breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
JC JC 751,781 IS5 (–) +4 bp coding (386‑389/2448 nt) ybgQ ← putative fimbrial usher protein YbgQ
RA 871,397 C→A A144E (GCA→GAA)  gsiC → glutathione ABC transporter membrane subunit GsiC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,106,111 C→A Q98K (CAG→AAG)  ymdB → 2'‑O‑acetyl‑ADP‑ribose deacetylase, regulator of RNase III activity
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,556,991 G→T A123S (GCA→TCA)  osmC → osmotically inducible peroxiredoxin
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,841,612 G→T P41P (CCG→CCT nudG → 5‑hydroxy‑CTP diphosphatase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,156,198 G→A M312I (ATG→ATA mdtB → multidrug efflux pump RND permease subunit MdtB
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,425,164 C→A A24S (GCG→TCG)  hisM ← lysine/arginine/ornithine ABC transporter/histidine ABC transporter, membrane subunit HisM
RA 2,449,195 G→T intergenic (+38/+33) smrB → / ← yfcO putative endonuclease SmrB/DUF2544 domain‑containing protein YfcO
RA 3,060,298 G→T T182T (ACG→ACT argP → DNA‑binding transcriptional dual regulator ArgP
RA 3,172,161 C→A M17I (ATG→ATT ygiZ ← conserved inner membrane protein YgiZ
RA 3,226,578 C→A F115L (TTC→TTA ygjI → putative transporter YgjI
RA 3,335,704 G→T A264E (GCG→GAG)  murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,503,593 G→T D143Y (GAC→TAC)  frlD → fructoselysine 6‑kinase
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,739,564 C→A intergenic (+37/‑141) malS → / → avtA alpha‑amylase/valine‑‑pyruvate aminotransferase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,877,579 G→T intergenic (‑114/+126) yidB ← / ← gyrB conserved protein YidB/DNA gyrase subunit B
RA 4,149,522 C→A Q248H (CAG→CAT eptC ← phosphoethanolamine transferase EptC
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,441,591 A→G Y196H (TAT→CAT)  msrA ← methionine sulfoxide reductase A
RA 4,466,730 G→T Q173K (CAG→AAG)  treR ← DNA‑binding transcriptional repressor TreR
RA 4,592,967 C→A S437R (AGC→AGA tsr → methyl‑accepting chemotaxis protein Tsr

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102979 3104051 1073 7 [6] [5] 10 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424230 3424525–3424242 13–756 9 [6] [6] 7 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374921 =NA (NA)3 (0.120) 3/192 2.9 NA intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = NA (NA)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375248 =NA (NA)17 (0.550) 7/240 2.2 NA noncoding (15/34 nt) other REP31g
?NC_000913 377507 = NA (NA)noncoding (16/34 nt) other REP32d
* ? NC_000913 1207790 =6 (0.170)40 (1.270) 33/246 0.1 82.2% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 12 (0.380)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078056 (0.170)22 (0.700) 17/246 0.7 71.8% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 12 (0.380)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 3363001 =58 (1.630)69 (1.950) 53/276 0.0 69.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.060)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)59 (1.660) 40/278 0.0 98.3% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 1 (0.030)coding (3907/4134 nt) rhsA rhs element protein RhsA