTc            >  2:80485/1‑140 (MQ=255)
                                                                                                          gTGCCGTCCGTGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCg          <  1:514384/140‑1 (MQ=255)
                                                                                                              cgtccgtGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCa      >  1:93343/1‑140 (MQ=255)
                                                                                                              cgtccgtGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCa      >  2:631203/1‑140 (MQ=255)
                                                                                                              cgtccgtGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCa      >  2:320988/1‑140 (MQ=255)
                                                                                                                tccgtGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAAt    >  2:656809/1‑140 (MQ=255)
                                                                                                                tccgtGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAAt    >  2:436369/1‑140 (MQ=255)
                                                                                                                tccgtGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAAt    <  1:129573/140‑1 (MQ=255)
                                                                                                                  cgtGACACGGTGGTATCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAg  <  1:615877/140‑1 (MQ=255)
                                                                                                                                |                                                                                                                             
GAATCTGTCCGGTCTCAGGCCCGTGAACAGTTTACCGGTGCGGTGGACCGGATATTTGACACGGCGGAGAGCGTCTGTATTGCCTCTGTTGCACGCACGGTTCTGCGTGCCGTCCGTGACACGGTGGTCTCTGTTGCCCGTGCGGTATGGAACTGGATTTTCTTCTGAACCTGTCGTGACTGATGCCCTCCCTGACTCTGAGTCTGCTCACAAAAGCACTGTTTTCGTTACTGTCTCTCTTGTCCGTGCAATAG  >  NC_000913/2071044‑2071297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A