Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A5 F73 I1 R1	30	41.4	1413620	99.2%	1402311	139.5

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,824,561	G→A	R16Q (CGG→CAG)	trmH →	tRNA (Gm18) 2'‑O‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,824,561	0	G	A	100.0%	134.9 / NA	39	R16Q (CGG→CAG)	trmH	tRNA (Gm18) 2'‑O‑methyltransferase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (17/22); total (17/22)

GACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACC  >  NC_000913/3824428‑3824691

gACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGcc                                                                                                                              <  2:689646/140‑1 (MQ=255)

       tgCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTg                                                                                                                       <  1:573987/140‑1 (MQ=255)

            cTGGCGAATATCATGCGCAAAATCTGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGt                                                                                                                  <  1:265104/140‑1 (MQ=255)

            cTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGt                                                                                                                  <  1:627224/140‑1 (MQ=255)

             tGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTc                                                                                                                 >  1:57632/1‑140 (MQ=255)

                 gAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCa                                                                                                             <  1:215312/140‑1 (MQ=255)

                 gAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCa                                                                                                             <  2:162098/140‑1 (MQ=255)

                         tGCGCAAAATCCGCGTGATGCCAGACGTGATTAGAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAg                                                                                                     >  1:260560/1‑140 (MQ=255)

                         tGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGt                                                                                                                  <  2:699916/127‑1 (MQ=255)

                         tGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGt                                                                                                                  >  1:699916/1‑127 (MQ=255)

                         tGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAg                                                                                                     >  1:124833/1‑140 (MQ=255)

                           cgcAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAg                                                                                                        <  2:421302/135‑1 (MQ=255)

                           cgcAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAg                                                                                                        >  1:421302/1‑135 (MQ=255)

                            gcAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTc                                                                                                  >  1:138906/1‑140 (MQ=255)

                                 aTCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACaa                                                                                             <  1:577153/140‑1 (MQ=255)

                                     gcgTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCt                                                                                         <  1:81172/140‑1 (MQ=255)

                                     gcgTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCt                                                                                         <  1:246511/140‑1 (MQ=255)

                                            gCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACg                                                                                  >  1:128553/1‑140 (MQ=255)

                                                    tGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCg                                                                          <  2:124833/140‑1 (MQ=255)

                                                         aaaGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGattat                                                                     <  2:160177/140‑1 (MQ=255)

                                                               aCCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTAc                                                               >  2:691448/1‑140 (MQ=255)

                                                               aCCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTAc                                                               >  1:92085/1‑140 (MQ=255)

                                                               aCCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTAc                                                               >  1:317462/1‑140 (MQ=255)

                                                                 ccgaaaccgaaaTTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCg                                                             <  2:493723/140‑1 (MQ=255)

                                                                   gaaaccgaaaTTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCATAAACCTCATAACGTTTCTGCGATTATTCGTACCGCa                                                           <  1:61804/140‑1 (MQ=255)

                                                                   gaaaccgaaaTTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCa                                                           <  2:186268/140‑1 (MQ=255)

                                                                     aaccgaaaTTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGa                                                         <  2:57632/140‑1 (MQ=255)

                                                                               aaTGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGc                                               <  1:319207/140‑1 (MQ=255)

                                                                                 tGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGt                                             <  2:317462/140‑1 (MQ=255)

                                                                                      tATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATg                                        >  1:296203/1‑140 (MQ=255)

                                                                                        tGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGaa                                      >  1:294240/1‑140 (MQ=255)

                                                                                                      tATGCACGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGGACATGAATTTCACGCCGTCTg                        >  2:500904/1‑140 (MQ=255)

                                                                                                           aCGCATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTg                   <  1:691448/140‑1 (MQ=255)

                                                                                                              cATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTa                >  1:263455/1‑140 (MQ=255)

                                                                                                              cATCTGCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTa                >  1:504669/1‑140 (MQ=255)

                                                                                                                   gCGAAATGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGc           <  2:379094/140‑1 (MQ=255)

                                                                                                                        aTGCTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCTGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATgcg      <  1:154233/140‑1 (MQ=255)

                                                                                                                           cTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCAc   >  2:244787/1‑140 (MQ=255)

                                                                                                                           cTCGCCAGGCAGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCAc   >  1:641364/1‑140 (MQ=255)

                                                                                                                            tCGCCAGGTAGCAGCCTGATCTGTCCGTTGGGATGGAGCGAGTCCGCAAACCTGATATCGTTTCTGCGATTATTCGTACCGCAGATGCGGTTGGCGTACATGAAGTTCTCGCCGGCTGGCCTGTTAGCCGCATGCGCGcc  <  2:100202/140‑1 (MQ=255)

GACCGTGTGCATCTGGCGAATATCATGCGCAAAATCCGCGTGATGCCAGACGTGATTAAAGTCACCCGAAACCGAAATTAATGTTTTATGAACCCAACACGTTATGCACGCATCTGCGAAATGCTCGCCAGGCGGCAGCCTGATCTGACCGTCTGCATGGAGCAGGTCCACAAACCTCATAACGTTTCTGCGATTATTCGTACCGCAGATGCCGTTGGCGTACATGAAGTTCACGCCGTCTGGCCTGGTAGCCGCATGCGCACC  >  NC_000913/3824428‑3824691

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A