Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A6 F73 I1 R1	31	34.8	1203930	99.1%	1193094	139.7

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,503,593	G→T	D143Y (GAC→TAC)	frlD →	fructoselysine 6‑kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,503,593	0	G	T	100.0%	97.3 / NA	29	D143Y (GAC→TAC)	frlD	fructoselysine 6‑kinase
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (17/12); total (17/12)

AGTGAAGAGGATTACGCCTGGCTGGCGCAGTATGACATTGTGCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGT  >  NC_000913/3503479‑3503732

aGTGAAGAGGATTACGCCTGGCTGGCGCAGTATGACATTGTGCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCg                                                                                                                                    >  2:240669/1‑124 (MQ=255)

aGTGAAGAGGATTACGCCTGGCTGGCGCAGTATGACATTGTGCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCg                                                                                                                                    <  1:240669/124‑1 (MQ=255)

 gTGAAGAGGATTACGCCTGGCTGGCGCAGTATGACATTGTGCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCg                                                                                                                   <  1:61476/140‑1 (MQ=255)

              cGCCTGGCTGGCGCAGTATGACATTGTGCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGacac                                                                                                      <  1:451772/140‑1 (MQ=255)

                   ggctggcGCAGTATGACATTGTGCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTg                                                                                                 >  1:12341/1‑140 (MQ=255)

                   ggctggcGCAGTATGACATTGTGCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTg                                                                                                 >  1:124300/1‑140 (MQ=255)

                                        tgCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCAtc                                                                                          <  1:160723/126‑1 (MQ=255)

                                        tgCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCAtc                                                                                          >  2:160723/1‑126 (MQ=255)

                                        tgCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATtttgccttt                                                                            >  1:513380/1‑140 (MQ=255)

                                        tgCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATtttgccttt                                                                            >  2:202091/1‑140 (MQ=255)

                                               ggCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTccg                                                                     <  2:78236/140‑1 (MQ=255)

                                               ggCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTccg                                                                     >  2:150307/1‑140 (MQ=255)

                                                gCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTccgc                                                                    >  1:495881/1‑140 (MQ=255)

                                                              tGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACg                                                      >  1:496400/1‑140 (MQ=255)

                                                              tGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACg                                                      >  2:268967/1‑140 (MQ=255)

                                                               gCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAagaaga                                                        <  2:242416/137‑1 (MQ=255)

                                                               gCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAagaaga                                                        >  1:242416/1‑137 (MQ=255)

                                                                       cGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGc                                             >  1:48447/1‑140 (MQ=255)

                                                                         cATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGt                                           <  1:150307/140‑1 (MQ=255)

                                                                           ttCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACGGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCt                                         <  2:105940/140‑1 (MQ=255)

                                                                                       cACGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGc                             >  1:22550/1‑140 (MQ=255)

                                                                                         cGCTGCGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGa                           >  2:432997/1‑140 (MQ=255)

                                                                                              cGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGAttgtt                      >  1:113125/1‑140 (MQ=255)

                                                                                              cGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGAttgtt                      >  2:400515/1‑140 (MQ=255)

                                                                                              cGGGCAAACTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGAttgtt                      >  2:453103/1‑140 (MQ=255)

                                                                                                   aaaCTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGAGTCTGAAGATGAAAGCGATTGTTGCCCg                 <  1:268967/140‑1 (MQ=255)

                                                                                                      cTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTgg              <  2:22550/140‑1 (MQ=255)

                                                                                                      cTTACCGCTTTCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTgg              <  1:430396/140‑1 (MQ=255)

                                                                                                              tttCTACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGaa      <  1:400515/140‑1 (MQ=255)

                                                                                                                  gACTTCTCCGACAAGTGGTACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGt  <  2:255016/140‑1 (MQ=255)

AGTGAAGAGGATTACGCCTGGCTGGCGCAGTATGACATTGTGCACGCGGCAATCTGGGGACATGCGGAAGACGCATTCCCACAGCTGCACGCTGCGGGCAAACTTACCGCTTTCGACTTCTCCGACAAGTGGGACAGCCCGCTCTGGCAGACACTGGTGCCGCATCTCGATTTTGCCTTTGCCTCCGCACCGCAAGAAGACGAAACGCTGCGTCTGAAGATGAAAGCGATTGTTGCCCGTGGCGCAGGAACAGT  >  NC_000913/3503479‑3503732

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A