breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 178,175 C→A Q96H (CAG→CAT btuF ← vitamin B12 ABC transporter periplasmic binding protein
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 877,751 C→A P95P (CCG→CCT rimO ← ribosomal protein S12 methylthiotransferase RimO
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,049,923 C→A L31I (CTC→ATC)  insA‑4 → IS1 protein InsA
RA 1,287,240 G→T intergenic (‑4/+4) rttR ← / ← tyrV small RNA RttR/tRNA‑Tyr
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,741,589 C→A G59G (GGC→GGA cfa → cyclopropane fatty acyl phospholipid synthase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,071,172 C→A pseudogene (387/426 nt) yeeP → CP4‑44 prophage; putative GTP‑binding protein
RA 2,091,937 G→A V281I (GTT→ATT)  hisD → histidinal/histidinol dehydrogenase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,274,149 C→A L596M (CTG→ATG)  yejA → putative oligopeptide ABC transporter periplasmic binding protein
RA 2,483,187 C→A T51T (ACG→ACT emrK ← tripartite efflux pump membrane fusion protein EmrK
RA 2,522,486 G→T intergenic (‑9/+243) xapR ← / ← xapB DNA‑binding transcriptional activator XapR/xanthosine:H(+) symporter XapB
RA 2,946,395 C→A A262S (GCT→TCT)  mltA ← membrane‑bound lytic murein transglycosylase A
RA 3,023,896 G→T G861C (GGC→TGC)  xdhD → fused putative xanthine/hypoxanthine oxidase: molybdopterin‑binding subunit and Fe‑S binding subunit
RA 3,091,169 G→T L12L (CTG→CTT rsmE → 16S rRNA m(3)U1498 methyltransferase
RA 3,340,494 C→A L74I (CTC→ATC)  yrbG → putative transport protein YrbG
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,689,231 T→G D344A (GAT→GCT)  bcsZ ← endo‑1,4‑D‑glucanase
RA 3,750,633 C→A A183D (GCC→GAC)  sgbE → L‑ribulose‑5‑phosphate 4‑epimerase SgbE
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,824,561 G→A R16Q (CGG→CAG)  trmH → tRNA (Gm18) 2'‑O‑methyltransferase
RA 4,028,608 C→A W55C (TGG→TGT fadA ← 3‑ketoacyl‑CoA thiolase
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,572,231 G→T intergenic (+316/‑183) yjiS → / → yjiT DUF1127 domain‑containing protein YjiS/putative uncharacterized protein YjiT

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102970 3104091 1122 9 [8] [8] 10 mutY mutY
* * ÷ NC_000913 3423791–3424233 3424551–3424242 10–761 9 [7] [8] 9 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374922 =NA (NA)20 (0.510) 11/262 1.9 NA intergenic (+41/‑537) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377329 = NA (NA)intergenic (+18/+206) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 1207790 =33 (0.790)12 (0.330) 12/246 1.6 26.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 39 (1.060)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780533 (0.790)9 (0.240) 7/246 2.6 20.9% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 39 (1.060)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)66 (1.630) 48/270 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =73 (1.750)58 (1.400) 40/276 0.1 61.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 0 (0.000)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)61 (1.460) 40/278 0.1 95.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA