Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F73 I1 R1	31	34.8	1203930	99.1%	1193094	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,156,198	G→A	M312I (ATG→ATA)	mdtB →	multidrug efflux pump RND permease subunit MdtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,156,198	0	G	A	100.0%	129.5 / NA	38	M312I (ATG→ATA)	mdtB	multidrug efflux pump RND permease subunit MdtB
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (16/22); total (16/22)

TCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCAT  >  NC_000913/2156065‑2156329

ccGCAACGGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGGGGGCGAACAAAGAACAGGCCATTGTGATGAATGTCCAGCGCCAGCCCGGTGCTAACATTATCGCCACCGCCGACAGCATTCGGCAGATACTGcct                                                                                                                               <  2:510893/139‑2 (MQ=255)

     aCTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCt                                                                                                                          <  1:256775/140‑1 (MQ=255)

        gTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCAc                                                                                                                       <  1:232659/140‑1 (MQ=255)

          agagCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTg                                                                                                                     <  1:542609/140‑1 (MQ=255)

           gagCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTga                                                                                                                    >  1:358766/1‑140 (MQ=255)

              cAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGagag                                                                                                                 >  2:287141/1‑140 (MQ=255)

                   tGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAg                                                                                                                            <  2:492355/124‑1 (MQ=255)

                   tGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAg                                                                                                                            >  1:492355/1‑124 (MQ=255)

                      aGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGa                                                                                                         >  2:464233/1‑140 (MQ=255)

                           aCAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCg                                                                                                    <  2:53984/140‑1 (MQ=255)

                             aGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGt                                                                                                  >  1:176099/1‑140 (MQ=255)

                               cTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGa                                                                                                >  1:406900/1‑140 (MQ=255)

                                tGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGaa                                                                                               <  1:252941/140‑1 (MQ=255)

                                tGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGaa                                                                                               <  2:407113/140‑1 (MQ=255)

                                     cGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGa                                                                                          <  2:374226/140‑1 (MQ=255)

                                                       aGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGca                                                                        <  1:473272/140‑1 (MQ=255)

                                                         aaCAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGcacc                                                                      >  2:217749/1‑140 (MQ=255)

                                                              gCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCaa                                                                 >  1:206097/1‑140 (MQ=255)

                                                                   tgtgATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATcc                                                            >  1:193624/1‑140 (MQ=255)

                                                                   tgtgATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATcc                                                            >  2:560600/1‑140 (MQ=255)

                                                                     tgatgaATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCcgc                                                          <  1:494787/140‑1 (MQ=255)

                                                                        tgaATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCa                                                       >  2:119222/1‑140 (MQ=255)

                                                                         gaATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCAt                                                      <  2:324884/140‑1 (MQ=255)

                                                                            tGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCg                                                   >  1:79182/1‑140 (MQ=255)

                                                                             gTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGt                                                  <  1:403638/140‑1 (MQ=255)

                                                                               tCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCg                                                >  1:34873/1‑140 (MQ=255)

                                                                                     ccAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATa                                          <  1:314255/140‑1 (MQ=255)

                                                                                            ggTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGtt                                   <  1:217749/140‑1 (MQ=255)

                                                                                                   aCATTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATtg                            <  2:96775/140‑1 (MQ=255)

                                                                                                     aTTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATtgat                          <  1:560600/140‑1 (MQ=255)

                                                                                                     aTTATCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATtgat                          <  1:460166/140‑1 (MQ=255)

                                                                                                        aTCTCCACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTgatgat                       >  1:253087/1‑140 (MQ=255)

                                                                                                             cACCGCCGACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTa                  >  2:546044/1‑140 (MQ=255)

                                                                                                                cgccgACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATcg               >  1:102659/1‑140 (MQ=255)

                                                                                                                 gccgACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATcgc              <  2:79182/140‑1 (MQ=255)

                                                                                                                  ccgACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATcgcg             <  2:206097/140‑1 (MQ=255)

                                                                                                                   cgACAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCgcgc            <  1:43034/140‑1 (MQ=255)

                                                                                                                      cAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCTGATCGCGCCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGTTgg         <  2:103867/140‑1 (MQ=255)

                                                                                                                      cAGCATTCGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTgg         <  1:317337/140‑1 (MQ=255)

                                                                                                                             cGGCAGATACTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCAt  <  2:102659/140‑1 (MQ=255)

TCGCAACTGTAGAGCAAGGTGCAGAAAACAGCTGGCTCGGCGCGTGGGCGAACAAAGAACAGGCCATTGTGATGAATGTTCAGCGCCAGCCCGGTGCTAACATTATCTCCACCGCCGACAGCATTCGGCAGATGCTGCCACAGCTCACTGAGAGTCTGCCGAAATCGGTGAAGGTGACAGTGCTTTCCGATCGCACCACCAATATCCGCGCATCCGTCGATGATACTCAGTTTGAATTGATGATGGCTATCGCGCTGGTAGTCAT  >  NC_000913/2156065‑2156329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A